1887

Abstract

SUMMARY

Infectious pancreatic necrosis (IPN) virus has been purified and some physicochemical properties of its nucleic acid and protein moieties have been determined. The buoyant density of the virus particle in CsCl is 1.35 g/ml; degraded particle banded at a density of 1.31 g/ml. The virus nucleic acid was found to be double-stranded, according to several different criteria, namely resistance to pancreatic RNase, low density in CsSO (1.615), sharp melting curve with a of 89 °C in hypotonic buffer, and base composition. In a sucrose gradient the bulk of the virus RNA banded as a single peak (14 S); a small part of the virus nucleic acid was found at the top of the gradient. By gel electrophoresis virus RNA was resolved into three peaks corresponding to double-stranded molecules with mol. wt. ranging from 2.85 × 10 to 2.55 × 10. Gel analysis of virus proteins revealed three polypeptides of mol. wt. 80, 50 and 30 × 10, representing respectively 3%, 68% and 29% of the total protein. On the basis of these results IPN virus appears to be related to some reo-like viruses, having a single protein shell and a single class of double-stranded RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-3-485
1973-12-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/3/JV0210030485.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-3-485&mimeType=html&fmt=ahah

References

  1. Amano Y., Katagiri S., Ishida N., Watanabe Y. 1971; Spontaneous degradation of Reovirus capsid into subunits. Journal of Virology 8:805–808
    [Google Scholar]
  2. Argot J. E. 1969 Ph.D. Thesis Lehigh University; Bethlehem, Pa. Microfilms at Ann Arbor, Mich:
  3. Bellamy A. R. L., August J. T., Joklik W. K. 1967; Studies on Reovirus RNA. I. Characterisation of Reovirus genome RNA. Journal of Molecular Biology 29:1–17
    [Google Scholar]
  4. Bishop J. M., Summers D., Levintow L. 1965; Characterisation of ribonuclease resistant RNA from poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:1273–1281
    [Google Scholar]
  5. Cohen J., Scherrer R. 1972; Structure de la capside du virus de la Necrose Pancreatique Infectieuse (NPI). Compte rendu de I’Academie des Sciences, Paris 274:1222–1225
    [Google Scholar]
  6. Diener T. O., Schneider I. R. 1968; Virus degradation and nucleic acid release in single phase phenol systems. Archives of Biochemistry and Biophysics 124:401–412
    [Google Scholar]
  7. Els H. J., Verwoerd D. W. 1969; Morphology of the bluetongue virus. Virology 38:213–219
    [Google Scholar]
  8. Gravell M., Malsberger R. G. 1965; A permament cell line from fathead minnow (Pimephales promelas). Annals of the New York Academy of Sciences 126:555–565
    [Google Scholar]
  9. Harel M., Montagnier L. 1971; Homology of double stranded RNA from rat liver cells with the cellular genome. Nature, London 229:106–108
    [Google Scholar]
  10. Kalmakoff J., Lewandowski L. J., Black D. R. 1969; Comparison of ribonucleic acid subunits of Reovirus, cytoplasmic polyhedrosis virus, and wound tumor virus. Journal of Virology 4:851–856
    [Google Scholar]
  11. Kelly P. K., Loh P. C. 1972; Electron microscopical and biochemical characterisation of infectious pancreatic necrosis virus. Journal of Virology io:824–834
    [Google Scholar]
  12. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  13. Loh P. C., Shatkin A. J. 1968; Structural proteins of reoviruses. Journal of Virology 2:1353–1359
    [Google Scholar]
  14. Malsberger R. G., Cerini C. P. 1965; Multiplication of infectious pancreatic necrosis virus. Annals of the New York Academy of Sciences 126:320–327
    [Google Scholar]
  15. Montagnier L. 1968; Presence d’un acide ribonucleique double chaine dans des cellules animales. Compte rendu de I’Academie des Sciences, Paris D 267:1417
    [Google Scholar]
  16. Moss L. H., Gravell M. 1969; Ultrastructure and sequential development of infectious pancreatic necrosis virus. Journal of Virology 3:52–58
    [Google Scholar]
  17. Nicholson B. L. 1971; Macromolecular synthesis in RTG2 cells following infection with Infectious Pancreatic Necrosis (IPN) virus. Journal of General Virology 13:369–372
    [Google Scholar]
  18. Scherrer R., Cohen J. 1971 Proceedings of the znd International Congress of Virology p. 263 Edited by Melnick J. L. Basel: Karger;
    [Google Scholar]
  19. Scherrer K., Marcaud L., Zajdela F., Breckenridge B., Gros F. 1966; Etude des ARN nucleaires et cytoplasmiques a marquage rapide dans les cellules erythropoietiques aviaires differenciees. Bull. Soc. Chim. Biol 48:1037
    [Google Scholar]
  20. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  21. Shatkin A. J. 1965; Inactivity of purified reovirus RNA as a template for E. coli polymerase in vitro. Proceedings of the National Academy of Sciences of the United States of America 54:1721–1728
    [Google Scholar]
  22. Shatkin A. J., Sipe J. D., Loh P. C. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2:986–991
    [Google Scholar]
  23. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top component and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  24. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in polio virus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  25. Vasquez C., Tournier P. 1962; The morphology of reovirus. Virology 17:503–510
    [Google Scholar]
  26. Verwoerd D. W. 1969; Purification and characterisation of Bluetongue virus. Virology 38:203–212
    [Google Scholar]
  27. Wolf K., Quimby M. C. 1962; Established eurythermic line of fish cells in vitro. Science, New York 135:1065–1066
    [Google Scholar]
  28. Wolf K., Quimby M. C. 1967; Infectious pancreatic necrosis (IPN), its diagnosis, identification, detection and control. Rivista italiana di piscicoltura e ittopatologia, Padua A no (4):76–84
    [Google Scholar]
  29. Wood H. A., Bozarth R. F. 1972; Properties of virus like particles of Penicillium chrysogenum: One double-stranded RNA molecule per particle. Virology 47:604–609
    [Google Scholar]
  30. Wood H. A., Streissle G. 1970; Wound tumor virus: purification and fractionation of the doublestranded ribonucleic acid. Virology 44:329–334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-3-485
Loading
/content/journal/jgv/10.1099/0022-1317-21-3-485
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error