1887

Abstract

Summary

It has been shown by competition hybridization experiments that common sequences of about 600 nucleotides are present in TRV long and short particle RNA species. Heteroduplex molecules have been formed by the hybridization of short particle RNA strands with long complementary strands derived from tissue infected with a ‘defective isolate’ of the virus. The heteroduplex molecules were shown to have a lower melting temperature than either the long-long or short-short homoduplexes, indicating some mismatching of base pairs. The results are discussed in the light of our present knowledge about the replication of viruses.

Theoretical equations have been derived to describe RNA-RNA competition hybridization and duplex formation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-2-285
1973-11-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-2-285
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error