1887

Abstract

SUMMARY

The nuclei of Rous sarcoma cells were prepared from an established line of non-producer rat XC cells transformed with a Prague strain of Rous sarcoma virus (PR-RSV). Electron microscopic examination of the nuclear pellet showed a slight contamination with cytoplasmic debris and an absence of mitochondria. The DNA samples extracted from isolated nuclei and from whole XC cells were both assayed for infectivity in chicken cell cultures and found to contain about the same number of infective units per unit weight of DNA. Furthermore, the DNA from whole XC cells was set free by alkali and sedimented through an alkaline glycerol gradient in order to separate cellular DNA species according to sedimentation velocity. Under these conditions the infective RSV DNA consistently sedimented with the chromosomal 110S DNA and thus behaved as if covalently linked to the chromosomal DNA of XC cells. These results show that the infective virus DNA of non-producer RSV-transformed cells is carried in these cells as an integral part of the cellular chromosome.

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1974-06-01
2021-10-18
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References

  1. Bader A. V. 1973; Role of mitochondria in the production of RNA-containing tumor viruses. Journal of Virology 11:314–324
    [Google Scholar]
  2. Baltimore D. 1970; RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature, London 226:1209–1211
    [Google Scholar]
  3. Eigner J., Doty P. 1965; The native, denatured and renatured states of deoxyribonucleic acid. Journal of Molecular Biology 12:549–580
    [Google Scholar]
  4. Franze-FernÁndez M. T., Pogo A. O. 1971; Regulation of the nucleolar DNA-dependent RNA polymerase by amino acids in Ehrlich ascites tumor cells. Proceedings of the National Academy of Sciences of the United States of America 68:3040–3044
    [Google Scholar]
  5. Hill M. 1973; Input and output of genetic material in cells producing C-type RNA viruses. Biomedicine 18:453–458
    [Google Scholar]
  6. Hill M., Hillova J. 1971; Production virale dans les fibroblastes de Poule traités par l’acide désoxy-ribonucléique de cellules XC de Rat transformées par le virus de Rous. Comptes rendus hebdomadaires des seances de l’Académie des sciences 272:3094–3097
    [Google Scholar]
  7. Hill M., Hillova J. 1972a; Virus recovery in chicken cells treated with Rous sarcoma cell DNA. Nature New Biology 237:35–39
    [Google Scholar]
  8. Hill M., Hillova J. 1972b; Recovery of the temperature-sensitive mutant of Rous sarcoma virus from chicken cells exposed to DNA extracted from hamster cells transformed by the mutant. Virology 49:309–313
    [Google Scholar]
  9. Hillova J., Goubin G., Hill M. 1972; Transfection des fibroblastes de Poule par I’acide désoxyribonucléique dénaturé de cellules transformées de Rous. Comptes rendus hebdomadaires des séances de l’Académie des sciences 274:1970–1973
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Hložánek I., Svoboda J. 1972; Characterization of viruses obtained after cell fusion or transfection of chicken cells with DNA from virogenic mammalian Rous sarcoma cells. Journal of General Virology 17:55–59
    [Google Scholar]
  12. Koch J. 1973; Introduction of ‘nicks’ and ‘chops’ into human mitochondrial DNA in vivo and in vitro. European Journal of Biochemistry 33:98–103
    [Google Scholar]
  13. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  15. Meinke W., Hall M. R., Goldstein D. A., Kohne D. E., Lerner R. A. 1973; Physical properties of cytoplasmic membrane-associated DNA. Journal of Molecular Biology 78:43–56
    [Google Scholar]
  16. Montagnier L., Vigier P. 1972a; Un intermédiaire ADN infectieux et transformant du virus du sarcome de Rous dans les cellules de Poule transformées par ce virus. Comptes rendus hebdomadaires des séances de l’Académie des sciences 274:1977–1980
    [Google Scholar]
  17. Montagnier L., Vigier P. 1972b; Infectious DNA intermediate of an oncogenic RNA virus. Proceedings from the 6th Miles International Symposium for Molecular Biology212–217 Baltimore: John Hopkins University Press;
    [Google Scholar]
  18. Nonoyama M., Pagano J. S. 1972; Separation of Epstein-Barr virus DNA from large chromosomal DNA in non-virus-producing cells. Nature New Biology 238:169–171
    [Google Scholar]
  19. Pagano J. S., Hutchison C. A. III 1971; Small, Circular, viral DNA: preparation and analysis of SV40 and ϕX174 DNA. In Methods in Virology vol 5 pp 79–123 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  20. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. 1968; The integrated state of viral DNA in SV40 transformed cells. Proceedings of the National Academy of Sciences of the United States of America 60:1288–1295
    [Google Scholar]
  21. Studier F. W. 1965; Sedimentation studies of the size and shape of DNA. Journal of Molecular Biology 11:379–390
    [Google Scholar]
  22. Svoboda J., Hložánek I., Mach O. 1972; Detection of chicken sarcoma virus after transfection of chicken fibroblasts with DNA isolated from mammalian cells transformed with Rous virus. Folia Biologica, Prague 18:149–153
    [Google Scholar]
  23. Temin H. M. 1964; Nature of the provirus of Rous sarcoma. National Cancer Institute Monographs 17:557–570
    [Google Scholar]
  24. Temin H. M., Mizutani S. 1970; RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature, London 226:1211–1213
    [Google Scholar]
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