1887

Abstract

SUMMARY

Vesicular stomatitis virus (VSV) can form ‘pseudotype’ particles with neutralization antigen(s) derived from mouse or chicken leukaemia viruses. These pseudotype particles contain the genome of VSV, but show neutralization, host-range and interference specificity of the corresponding oncornavirus. Pseudotypes were also detected following the growth of VSV in two established cell lines derived from human tumours: in MaTu derived from mammary carcinoma and in Tu-135 derived from a sarcoma. No virus-like particles were detected in these cells by labelling with [H]-uridine followed by sucrose density gradient sedimentation. Media from these cultures did not contain DNA polymerase. Nevertheless, the capacity to produce VSV pseudotype was transmitted to some pseudotype-negative cells following their co-cultivation with X-irradiated, pseudotype-positive cells. Cell-free filtrates did not transmit the property. An antigen detected by immunofluorescence was also consistently transmitted. The VSV pseudotype produced in cells infected by co-cultivation possessed the same neutralization specificity as VSV pseudotype produced in the original tumour cells.

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/content/journal/jgv/10.1099/0022-1317-24-2-327
1974-08-01
2021-10-27
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References

  1. Huang A. S., Besnier P., Chu L., Baltimore D. 1973; Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses. Journal of Virology 12:659–662
    [Google Scholar]
  2. Krontiris T. G., Soeiro R., Fields B. N. 1973; Host restriction of Friend leukemia virus. Role of the outer viral coat. Proceedings of the National Academy of Sciences of the United States of America 70:2549–2553
    [Google Scholar]
  3. Love D. N., Weiss R. 1974; Pseudotypes of vesicular stomatitis virus determined by exogenous and endogenous avian RNA tumor viruses. Virology 57:271–278
    [Google Scholar]
  4. McSharry J. J., Compans R. W., Choppin P. W. 1971; Proteins of vesicular stomatitis virus and of pheno-typically mixed vesicular stomatitis virus - simian virus 5 virions. Journal of Virology 8:722–729
    [Google Scholar]
  5. Mizutani S., Boettiger D., Temin H. M. 1970; A DNA-dependent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature, London 238:424–427
    [Google Scholar]
  6. Popovič M., Pontén I., Nilsson K., Bentsson A. 1973; Attempts to detect C-type particles in human tumour cell lines using conditional lethal mutant of VSV tl 17. Norwegian Journal of Zoology 21:188 (abstr)
    [Google Scholar]
  7. Temin H. M., Mizutani S. 1970; RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature, London 226:1211–1213
    [Google Scholar]
  8. Weiss R. A., Friis R. R., Katz E., Vogt P. K. 1971; Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology 46:920–938
    [Google Scholar]
  9. Widmaier R., Wildner G. P., Papsdorf G., Graffi I. 1974; Ueber eine neue, in vitro unbegrenzt wachsende Zellinie, MaTu, von Mamma-Tumorzellen des Menschen. Archiv fur Geschwulstforschung (in the press)
    [Google Scholar]
  10. Závada J. 1972a; Pseudotypes of vesicular stomatitis virus with the coat of murine leukaemia and of avian myeloblastosis virus. Journal of General Virology 15:183–191
    [Google Scholar]
  11. Závada J. 1972b; VSV pseudotype particles with the coat of avian myeloblastosis virus. Nature New Biology 240:122–124
    [Google Scholar]
  12. Závada J., Rosenbergová M. 1972; Phenotypic mixing of vesicular stomatitis virus with fowl plague virus. Acta virologica 16:103–114
    [Google Scholar]
  13. Závada J., Závadová Z., Malir A., Kočent A. 1972; VSV pseudotype produced in cell line derived from human mammary carcinoma. Nature New Biology 240:124–125
    [Google Scholar]
  14. Závada L., Závadová E. 1974; Complementation and phenotypic stabilisation of vesicular stomatitis virus temperature-sensitive and thermolabile mutants by avian myeloblastosis virus. Intervirology 2:25–32
    [Google Scholar]
  15. Zemek J., Svoboda J. 1973; Isolation and biological properties of two lines of human sarcoma cells. Meeting of the Czechoslovak Committee for Tissue Cultures, Kutna Horn 14 June
    [Google Scholar]
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