1887

Abstract

SUMMARY

The fate of early virus messenger in the cytoplasm of vaccinia-infected L cells has been studied during the first hour after infection. The is made in the virus core structure from which it is rapidly released. It accumulates in the polyribosome fraction, where at least 75% is bound to ribosomes through an -sensitive link. Three distinct structures have been identified as possible intermediates in virus polyribosome formation. The first is a ribonucleoprotein complex () in which virus is associated with cellular proteins. A complex having apparently similar properties, is formed when virus is added to a cytoplasmic extract . The other two structures may consist of an moiety associated with the small ribosomal subunit, or with a single ribosome. At least part of the isolated as appears to be a precursor of the virus messenger found in polyribosomes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-27-2-181
1975-05-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/27/2/JV0270020181.html?itemId=/content/journal/jgv/10.1099/0022-1317-27-2-181&mimeType=html&fmt=ahah

References

  1. Armstrong J. A., Metz D. H., Young M. R. 1973; The mode of entry of vaccinia virus into L cells. Journal of General Virology 21:533–537
    [Google Scholar]
  2. Baltimore D., Huang A. S. 1968; Isopycnic separation of subcellular components from poliovirus- infected and normal HeLa cells. Science, New York 162:572–574
    [Google Scholar]
  3. Baltimore D., Huang A. S. 1970; Interaction of HeLa cell proteins with RNA. Journal of Molecular Biology 47:263–273
    [Google Scholar]
  4. Beaud G., Kirn A., Gros F. 1972; In vitro protein synthesis directed by RNA transcribed from vaccinia DNA. Biochemical and Biophysical Research Communications 49:1459–1466
    [Google Scholar]
  5. Becker Y., Joklik W. K. 1964; Messenger RNA in cells infected with vaccinia virus. Proceedings of the National Academy of Sciences of the United States of America 51:577–585
    [Google Scholar]
  6. Belitsina N. V., Ovchinnikov L. P., Spirin A. S., Gendon, Yu. Z. Chernos V. I. 1968; Informosomes of HeLa cells infected with vaccinia virus. Molekulyarnaya Biologiya 2:727–735
    [Google Scholar]
  7. Dales S. 1963; The uptake and development of vaccinia virus in strain L cells followed with labelled viral deoxyribonucleic acid. Journal of Cell Biology 18:51–72
    [Google Scholar]
  8. Easterbrook K. B. 1966; Controlled degradation of vaccinia virions in vitro: an electron microscopic study. Journal of Ultrastructure Research 14:484–496
    [Google Scholar]
  9. Esteban M., Metz D. H. 1973; Early virus protein synthesis in vaccinia virus-infected cells. Journal of General Virology 19:201–216
    [Google Scholar]
  10. Fan H., Penman S. 1970; Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis. Journal of Molecular Biology 50:655–670
    [Google Scholar]
  11. Fournier F., Tovell D. R., Esteban M., Metz D. H., Ball L. A., Kerr I. M. 1973; The translation of vaccinia virus messenger RNA in animal cell-free systems. FEBS Letters 30:268–272
    [Google Scholar]
  12. Girard M., Baltimore D. 1966; The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA. Proceedings of the National Academy of Sciences of the United States of America 56:999–1002
    [Google Scholar]
  13. Haselkorn R., Rothman-Denes L. B. 1973; Protein synthesis. Annual Review of Biochemistry 42:397–438
    [Google Scholar]
  14. Holowczak J. A. 1972; Uncoating of poxviruses. I. Detection and characterization of subviral particles in the uncoating process. Virology 50:216–232
    [Google Scholar]
  15. Huang A. S., Baltimore D. 1970; Initiation of polyribosome formation in poliovirus-infected HeLa cells.Journal of Molecular Biology. 47:275–291
    [Google Scholar]
  16. Joklik W. K. 1962; The preparation and characteristics of highly purified radioactively labelled poxvirus. Biochemica et Biophysica Acta 61:290–301
    [Google Scholar]
  17. Joklik W. K., Becker Y. 1965; Studies on the genesis of polyribosomes. II. The association of nascent messenger RNA with the 40 S subribosomal particle. Journal of Molecular Biology 13:511–520
    [Google Scholar]
