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Antibodies induced by the injection of alfalfa mosaic virus (AMV) or the protein of turnip crinkle virus (TCV) reacted with the viruses and their respective protein subunits in gel-diffusion tests. The ratios of virus-reactive antibodies to protein-reactive antibodies were different in two fractions obtained by chromatography of the sera on DEAE-Sephadex. The first DEAE-Sephadex fraction contained a greater proportion of virus-reactive antibodies than did the second fraction or the unfractionated antiserum. Electrophoretic mobility measurements at pH 8.6 showed that AMV and TCV had a greater negative charge per unit surface area than did their respective proteins.
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