1887

Abstract

SUMMARY

This paper describes the physico-chemical properties of Nodamura virus. The virus infects mice and high yields (10 LD/mg) were obtained from the muscle tissue. To allow for greater ease and accuracy in analysing the RNA, the virus was labelled by inoculating mice with [P]-phosphate during the incubation period. Purified virus was obtained from the muscle extracts by ammonium sulphate precipitation, differential ultracentrifuging and sedimentation of the SDS-disrupted virus pellet in a sucrose gradient. The virus particles were 29 nm in diam., sedimented at approximately 135S in sucrose gradients and contained two species of RNA which sedimented at 22S and 15S. These values correspond to mol. wt. of 1.0 and 0.5 × 10. Similar mol. wt. were obtained by polyacrylamide gel electrophoresis. The base ratios of the two RNA species were: 22S: A = 22.4, C = 27.0, G = 27.6, U = 23.0; 15S: A = 24.8, C = 28.2, G = 22.8, U = 24.2. Each RNA had low infectivity in mice but this was enhanced about 100-fold by mixing the two species. The RNAs may be present in two distinct particles since extraction of the 135S peak with phenol gave the 22S RNA alone, whereas phenol-SDS extraction liberated both the 22S and 15S species. Centrifuging in caesium chloride also fractionated the virus into components which contained either the 22S or 15S RNA. Preliminary evidence suggests that there is one major polypeptide, mol. wt. 35 × 10, and two minor polypeptides in the virus.

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1973-11-01
2024-12-07
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References

  1. Bailey L., Scott H. A. 1973; The pathogenicity of Nodamura virus for insects. Nature, London 241:545
    [Google Scholar]
  2. Boedtker H. 1968; Dependence of the sedimentation coefficient on molecular weight after reaction with formaldehyde. Journal of Molecular Biology 35:61–70
    [Google Scholar]
  3. Brown F., Hull R. 1973; Comparative virology of the small RNA viruses. Journal of General Virology 20: (supplement) 43–60
    [Google Scholar]
  4. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:78–84
    [Google Scholar]
  5. Davidson J. N., Smellie R. M. S. 1952; Phosphorus compounds in the cell. 3. The incorporation of radioactive phosphorus into the ribonucleotide fraction of liver tissue. Biochemical Journal 52:599–606
    [Google Scholar]
  6. Murphy F. A., Scherer W. F., Harrison A. K., Dunne H. W., Gary G. W. 1970; Characterization of Nodamura virus, an arthropod transmissible picornavirus. Virology 40:1008–1021
    [Google Scholar]
  7. Newman J. F. E., Rowlands D. J., Brown F. 1973; A physico-chemical sub grouping of the mammalian picornaviruses. Journal of General Virology 18:171–180
    [Google Scholar]
  8. Scherer W. F., Hurlbut H. S. 1967; Nodamura virus from Japan: a new and unusual arbovirus resistant to diethyl ether and chloroform. American Journal of Epidemiology 86:271–285
    [Google Scholar]
  9. Scherer W. F., Verna J. E., Richter G. W. 1968; Nodamura virus, an ether and chloroform-resistant arbovirus from Japan. American Journal of Tropical Medicine and Hygiene 17:120–128
    [Google Scholar]
  10. Spirin A. S. 1964 In Macromolecular Structure of Ribonucleic Acids pp 13 Edited by Steikol J. A. New York: Reinhold Publishing Corporation;
    [Google Scholar]
  11. Strohmaier K. 1971 In Foot-and-Mouth Disease Virus. Session 15 Proceedings of the 2nd International Congress for Virology Edited by Melnick J. L. Basel: Karger;
    [Google Scholar]
  12. Takebe L., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 64:843–848
    [Google Scholar]
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