A double lysogenic strain w (β, ) induced with mitomycin C liberates phages β and . In addition the lysis of the organism is associated with the accumulation of a bacteriocin which was identified as phospholipase A. When the bacteria were cured of prophage they lost the enzyme production ability; the w (β) bacteria, named for convenience as bacteria, could be converted to phospholipase A production by phage infection. Phage and its mutant were very slowly adsorbed by bacteria in a liquid medium and did not propagate noticeably, but the presence of agar led to normal multiplication. The rate of lysogenic conversion was also enhanced by agar. The bulk of the enzyme produced by the convertant was isolated as a homomolecular protein and identified as phospholipase A.


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