1887

Abstract

SUMMARY

A clone of baby rabbit kidney cells transformed with SV 40 virus was found to be releasing virus at the rate of about 1 infectious unit per 10 cells per day. Virus antigen was detected in between 1 in 10 and 1 in 10 cells. hybridization with radioactive SV 40 complementary RNA revealed that about 3% of the transformed cells were producing virus DNA. When the transformed cells were fused with permissive cells and incubated in anti-SV 40 serum between 2% and 8% of the heterokaryons formed produced SV 40 virus. A model is proposed according to which the cells in this clone, and possibly other transformed clones, may exist in one of three states: (A) the commonest state, in which virus DNA is not replicable autonomously; (B) a state in which virus DNA can be replicated autonomously, but late virus proteins are not made; and (C) a state in which complete virus can be synthesized.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-1-69
1973-10-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/1/JV0210010069.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-1-69&mimeType=html&fmt=ahah

References

  1. Black P. H., Rowe W. P. 1963; SV 40 induced proliferation of tissue culture cells of rabbit, mouse and porcine origin. Proceedings of the Society for Experimental Biology and Medicine 114:721–727
    [Google Scholar]
  2. Boyd V. A. L., Butel J. S. 1972; Demonstration of infectious deoxyribonucleic acid in transformed cells. 1. Recovery of SV 40 from yielder and non-yielder transformed cells. Journal of Virology 10:399–409
    [Google Scholar]
  3. Dunn A. R., Gallimore P. H., Jones K. W., McDougall J. K. 1973; In situ hybridisation of adenovirus RNA and DNA. II. Detection of adenovirus-specific DNA in transformed and tumour cells. International Journal of Cancer (in the press)
    [Google Scholar]
  4. Gall J. G., Pardue M. L. 1969; Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proceedings of the National Academy of Sciences of the United States of America 63:378–383
    [Google Scholar]
  5. Gerber P. 1963; Tumors induced in hamsters by Simian virus 40: persistent sub viral infection. Science, New York 140:889–890
    [Google Scholar]
  6. Harris H., Watkins J. F. 1965; Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species. Nature, London 205:640–646
    [Google Scholar]
  7. McDougall J. K., Dunn A. R., Jones K. W. 1972; In situ hybridisation of adenovirus RNA and DNA. Nature, London 236:346–348
    [Google Scholar]
  8. Pope J. H., Rowe W. P. 1964; Detection of specific antigen in SV 40-transformed cells by immunofluorescence. The Journal of Experimental Medicine 120:121–128
    [Google Scholar]
  9. Schell K., Maryak J. 1966; Susceptibility of rabbit kidney cells of various passage levels to infection with SV 40 virus. Archiv für die gesamte Virusforschung 19:403–414
    [Google Scholar]
  10. Schell K. 1968; Persistence of SV 40 virus in transformed rabbit kidney cells. Proceedings of the Society for Experimental Biology and Medicine 128:1145–1148
    [Google Scholar]
  11. Watkins J. F. 1970; The effects of some metabolic inhibitors on the ability of SV 40 virus in transformed cells to be detected by cell fusion. Journal of Cell Science 6:721–738
    [Google Scholar]
  12. Watkins J. F. 1972; Fusion of cells for virus studies and production of cell hybrids. In Methods in Virology vol v chap. 1 pp 1–32 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  13. Watkins J. F., Dulbecco R. 1967; Production of SV 40 virus in heterokaryons of transformed and susceptible cells. Proceedings of the National Academy of Sciences of the United States of America 58:1396–1403
    [Google Scholar]
  14. Westphal H., Dulbecco R. 1968; Viral DNA in polyoma- and SV40-transformed cell lines. Proceedings of the National Academy of Sciences of the United States of America 59:1158–1165
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-1-69
Loading
/content/journal/jgv/10.1099/0022-1317-21-1-69
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error