1887

Abstract

SUMMARY

Dugbe virus multiplied in cell lines of pig and monkey origin producing little gross cytopathogenic effect, but prominent paranuclear, cytoplasmic inclusions were evident in stained preparations examined by light microscopy. Electron microscopic examination of thin sections of Dugbe virus-infected PS cells showed numerous virus particles 90 to 100 nm in diam. on the cell membrane and in cytoplasmic vacuoles. No virus particles were seen in inclusions, although these contained virus antigens demonstrable by immunofluorescence.

A marked prozone effect was seen in plaque titrations performed under carboxymethyl cellulose overlay. Dugbe virus-infected PS cells continued to divide and a chronically infected culture was established, which was resistant to superinfection with homologous virus. However, three togaviruses, Semliki Forest, yellow fever and Uganda S viruses, and Tahyna virus, multiplied almost as well in the Dugbe carrier culture as in control cells; Bwamba and Nairobi sheep disease viruses gave reduced yields in the Dugbe carrier cultures. It is suggested that the interference phenomenon reveals arbovirus relationships not demonstrable by conventional serological tests.

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1974-06-01
2022-12-08
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References

  1. Blaskovic D. 1969; Biology of arboviruses of the California complex and the Bunyamwera group. In Symposium Proceedings, Arboviruses of the California complex and the Bunyamwera group21–39 Edited by Bardos V. Bratislava: Publishing House of the Slovak Academy of Sciences;
    [Google Scholar]
  2. Bonsdorff C. H., Saikku P., Oker-Blom N. 1969; The inner structure of Uukuniemi and two Bunyamwera supergroup arboviruses. Virology 39:342–344
    [Google Scholar]
  3. Causey O. R. 1970; In Supplement to the Catalogue of Arthropod-borne viruses, No. 226. American Journal of Tropical Medicine and Hygiene 191123–1124
    [Google Scholar]
  4. Hirsch J. G., Fedorko M. E. 1968; Ultrastructure of human leucocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and ‘postfixation’ in uranyl acetate. Journal of Cell Biology 38:615–627
    [Google Scholar]
  5. Holmes L. H. 1971; Morphological similarity of Bunyamwera supergroup viruses. Virology 43:708–712
    [Google Scholar]
  6. Leibowitz A. 1963; The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. American Journal of Hygiene 78:173–180
    [Google Scholar]
  7. Madrid De. A. T., Porterfield J. S. 1969; A simple micro-culture method for the study of Group B arboviruses. Bulletin of the World Health Organization 40:113–121
    [Google Scholar]
  8. Murphy F. A., Harrison A. K., Whitfield S. G. 1973; ‘Bunyaviridae’ morphologic and morphogenetic similarities of Bunyamwera supergroup serological supergroup viruses and several other arthropodborne viruses. Intervirology 1:297–316
    [Google Scholar]
  9. Nasibov M. N., Smorodintsev A. A. 1968; An investigation into the nature of the intracellular inclusions formed by mumps virus, as determined by the combined use of immunofluorescence and methyl greenpyronin stain. Archiv. für die gesamte Virusforschung 24:319–331
    [Google Scholar]
  10. Nermut M. V. 1972; Further investigation on the fine structure of influenza virus. Journal of General Virology 17:317–331
    [Google Scholar]
  11. Paul S. D., Dandawate C. N. 1970; Polykaryocytosis in Vero cell cultures with Ganjam virus: an arbovirus. Indian Journal of Medical Research 58:556–560
    [Google Scholar]
  12. Porebska A., Pereira H. G., Armstrong J. A. 1968; Cytoplasmic inclusions and nuclear changes in cells infected with influenza B viruses. Journal of Medical Microbiology 1:145–151
    [Google Scholar]
  13. Reich E., Goldberg I. H. 1964; Actinomycin and nucleic acid function. In Progress in Nucleic Acid Research and Molecular Biology vol 3183–234 Edited by Davidson J. N., Cohn W. E. New York: Academic Press;
    [Google Scholar]
  14. Saikku P., Brummer-Korvenkontio M. 1973; Arboviruses in Finland. II. Isolation and characterisation of Uukuniemi virus, a virus associated with ticks and birds. American Journal of Tropical Medicine and Hygiene 22:390–399
    [Google Scholar]
  15. Taylor J. 1964; Inhibition of interferon action by actinomycin. Biochemical and Biophysical Research Communications 14:447–451
    [Google Scholar]
  16. Terpstra C. 1969 Nairobi sheep disease: studies on virus properties, epizootology and vaccination in Uganda Doctorate Thesis University of Utrecht;
    [Google Scholar]
  17. Theiler M., Downs W. G. 1973; In The Arthropod-borne Viruses of Vertebrates. New Haven and London: Yale University Press;
    [Google Scholar]
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