  18. Kates J. 1970; Transcription of the vaccinia virus genome and the occurrence of polyadenylic acid sequences in messenger RNA. Cold Spring Harbor Symposium on Quantitative Biology 35:743–752
    [Google Scholar]
  19. Kates J., Beeson J. 1970a; Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. Journal of Molecular Biology 50:1–18
    [Google Scholar]
  20. Kates J., Beeson J. 1970b; Ribonucleic acid synthesis in vaccinia virus. II. Synthesis of polyriboadenylic acid. Journal of Molecular Biology 50:19–33
    [Google Scholar]
  21. Kates J. R., Mcauslan B. R. 1967; Poxvirus DNA-dependent RNA polymerase activity. Proceedings of the National Academy of Sciences of the United States of America 58:134–141
    [Google Scholar]
  22. Lodish H. F. 1971; Alpha and beta globin messenger ribonucleic acid. Journal of Biological Chemistry 246:7131–7138
    [Google Scholar]
  23. Metz D. H., Esteban M. 1972; Interferon inhibits viral protein synthesis in L cells infected with vaccinia virus. Nature, London 238:385–388
    [Google Scholar]
  24. Metz D. H., Esteban M., Danielescu G. 1975; The effect of interferon on the formation of viral polyribosomes in L cells infected with vaccinia virus. Journal of General Virology 27:197–209
    [Google Scholar]
  25. Moss B. 1968; Inhibition of HeLa cell protein synthesis by the vaccinia virion. Journal of Virology 2:1028–1037
    [Google Scholar]
  26. Munyon W., Paoletti E., Grace J. T. Jun 1967; RNA polymerase activity in purified infectious vaccinia virus. Proceedings of the National Academy of Sciences of the United States of America 58:2280–2287
    [Google Scholar]
  27. Oda K. I., Joklik W. K. 1967; Hybridisation and sedimentation studies on ‘early’ and ‘late’ vaccinia messenger RNA. Journal of Molecular Biology 27:395–419
    [Google Scholar]
  28. Ovchinnikov L. P., Voronina A. S., Stepanov A. S., Velitsina N. V., Spirin A. S. 1968; InformOSOme-like complexes formed on addition of RNA to animal cell homogenates. Molekulyarnaya Biologiya 2:752–763
    [Google Scholar]
  29. Penman S., Vesco C., Penman M. 1968; Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells. Journal of Molecular Biology 34:49–69
    [Google Scholar]
  30. Perry R. P., Kelley D. E. 1966; Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labelled components. Journal of Molecular Biology 16:255–268
    [Google Scholar]
  31. Rosbash M., Penman S. 1971; Membrane-associated protein synthesis of mammalian cells. I. The two classes of membrane-associated ribosomes. Journal of Molecular Biology 59:227–241
    [Google Scholar]
  32. Schochetman G., Perry R. P. 1972; Characterization of the messenger RNA released from L cell polyribosomes as a result of temperature shock. Journal of Molecular Biology 63:577–590
    [Google Scholar]
  33. Sebring E. D., Salzman N. P. 1967; Metabolic properties of early and late vaccinia virus messenger ribonucleic acid. Journal of Virology 1:550–558
    [Google Scholar]
  34. Sheldon R., Kates J., Kelley D. E., Perry R. P. 1972; Polyadenylic acid sequences on 3’ termini of vaccinia messenger ribonucleic acid and mammalian nuclear and messenger ribonucleic acid. Biochemistry 11:3829–3834
    [Google Scholar]
  35. Siev M., Weinberg R., Penman S. 1969; The selective interruption of nucleolar RNA synthesis in HeLa cells by cordycepin. Journal of Cell Biology 41:510–520
    [Google Scholar]
  36. Spirin A. S. 1969; Informosomes. European Journal of Biochemistry 10:20–35
    [Google Scholar]
  37. Spohr G., Granboulan N., Morel C., Scherrer K. 1970; Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy. European Journal of Biochemistry 17:296–318
    [Google Scholar]
  38. Stanners C. P. 1966; The effect of cycloheximide on polyribosomes from hamster cells. Biochemical and Biophysical Research Communications 24:758–764
    [Google Scholar]
  39. Williamson R. 1973; The protein moieties of animal messenger ribonucleoproteins. FEBS Letters 37:1–6
    [Google Scholar]
  40. Woodson B. 1967; Vaccinia mRNA synthesis under conditions which prevent uncoating. Biochemical and Biophysical Research Communcations 27:169–175
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-27-2-181
Loading
/content/journal/jgv/10.1099/0022-1317-27-2-181
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error