Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 21, Issue 2

Cytopathological Changes and Extractable Infectivity in Leaves Infected with Carrot Mottle Virus No Access

Abstract

SUMMARY

Cytopathological changes induced by carrot mottle virus in systemically infected cells of plants grown at 17 °C and illuminated at 4000 lux for 8 h/day, were studied in relation to the infectivity of buffer and phenoltreated extracts. Changes were most obvious in palisade cells. About six days after inoculation, tubules appeared in the cytoplasm, associated with the plasmodesmata. Later the tubules, some of which became sheathed by cell wall material forming plasmodesmatal outgrowths, extended towards the vacuole, others towards the nucleus, causing invaginations in it. Endoplasmic reticulum and Golgi bodies increased, especially in areas adjacent to the nuclei, and complexes of cytoplasmic tubules with endoplasmic reticulum were observed. Membrane-bound particles, some with densely-staining central spots, appeared in the vacuole close to the tonoplast and reached their maximum number after 8 to 9 days. Sometimes they were clustered where a plasmodesmatal tubule or outgrowth came close to the tonoplast.

Tests with the phenol-treated and buffer extracts showed that the cells contained both labile and stable forms of infectivity. The former predominated early in systemic infection (about 5 to 7 days after inoculation) and then declined, while the amount of stable infectivity increased, reaching a maximum after 8 to 9 days. It is suggested that the labile form of infectivity consists of RNase-sensitive material, probably nucleocapsids, which become stable (RNase-resistant) when they receive a protective envelope on entry into the vacuole.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1973
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-2-269
1973-11-01
2025-01-15
Loading full text...

Full text loading...

References

  1. Burgess J. 1969; Two cytoplasmic features of prophase in wheat root cells. Planta 87:259–270
     [Google Scholar]
  2. Conti G. G., Vegetti G., Bassi M., Favali M. A. 1972; Some ultrastructural and cytochemical observations on Chinese cabbage leaves infected with cauliflower mosaic virus. Virology 47:644–700
     [Google Scholar]
  3. Frisch-Niggemeyer W., Reddi K. K. 1957; Studies on ribonuclease in tobacco leaves. Ⅰ. Purification and properties. Biochimica et biophysica acta 26:40–46
     [Google Scholar]
  4. Gerola F. M., Bassi M. 1966; An electron microscopy study of leaf vein tumours from maize plants experimentally infected with maize rough dwarf virus. Caryologia 19:13–40
     [Google Scholar]
  5. Grimley P. M., Levin J. G., Berezesky I. K., Friedman R. M. 1972; Specific membranous structures associated with the replication of group A arboviruses. Journal of Virology 10:492–503
     [Google Scholar]
  6. Harrison B. D., Stefanac Z., Roberts I. M. 1970; Role of mitochondria in the formation of X-bodies in cells of Nicoliana clevelandii infected by tobacco rattle viruses. Journal of General Virology 6:127–140
     [Google Scholar]
  7. Hills G. J., Plaskitt A. 1968; A protein stain for the electron microscopy of small isometric plant virus particles. Journal of Ultrastructure Research 25:323–329
     [Google Scholar]
  8. Hooper G. R., Wiese M. V. 1972; Cytoplasmic inclusions in wheat affected by wheat spindle streak mosaic. Virology 47:664–672
     [Google Scholar]
  9. Huber J. D., Parker F., Odland G. F. 1968; A basic fuchsin and alkalinized methylene blue rapid stain for epoxy-embedded tissue. Stain Technology 43:83–87
     [Google Scholar]
  10. Jones A. T., Kinninmonth A. M., Roberts I. M. 1973; Ultrastructural changes in differentiated leaf cells infected with cherry leaf roll virus. Journal of General Virology 18:61–64
     [Google Scholar]
  11. Kim K. S., Fulton J. P. 1971; Tubules with virus-like particles in leaf cells infected with bean pod mottle virus. Virology 43:329–337
     [Google Scholar]
  12. Kim K. S., Fulton J. P. 1972; Fine structure of plant cells infected with bean pod mottle virus. Virology 49:112–121
     [Google Scholar]
  13. Kitajima E. W., Lauritis J. A. 1969; Plant virions in plasmodesmata. Virology 37:681–684
     [Google Scholar]
  14. Loening U. E., Ingle J. 1967; Diversity of RNA components in green plant tissues. Nature, London 215:363–367
     [Google Scholar]
  15. Murant A. F., Goold R. A., Roberts I. M., Cathro J. 1969; Carrot mottle – a persistent aphid-borne virus with unusual properties and particles. Journal of General Virology 4:329–341
     [Google Scholar]
  16. Reddi K. K., Mauser L. 1965; Studies on the formation of tobacco mosaic virus ribonucleic acid. Ⅵ. Mode of degradation of host ribonucleic acid to ribonucleosides and their conversion to ribonucleoside 5′-phosphates. Proceedings of the National Academy of Sciences of the United States of America 53:607–613
     [Google Scholar]
  17. Roberts I. M., Harrison B. D. 1970; Inclusion bodies and tubular structures in Chenopodium amaranticolor plants infected with strawberry latent ringspot virus. Journal of General Virology 7:47–54
     [Google Scholar]
  18. Roberts K., Northcote D. H. 1971; Ultrastructure of the nuclear envelope; structural aspects of the interphase nucleus of sycamore suspension culture cells. Microscopica acta 71:102–120
     [Google Scholar]
  19. Stubbs L. L. 1948; A new virus disease of carrots; its transmission, host range and control. Australian Journal of Scientific Research, B 1:303–331
     [Google Scholar]
  20. Stubbs L. L. 1952; Further host range and transmission studies with a virus disease of carrots endemic in Australia. Australian Journal of Scientific Research, B 5:399–408
     [Google Scholar]
  21. Van Der Scheer C., Groenewegen J. 1971; Structure in cells of Vigna unguiculata infected with cowpea mosaic virus. Virology 46:493–497
     [Google Scholar]
  22. Watson M. A., Serjeant E. P., Lennon E. A. 1964; Carrot motley dwarf and parsnip mottle viruses. Annals of Applied Biology 54:153–166
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-21-2-269
Loading
Cytopathological Changes and Extractable Infectivity in Nicotiana clevelandii Leaves Infected with Carrot Mottle Virus
J. Gen. Virol. 21, 269 (1973); https://doi.org/10.1099/0022-1317-21-2-269
/content/journal/jgv/10.1099/0022-1317-21-2-269
/content/journal/jgv/10.1099/0022-1317-21-2-269
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error