1887

Abstract

SUMMARY

The response to various stimuli of herpes simplex virus-specified deoxypyrimidine kinase activity differs significantly from that of the thymidine kinase and deoxycytidine kinase of mammalian cells. However, the two phosphorylating activities of the HSV deoxypyrimidine kinase are themselves quite distinct in their biochemical behaviour.

It has been impossible to separate the HSV-coded thymidine and deoxycytidine phosphorylating activities by their sedimentation, electrophoretic or size characteristics. The evidence suggests that both deoxypyrimidine nucleosides are phosphorylated at the same active site.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-24-3-481
1974-09-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/24/3/JV0240030481.html?itemId=/content/journal/jgv/10.1099/0022-1317-24-3-481&mimeType=html&fmt=ahah

References

  1. Bresnick E., Mainige K. D., Bucano R., Burleson S. S. 1970; Studies on deoxythymidine kinase of regenerating rat liver and Escherichia coli. Cancer Research 30:2502–2506
    [Google Scholar]
  2. Bresnick E., Thompson U. B., Lyman K. 1966; Aggregation of deoxythymidine kinase in dilute solutions: properties of aggregated and disaggregated forms. Archives Biochemska Biophysika 144:352–359
    [Google Scholar]
  3. Cleaver J. E. 1971; Thymidine metabolism and cell kinetics. In North Holland Research Monograph, Frontiers of Biology vol 6 Edited by Neuberger A., Tatam E. L. Amsterdam: North Holland Publishing Company;
    [Google Scholar]
  4. Davis B. 1964; Gel electrophoresis. Disc electrophoresis. Method and application to serum proteins. Annals of New York Academy of Sciences 121:404–427
    [Google Scholar]
  5. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex deficient in thymidine kinase-inducing ability. Virology 22:493–502
    [Google Scholar]
  6. Durham J. D., Ives D. N. 1970; Deoxycytidine kinase. II. Purification and general properties of the calf-thymus enzyme. Journal of Biological Chemistry 245:2276–2284
    [Google Scholar]
  7. Hay J., Perera P. A. J., Morrison B. M., Gentry G. A., Subak-Sharpe B. H. 1970; Herpes virus-specified proteins. In Strategy of the Viral Genome355–376 (A Ciba Foundation Symposium) Edited by Wolstenhome G. E. W., O’Connor M. Edinburgh and London: Churchill Livingstone;
    [Google Scholar]
  8. Jamieson A. T., Gentry G. A., Subak-Sharpe J. H. 1974; Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. Journal of General Virology 24:465–480
    [Google Scholar]
  9. Kit S., Dubbs D. R. 1965; Properties of deoxythymidine kinase partially purified from noninfected and virus infected mouse fibroblast cells. Virology 26:16–27
    [Google Scholar]
  10. Kit S., Dubbs D. R., De Torres R. A., Melnick J. L. 1965; Enhanced thymidine kinase activity following infection of green monkey kidney cells by Simian adenoviruses, Simian papovavirus SV40 and an adenovirus-SV40 hybrid. Virology 27:453–455
    [Google Scholar]
  11. Kit S., Pieraiski L. J., Dubbs D. R. 1963; Effects of 5-fluorouracil, actinomycin D and mitomycin C on the induction of thymidine kinase by vaccinia-infected L-cells. Journal of Molecular Biology 7:497–510
    [Google Scholar]
  12. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus specific thymidine kinase on BHK 21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  13. Lowry O., Rosenbrough N., Farr A., Randall R. C. 1951; Protein measurement with a Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mcauslan B. R. 1963; The induction and repression of thymidine kinase in the poxvirus-infected HeLa cells. Virology 21:383–389
    [Google Scholar]
  15. Munyon W., Buchstraum R., Raoletti E., Kraiseburd E., Mann J. 1972; Electrophoresis of thymidine kinase activity synthesised by cells transformed by herpes simplex virus. Virology 49:683–689
    [Google Scholar]
  16. Munyon W., Kraiseburd E., Davis S., Mann B. 1971; Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet irradiated herpes simplex. Journal of Virology 7:813–820
    [Google Scholar]
  17. Perera P. A. J., Morrison S. M. 1970; Evidence for the induction of a new deoxycytidine kinase in cells infected with herpes virus. Biochemical Journal 117:21–22
    [Google Scholar]
  18. Roy-Burman P. 1970; Analogues of nucleic acid components. In Recent Results in Cancer Research vol 25 Berlin, Heidelberg and New York: Springer-Verlag;
    [Google Scholar]
  19. Sheinin R. 1966; Studies on the thymidine kinase activity of mouse embryo cells infected with polyoma virus. Virology 28:47–55
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-24-3-481
Loading
/content/journal/jgv/10.1099/0022-1317-24-3-481
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error