1887

Abstract

SUMMARY

The IBT-dependent mutant of vaccinia virus needs IBT for its growth. IBT is not essential for the synthesis of virus DNA, but the formed DNA does not become resistant to deoxyribonuclease in its absence. Both ‘early’ and ‘late’ virus proteins are synthesized in cells infected with the mutant, but in the absence of IBT one of the virus core structural polypeptides, which is normally formed from a higher mol. wt. precursor, is not made. These findings suggest that the IBT-dependent step of the mutant occurs earlier in the maturation process of the virus than does the block produced by IBT with the wild-type strain.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-3-477
1973-12-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/3/JV0210030477.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-3-477&mimeType=html&fmt=ahah

References

  1. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science, New York 130:432–437
    [Google Scholar]
  2. Fairbanks G. Jun, Levinthal G., Reeder R. H. 1965; Analysis of 14C-labelled proteins by disc electrophoresis. Biochemical and Biophysical Research communications 20:393–399
    [Google Scholar]
  3. Holowczak J. A., Joklik W. K. 1967; Studies on the structural proteins of vaccinia virus. I. Structural proteins of virions and cores. Virology 33:717–725
    [Google Scholar]
  4. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. Journal of Molecular Biology 10:452–474
    [Google Scholar]
  5. Katz E., Margalith E., Winer B., Goldblum N. 1973a; Synthesis of vaccinia viral polypeptides in the presence of isatin P thiosemicarbazone. Antimicrobial Agents and Chemotherapy 4:44–48
    [Google Scholar]
  6. Katz E., Moss B. 1970a; Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proceedings of the National Academy of Sciences of the United States of America 66:677–684
    [Google Scholar]
  7. Katz E., Moss B. 1970b; Vaccinia virus structural polypeptide derived from a high molecular weight precursor: formation and integration into virus particles. Journal of Virology 6:717–726
    [Google Scholar]
  8. Katz E., Winer B., Margalith E., Goldblum N. 1973b; Isolation and characterization of an IBT-dependent mutant of vaccinia virus. Journal of General Virology 19:161–164
    [Google Scholar]
  9. Moss B. 1968; Inhibition of HeLa cell protein synthesis by the vaccinia virion. Journal of Virology 2:1028–1037
    [Google Scholar]
  10. Moss B., Rosenblum E. N., Katz E., Grimley P. M. 1969; Rifampicin - a specific inhibitor of vaccinia virus assembly. Nature, London 224:1280–1284
    [Google Scholar]
  11. Moss B., Salzman N. P. 1968; Sequential protein synthesis following vaccinia virus infection. Journal of Virology 2:1016–1027
    [Google Scholar]
  12. Salzman N. P. 1960; The rate of formation of vaccinia deoxyribonucleic acid and vaccinia virus. Virology 10:150–152
    [Google Scholar]
  13. Salzman N. P., Sebring E. D. 1967; Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. Journal of Virology 1:16–23
    [Google Scholar]
  14. Shatkin A. J. 1963; Actinomycin D and vaccinia virus infection of HeLa cells. Nature, London 199:357–358
    [Google Scholar]
  15. Sheffield F. W., Bauer D. J., Stephenson S. 1960; The protection of tissue cultures by isatin β thiosemi-carbazone from the cytopathic effects of certain pox viruses. British Journal of Experimental Pathology 41:638–647
    [Google Scholar]
  16. Summers D. F., Maizel J. V., Darnell J. E. Jr 1965; Evidence for virus specific non-capsid proteins in poliovirus infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  17. Woodson B., Joklik W. K. 1965; The inhibition of vaccinia virus multiplication by isatin β thiosemi-carbazone. Proceedings of the National Academy of Sciences of the United States of America 54:946–953
    [Google Scholar]
  18. Zylber E. A., Penman S. 1970; The effect of high ionic strength on monomers, polyribosomes and puromycin-treated polyribosomes. Biochimica et biophysica acta 204:221–229
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-3-477
Loading
/content/journal/jgv/10.1099/0022-1317-21-3-477
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error