1887

Abstract

SUMMARY

The effect of increasing concentrations of dimethylsulphoxide (DMSO) on the stability of native and reconstituted tobacco mosaic virus (TMV) has been investigated by means of electron microscopy and infectivity assay. The removal of protein subunits from TMV by DMSO was found to be a stepwise process. Subunits are first removed from the 3′ OH end of the rod but at about 72% DMSO the site at which uncoating occurs shifts to the 5′ end. Studies with reconstituted and partially reconstituted TMV confirm the stepwise nature of the uncoating process. Complete uncoating of reconstituted TMV was found to occur at smaller DMSO concentrations than that of native TMV.

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/content/journal/jgv/10.1099/0022-1317-26-3-295
1975-03-01
2022-01-24
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References

  1. Brakke M. K., Van Pelt N. 1969; Influence of bentonite, magnesium and polyamines on degradation and aggregation of tobacco mosaic virus. Virology 39:516–533
    [Google Scholar]
  2. Butler P. J. G., Klug A. 1971; Assembly of particles from RNA and disks of protein. Nature New Biology 229:47–50
    [Google Scholar]
  3. Diener T. O., Desjardins P. R. 1966; Factors affecting the stability of tobacco mosaic virus. I. Ribonuclease-induced protection of virus from alkaline degradation. Virology 29:15–25
    [Google Scholar]
  4. Fraenkel-Conrat H. 1957; Degradation of tobacco mosaic virus with acetic acid. Virology 4:1–4
    [Google Scholar]
  5. Guilley H., Stussi C., Thouvenel J. C., Pfeiffer P., Hirth L. 1972; Hydroxyapatite column chromoto-graphy of reconstitution products of TMV: some properties of the isolated fractions. Virology 49:475–485
    [Google Scholar]
  6. Kleinschmidt A., Zahn R. K. 1959; Uber Desoxyribonucleinsaüre-Molekulen in Protein Mischfilmen. Zeitschrift für Naturforschung 14b:770–779
    [Google Scholar]
  7. Lebeurier G., Hirth L. 1973; Tobacco mosaic virus reconstitution at low ionic strength. FEBS Letters 34:19–23
    [Google Scholar]
  8. Lonchampt M., Lebeurier G., Hirth L. 1972; Polymerization of tobacco mosaic virus protein in Na pyrophosphate. FEBS Letters 22:297–300
    [Google Scholar]
  9. May D. S., Knight C. A. 1965; Polar stripping of protein subunits from tobacco mosaic virus. Virology 25:502–507
    [Google Scholar]
  10. Nicolaieff A. 1969; Echantillonnage du DNA en solution par microscopie electronique. Journal de Microscopie %159–166
    [Google Scholar]
  11. Nicolaieff A., Lebeurier G., Hirth L. 1974; Electron microscopy and infectivity studies of the action of dimethylsulphoxide on two strains of tobacco mosaic virus. Journal of General Virology 22:43–53
    [Google Scholar]
  12. Ohno T., Nozu Y., Okada Y. 1971; Polar reconstitution of tobacco mosaic virus (TMV). Virology 44:510–516
    [Google Scholar]
  13. Stussi C., Lebeurier G., Hirth L. 1969; Partial reconstitution of tobacco mosaic virus. Virology 38:16–25
    [Google Scholar]
  14. Symington J. 1969a; Linear variation in stability of tobacco mosaic virus to stripping of protein by detergent. Virology 38:309–316
    [Google Scholar]
  15. Symington J. 1969b; Exposure of ribonucleic acid at both ends of tobacco mosaic virus by extensive protein stripping. Virology 38:317–323
    [Google Scholar]
  16. Symington I., Commoner B. 1967; Rod length in protein-stripped tobacco mosaic virus. Virology 32:1–10
    [Google Scholar]
  17. Thouvenel J. C., Guilley H., Stussi C., Hirth L. 1971; Evidence for polar reconstitution of TMV. FEBS Letters 16:204–206
    [Google Scholar]
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