1887

Abstract

SUMMARY

Antisera raised in rabbits against RK13 cells infected with herpes simplex virus type 1 were capable of specifically precipitating proteins synthesized after infection of BHK-21 cells with the virus. Analysis of these immune precipitates by polyacrylamide gel electrophoresis demonstrated ≧ 15 polypeptides with mol. wt. from 25 to 100000. A number of these polypeptides were not detected in purified preparations of virus particles.

Precipitates formed with two ‘monoprecipitin’ antisera were also analysed. Antiserum to the structural antigen Band II precipitated a major polypeptide of mol. wt. 47000, which was glycosylated, and corresponded in mobility to a minor component polypeptide of the herpes virus particle. The other monoprecipitin antiserum, to the herpes-specified thymidine kinase, precipitated a polypeptide with a mol. wt. of 44000. The thymidine kinase polypeptide was not glycosylated.

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/content/journal/jgv/10.1099/0022-1317-22-2-171
1974-02-01
2022-01-25
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References

  1. Buchan A., Luff S., Wallis C. 1970a; Failure to demonstrate the interaction of subunits of thymidine kinase in cells simultaneously infected with herpesvirus and a kinaseless mutant. Journal of General Virology 9:239–242
    [Google Scholar]
  2. Buchan A., Watson D. H. 1969; The immunological specificity of thymidine kinase in cells infected with viruses of the herpes group. Journal of General Virology 4:461–463
    [Google Scholar]
  3. Buchan A., Watson D. H., Dubbs D. R., Kit S. 1970b; Serological study of a mutant herpesvirus unable to stimulate thymidine kinase. Journal of Virology 5:817–818
    [Google Scholar]
  4. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  5. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: Analysis by polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499–509
    [Google Scholar]
  6. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex virus deficient in thymidine kinase-inducing activity. Virology 22:493–502
    [Google Scholar]
  7. Geder L., Skinner G. R. B. 1971; Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescence technique. Journal of General Virology 12:179–182
    [Google Scholar]
  8. Holmes I. H., Watson D. H. 1963; An electron microscope study of the attachment and penetration of herpes virus in BHK-21 cells. Virology 21:112–123
    [Google Scholar]
  9. Honess R. W., Powell K. L., Robinson D. J., Sim C., Watson D. H. 1974; Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus. Journal of General Virology 22:159–169
    [Google Scholar]
  10. Keir H. M., Subak-Sharpe H., Shedden W., Watson D. H., Wildy P. 1966; Immunological evidence for a specific DNA polymerase produced after infection by herpes simplex virus. Virology 30:154–157
    [Google Scholar]
  11. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus specific thymidine kinase in BHK 21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  12. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. McConahey P. J., Dixon F. J. 1966; A method for the trace iodination of proteins for immunologic studies. International Archives of Allergy and Applied Immunology 29:185–189
    [Google Scholar]
  14. Morrison J. M., Keir H. M. 1967; Characterization of the deoxyribonuclease activity induced by infection with herpes simplex virus. Biochemical Journal 98:37 C
    [Google Scholar]
  15. Ornstein L. 1964; Disc electrophoresis. I. Background and theory. Annals of the New York Academy of Sciences 121:321–349
    [Google Scholar]
  16. Robinson D. J., Watson D. H. 1971; Structural proteins of herpes simplex virus. Journal of General Virology 10:163–171
    [Google Scholar]
  17. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  18. Sim C., Watson D. H. 1973; The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. Journal of General Virology 19:217–233
    [Google Scholar]
  19. Tokumaru T. 1965; Studies of herpes simplex virus by the gel diffusion technique. II. The characterization of viral and soluble precipitating antigens. Journal of Immunology 95:189–195
    [Google Scholar]
  20. Watson D. H. 1962; Particle counts on herpes virus in phosphotungstate negatively stained preparations. In Electron Microscopy. 5th International Congress for Electron Microscopy 2: paper X4 Edited by Breese S. S.
    [Google Scholar]
  21. Watson D. H. 1969; The separation of herpes virus specific antigens by polyacrylamide gel electrophoresis. Journal of General Virology 4:151–161
    [Google Scholar]
  22. Watson D. H., Shedden W. I. H., Elliott A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology II:399–408
    [Google Scholar]
  23. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
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