1887

Abstract

Summary

Vesicular stomatitis virus (VSV) can form ‘pseudotype’ particles with neutralization antigen(s) derived from mouse or chicken leukaemia viruses. These pseudotype particles contain the genome of VSV, but show neutralization, host-range and interference specificity of the corresponding oncornavirus. Pseudotypes were also detected following the growth of VSV in two established cell lines derived from human tumours: in MaTu derived from mammary carcinoma and in Tu-135 derived from a sarcoma. No virus-like particles were detected in these cells by labelling with [H]-uridine followed by sucrose density gradient sedimentation. Media from these cultures did not contain DNA polymerase. Nevertheless, the capacity to produce VSV pseudotype was transmitted to some pseudotype-negative cells following their co-cultivation with X-irradiated, pseudotype-positive cells. Cell-free filtrates did not transmit the property. An antigen detected by immunofluorescence was also consistently transmitted. The VSV pseudotype produced in cells infected by co-cultivation possessed the same neutralization specificity as VSV pseudotype produced in the original tumour cells.

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/content/journal/jgv/10.1099/0022-1317-24-2-327
1974-08-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-24-2-327
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