1887

Abstract

SUMMARY

RNA extracted from cells previously stimulated to synthesize the antiviral protein, interferon, causes species-specific interferon synthesis when added to heterospecific cell cultures. Our results confirm the report of De Maeyer-Guignard, De Maeyer & Montagnier (1972). We have used this observation to obtain a relative quantitative assay for the interferon messenger RNA activity. At appropriate concentrations, the yield of interferon is proportional to the concentration of RNA adsorbed to recipient cell cultures.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-27-2-225
1975-05-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/27/2/JV0270020225.html?itemId=/content/journal/jgv/10.1099/0022-1317-27-2-225&mimeType=html&fmt=ahah

References

  1. Aviv H., Packman S., Swan D., Ross J., Leder P. 1973; In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumor. Nature New Biology 241:174–176
    [Google Scholar]
  2. Berns A. J. M., Van kraaikamp M., Bloemendal H., Lane C. D. 1972; Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes. Proceedings of the National Academy of Sciences of the United States of America 69:1606–1609
    [Google Scholar]
  3. Bhoopalam N., Yakulis V., Costea N., Heller P. 1972; Surface immunoglobulins of circulating lymphocytes in mouse plasmacytoma. II. The influence of plasmacytoma RNA on surface immunoglobulins of lymphocytes. Blood 39:465–471
    [Google Scholar]
  4. Brachet J., Huez G., Hubert E. 1973; Microinjection of rabbit hemoglobin messenger RNA into amphibian oocytes and embryos. Proceedings of the National Academy of Sciences of the United States of America 70:543–547
    [Google Scholar]
  5. De maeyer-guignard J., De maeyer E., Montagnier L. 1972; Interferon messenger RNA: translation in heterologous cells. Proceedings of the National Academy of Sciences of the United States of America 69:1203–1207
    [Google Scholar]
  6. Diggelman H., Faust c. H., Mach B. 1973; Enzymatic synthesis of DNA complementary to purified 14S messenger RNA of immunoglobulin light chain. Proceedings of the National Academy of Sciences of the United States of America 70:693–696
    [Google Scholar]
  7. Duesberg P. H., Robinson W. S. 1965; Isolation of the nucleic acid of Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 54:794–800
    [Google Scholar]
  8. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis virus. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  9. Finter N. B. 1973; Interferon assay methods. In Interferons and Their Inducers pp. 139–142 Edited by Finter N. B. New York: American Elsevier;
    [Google Scholar]
  10. Gross K., Ruderman J., Jacobs-lorena M., Baglioni C., Gross P. R. 1973; Cell-free synthesis of histones directed by messenger RNA from sea urchin embryos. Nature New Biology 241:272–274
    [Google Scholar]
  11. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. 1971; The use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature, London 233:177–182
    [Google Scholar]
  12. Harris S. E., Means A. R., Mitchell W. M., O’malley B. W. 1973; Synthesis of [3H]DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Proceedings of the National Academy of Sciences of the United States of America 70:3776–3780
    [Google Scholar]
  13. Jacobs-lorena M., Baglioni c., Borun T. W. 1972; Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor. Proceedings of the National Academy of Sciences of the United States of America 69:2095–2099
    [Google Scholar]
  14. Kacian D. L., Spiegelman S., Bank A., Terada M., Metafora S., Dow L., Marks P. A. 1972; In Vitro synthesis of DNA components of human genes for globins. Nature New Biology 235:167–169
    [Google Scholar]
  15. Kacian D. L., Gambino R., Dow L., Grossbard E., Natta C., Ramirez F., Spiegelman S., Marks P. A., Bank A. 1973; Decreased globin messenger RNA in thalassemia detected by molecular hybridization. Proceedings of the National Academy of Sciences of the United States of America 70:1886–1890
    [Google Scholar]
  16. Kronenberg L. H. 1974; Cellular and molecular mechanisms controlling the interferon response. Ph.D. Thesis University of California, San Diego;
    [Google Scholar]
  17. Laskey R. A., Gurdon J. B., Crawford L. V. 1972; Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis. Proceedings of the National Academy of Sciences of the United States of America 69:3665–3669
    [Google Scholar]
  18. Mach B., Faust C., Vassali P. 1973; Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor. Proceedings of the National Academy of Sciences of the United States of America 70:451–455
    [Google Scholar]
  19. Moar V. A., Gurdon J. B., Lane C. D., Marbaix G. 1971; Translational capacity of living frog eggs and oocytes as judged by messenger RNA injection. Journal of Molecular Biology 61:93–103
    [Google Scholar]
  20. Orlova T. G., Georgadze J.J., Kognovitskaya A. I., Solovyov V. D. 1974; Interferon-inducing and interferon-translating functions of RNA isolated from Newcastle disease virus-infected cells. Acta Virologica 18:210–216
    [Google Scholar]
  21. Rhoads R. E., Mcknight G. s., Schimke R. T. 1973; Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Journal of Biological Chemistry 248:2031–2039
    [Google Scholar]
  22. Rollins J. W., Flickinger R. A. 1972; Collagen synthesis in Xenopus oocytes after injection of nuclear RNA of frog embryo. Science, New York 178:1204–1205
    [Google Scholar]
  23. Ross J., Aviv H., scolnick E., Leder P. 1972; In vitro synthesis of DNA complementary to purified rabbit globin mRNA. Proceedings of the National Academy of Sciences of the United States of America 69:264–268
    [Google Scholar]
  24. Schechter I. 1973; Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine. Proceedings of the National Academy of Sciences of the United States of America 70:2256–2260
    [Google Scholar]
  25. Schutz G., Beato ?., Feigelson P. 1973; Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones. Proceedings of the National Academy of Sciences of the United States of America 70:1218–1221
    [Google Scholar]
  26. Stevens R. H., Williamson A. R. 1972; Specific IgG mRNA molecules from myeloma cells in heterogeneous nuclear and cytoplasmic RNA containing poly-A. Nature, London 239:143–146
    [Google Scholar]
  27. Stewart W. E. 1974; Distinct molecular species of interferon. Virology 61:80–86
    [Google Scholar]
  28. Tan Y. H., Armstrong J. A., HO M. 1971; Intracellular interferon: kinetics of formation and release. Virology 45:837–840
    [Google Scholar]
  29. Taylor J. M., Schimke R. T. 1973; Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein- synthesizing system. Journal of Biological Chemistry 248:7661–7668
    [Google Scholar]
  30. Tomkins G. M., Levinson B. B., Baxter J. D., Dethlefsen L. 1972; Further evidence for post-transcriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells. Nature New Biology 239:9–14
    [Google Scholar]
  31. Tuohimaa P., Segal S. J., Koide S. S. 1972; Induction of avidin synthesis by RNA obtained from chick oviduct. Proceedings of the National Academy of Sciences of the United States of America 69:2814–2817
    [Google Scholar]
  32. Verma I. M., Temple G. F., Fan H., Baltimore D. 1972; In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nature New Biology 235:163–167
    [Google Scholar]
  33. Vilcek J., Ng M. H. 1971; Post-transcriptional control of interferon synthesis. Journal of Virology 7:588–594
    [Google Scholar]
  34. Watts R. L., Mathias A. P. 1967; The use of bentonite in the isolation of plant polyribosomes. Biochimica et Biophysica Acta 145:828–831
    [Google Scholar]
  35. Yamamoto Y., Tsukui K., Ohwaki M., Kawade Y. 1974; Electrophoretic characterization of purified mouse L cell interferon of high specific activity. Journal of General Virology 23:23–32
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-27-2-225
Loading
/content/journal/jgv/10.1099/0022-1317-27-2-225
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error