1887

Abstract

SUMMARY

The use of the latex agglutination test to assay iridescent virus (TIV) and to detect its presence in infected insects is described. The effects of varying proportions of virus and antibody-sensitized latex, type of plate, shaking, and incubation temperature were investigated, and the optimum conditions for the reaction of antibody-sensitized latex particles with TIV were determined. Precautions which must be taken to prevent and detect non-specific positive results are described. The sensitivity of the latex test is compared with that of the tube precipitation test and an ID determination in larvae.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-1-181
1973-10-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/1/JV0210010181.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-1-181&mimeType=html&fmt=ahah

References

  1. Aapola A. I. E., Rochow W. F. 1971; Relationships among three isolates of barley yellow dwarf virus. Virology 46:127–141
    [Google Scholar]
  2. Abu salih H. S., Murant A. F., Daft M. J. 1968; The use of antibody-sensitized latex particles to detect plant viruses. Journal of General Virology 3:299–302
    [Google Scholar]
  3. Bercks R. 1967; Methodische Untersuchungen über den serologischen Nachweis pflanzenpathogener Viren mit dem Bentonit - Flockungs-Test, dem Latex-Test und dem Bariumsulfat-Test. Phytopathologische Zeitschrift 58:1–17
    [Google Scholar]
  4. Bercks R., Querfurth G. 1969; Weitere methodische Untersuchungen über den Latex-Test zum serologischen Nachweis pflanzenpathogener Viren. Phytopathologische Zeitschrift 65:243–256
    [Google Scholar]
  5. Bercks R., Querfurth G. 1971; The use of the latex test for the detection of distant serological relationships among plant viruses. Journal of General Virology 12:25–32
    [Google Scholar]
  6. Carter J. B. 1973; The mode of transmission of Tipula iridescent virus. I. Source of infection. Journal of Invertebrate Pathology 21:123–130
    [Google Scholar]
  7. Cunningham J. C., Tinsley T. W. 1968; A serological comparison of some iridescent non-occluded insect viruses. Journal of General Virology 3:1–8
    [Google Scholar]
  8. Day M. F., Mercer E. H. 1964; Properties of an iridescent virus from the beetle Sericesthis pruinosa. Australian Journal of Biological Sciences 17:892–902
    [Google Scholar]
  9. Gerber J. D., Riley R. E., Jones R. D. 1972; Evaluation of a microtiter latex agglutination test for histoplasmosis. Applied Microbiology 24:191–197
    [Google Scholar]
  10. Hukuhara T., Hashimoto Y. 1966; Aggregation of Tipula iridescent virus with polystyrene latex particles. Journal of Invertebrate Pathology 8:540–542
    [Google Scholar]
  11. Mclaughlin R. E., Scott H. A., Bell M. R. 1972; Infection of the boll weevil by Chilo iridescent virus. Journal of Invertebrate Pathology 19:285–290
    [Google Scholar]
  12. Niel G., Gentilini M., Charmot G., Couture J. 1972; Le test au latex applique au diagnostic serologique de l’amibiase: valeur comparee a celle de l’immunofluorescence et de l’immunoelectrophorese. Bulletin de la Societe de Pathologie exotique 65:382–390
    [Google Scholar]
  13. Xeros N. 1954; A second virus disease of the leatherjacket, Tipula paludosa. Nature, London 174:562
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-1-181
Loading
/content/journal/jgv/10.1099/0022-1317-21-1-181
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error