1887

Abstract

Summary

When human rhinovirus type 2 attaches to HeLa cells, it first forms a reversible complex which can be dissociated by addition of EDTA to the medium. After prolonged incubation at 25 °C, most of the cell-associated virus progresses to an irreversible complex from which it cannot be eluted by EDTA, although some noninfectious material elutes spontaneously. The addition of a sufficient concentration of SDS to block cell-mediated eclipse of the virus does not block the formation of the irreversible complex at 25 °C, while virus adsorbed and incubated at 0 °C remains reversibly bound. The number of receptor sites per cell is not influenced by SDS, but at 0 °C cells appear to have fewer receptor sites than at physiological temperature.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-1-83
1974-10-01
2019-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-1-83
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error