1887

Abstract

SUMMARY

The fate of early virus messenger in the cytoplasm of vaccinia-infected L cells has been studied during the first hour after infection. The is made in the virus core structure from which it is rapidly released. It accumulates in the polyribosome fraction, where at least 75% is bound to ribosomes through an -sensitive link. Three distinct structures have been identified as possible intermediates in virus polyribosome formation. The first is a ribonucleoprotein complex () in which virus is associated with cellular proteins. A complex having apparently similar properties, is formed when virus is added to a cytoplasmic extract . The other two structures may consist of an moiety associated with the small ribosomal subunit, or with a single ribosome. At least part of the isolated as appears to be a precursor of the virus messenger found in polyribosomes.

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/content/journal/jgv/10.1099/0022-1317-27-2-181
1975-05-01
2022-01-25
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