1887

Abstract

SUMMARY

virus () and virus () have been shown to be serogically unrelated. Amino acid compositions of the two virus capsids are compared and their capsid polypeptides have been examined by SDS-polyacrylamide gel electrophoresis. contained one major (Ø3) and two minor (Ø1 and Ø2) polypeptides with mol. wt. 87000, 125000 and 100000, while AfV-S contained one major (σ1) and one minor (σ2) polypeptide with mol. wt. 83000 and 78000 respectively. Evidence is presented that σ2 may be derived from σ1 polypeptide by proteolytic degradation . The mol. wt. of 4 and -S1a particles were found from sedimentation and diffusion coefficients to be 13.1 × 10 and 12.4 × 10 respectively. capsid was estimated to contain 120 molecules of polypeptide Ø3 and one molecule each of polypeptides Ø1 and Ø2, while capsid was estimated to contain 120 molecules of polypeptide Ø1.

It has been shown that S1a and S2a particles each contain a molecule of double-stranded with mol. wt. 2.24 × 10 (-224) and 2.76 × 10 (-276) respectively, whereas S1b and S2b particles each contain a molecule of -224 and -276 respectively, together with an additional molecule of double-stranded of mol. wt. 0.1 × 10. Evidence is presented that S4 particles contain two molecules of -224. S3 particles gave only -224 on extraction, but contain the equivalent of 1½ molecules of -224; the nature of these particles and other possible virus replicative intermediates is discussed. Double-stranded of mol. wt. 1.24 × 10 was derived from a newly described particle class, Fo.

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1975-05-01
2023-01-27
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References

  1. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., Himmelweit F., Ratti G., Sharp T. J., Planterose D. N. 1970; Antiviral activity of double-stranded RNA derived from a virus isolated from Aspergillus foetidus. Nature, London 227:505–507
    [Google Scholar]
  2. Berry E. A., Bevan E. A. 1972; A new species of double-stranded RNA from yeast. Nature, London 239:279–280
    [Google Scholar]
  3. Border D. J., Buck K. W., Chain E. B., Kempson-Jones G. F., Lhoas P., Ratti G. 1971; Viruses of Penicillium and Aspergillus species. Biochemical Journal 127:4–6 p
    [Google Scholar]
  4. Bozarth R. F., Wood H.A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex : two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  5. Buck K. W., Girvan R. F., Ratti G. 1973; Two serologically distinct double-stranded ribonucleic acid viruses isolated from Aspergillus niger. Biochemical Society Transactions 1:1138–1140
    [Google Scholar]
  6. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  7. Buck K. W., Kempson-Jones G. F. 1974; Capsid polypeptides of two viruses isolated from Penicillium stoloniferum. Journal of General Virology 22:441–445
    [Google Scholar]
  8. Goodwin T. W., Morton R. A. 1946; The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochemical Journal 40:628–632
    [Google Scholar]
  9. Hirs C. H. W. 1967 Determination of cystine as cysteic acid. In Methods in Enzymology vol. 11 pp. 59–62 Edited by Hirs C. H. W. London: Academic Press;
    [Google Scholar]
  10. Kallenbach N. R. 1968; Theory of thermal transitions in low molecular weight RNA chains. Journal of Molecular Biology 37:445–446
    [Google Scholar]
  11. Loviny T., Szekely M. 1973; Fingerprinting double-stranded non-radioactive RNA from a fungal virus. European Journal of Biochemistry 35:87-–94
    [Google Scholar]
  12. Markham R. 1962; The analytical ultracentrifuge as a tool for the investigation of plant viruses. Advances in Virus Research 9:241–270
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  14. Mayor H. D., Torikai K., Melnick J. L., Mandel M. 1969; Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions. Science, New York 166:1280–1282
    [Google Scholar]
  15. Mcmeekin T. L., Marshall K. 1952; Specific volumes of proteins and their relationship to their amino acid contents. Science, New York 116:142–143
    [Google Scholar]
  16. Ratti G., Buck K. W. 1972; Virus particles in Aspergillus foetidus: a multicomponent system. Journal of General Virology 14:165–175
    [Google Scholar]
  17. Symons R. H., Rees M. W., Short M. N., Markham R. 1963; Relationship between the ribonucleic acid and protein of some plant viruses. Journal of Molecular Biology 6:1–15
    [Google Scholar]
  18. Thomas A. J. 1970 In Automation, Mechanisation and Data Handling in Microbiology vol. 4 p. 107 Edited by Baillie A., Gilbert R. J. London: Academic Press;
    [Google Scholar]
  19. Weber K., Pringle J. R., Osborn N. 1972 Measurement of molecular weight by electrophoresis on SDS- acrylamide gel. In Methods in Enzymology vol. 16 pp. 3–27 Edited by Hirs C. H. W. London: Academic Press;
    [Google Scholar]
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