1887

Abstract

SUMMARY

It has been shown by competition hybridization experiments that common sequences of about 600 nucleotides are present in TRV long and short particle RNA species. Heteroduplex molecules have been formed by the hybridization of short particle RNA strands with long complementary strands derived from tissue infected with a ‘defective isolate’ of the virus. The heteroduplex molecules were shown to have a lower melting temperature than either the long-long or short-short homoduplexes, indicating some mismatching of base pairs. The results are discussed in the light of our present knowledge about the replication of viruses.

Theoretical equations have been derived to describe RNA-RNA competition hybridization and duplex formation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-2-285
1973-11-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/2/JV0210020285.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-2-285&mimeType=html&fmt=ahah

References

  1. Butler P. J. G., Klug A. 1971; Assembly of the particle of tobacco mosaic virus from RNA and discs of protein. Nature New Biology 229:47–50
    [Google Scholar]
  2. Content J., Duesberg P. M. 1971; Base sequence differences among the ribonucleic acids of influenza virus. Journal of Molecular Biology 62:273–285
    [Google Scholar]
  3. Cooper J. L., Mayo N. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  4. Cory S., Adams J. M., Spahr P. F., Rensing U. 1972; Sequence of 51 nucleotides at the 3′-end of R17 bacteriophage RNA. Journal of Molecular Biology 63:41–56
    [Google Scholar]
  5. Darby G., Minson A. C. 1972; The structure of tobacco rattle virus ribonucleic acids: nature of the 3′-terminal nucleosides. Journal of General Virology 14:199–206
    [Google Scholar]
  6. Fritsch C., Witz J., Abou Haidar M., Hirth L. 1972; Polymerisation of tobacco rattle virus protein. FEBS Letters 29:211–214
    [Google Scholar]
  7. Frost R. R., Harrison B. D., Woods R. D. 1967; Apparent symbiotic interaction between particles of tobacco rattle virus. Journal of General Virology 1:57–69
    [Google Scholar]
  8. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620
    [Google Scholar]
  9. Hindley J., Staples D. M. 1969; Sequence of a ribosome binding site in bacteriophage Qβ RNA. Nature, London 224:964–967
    [Google Scholar]
  10. Horst J., Content J., Mandeles S., Fraenkel-Conrat H., Duesberg P. 1972; Distinct oligonucleotide patterns of distinct influenza virus RNAs. Journal of Molecular Biology 69:209–215
    [Google Scholar]
  11. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. Journal of Molecular Biology 18:204–214
    [Google Scholar]
  12. Lister R. M. 1966; Possible relationships of virus specific products of tobacco rattle virus infections. Virology 28:350–353
    [Google Scholar]
  13. Lister R. M. 1968; Functional relationships between virus specific products of infection by viruses of the tobacco rattle type. Journal of General Virology 2:43–58
    [Google Scholar]
  14. Minson A. C., Darby G. 1973; A study of sequence homology between tobacco rattle virus ribonucleic acids. Journal of General Virology 19:253–262
    [Google Scholar]
  15. Prives C. L., Silverman P. M. 1972; Replication of RNA viruses: structure of a 6S RNA synthesised by the Qβ polymerase. Journal of Molecular Biology 71:657–670
    [Google Scholar]
  16. Sänger H. L. 1968; Characteristics of tobacco rattle viruses: evidence that its two particles are functionally defective and mutually complementing. Molecular and General Genetics 101:346–367
    [Google Scholar]
  17. Sänger H. L. 1969; Functions of two particles of tobacco rattle virus. Journal of Virology 3:304–312
    [Google Scholar]
  18. Semančik J. S., Reynolds D. A. 1969; Assembly of protein and nucleoprotein particles from extracted tobacco rattle virus protein and RNA. Science, New York 164:559–560
    [Google Scholar]
  19. Spiegelman S. 1971; Extracellular strategies of a replicating RNA genome. In Strategy of the Viral Genome PP 45–73 Edited by Wolstenholme G. E. W., O’Connor M. London: Churchill & Livingstone;
    [Google Scholar]
  20. Simmons D. T., Strauss J. H. 1972; Replication of Sindbis virus. II. Multiple forms of double-stranded RNA isolated from infected cells. Journal of Molecular Biology 71:615–631
    [Google Scholar]
  21. Staples D. H., Hindley J. 1971; Ribosome binding site of Qβ polymerase cistron. Nature New Biology 234:211–212
    [Google Scholar]
  22. Steitz J. A. 1969; Polypeptide chain initiation: nucleotide sequences of the three ribosome binding sites in bacteriophage R17 RNA. Nature, London 224:957–964
    [Google Scholar]
  23. Tollin P., Wilson H. R. 1971; Some observations on the campinas strain of tobacco rattle virus. Journal of General Virology 13:433–440
    [Google Scholar]
  24. Van Kammen A. 1971; Cowpea mosaic virus, un virus au genome divise. Physiologie Végétate 9:479–485
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-2-285
Loading
/content/journal/jgv/10.1099/0022-1317-21-2-285
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error