1887

Abstract

SUMMARY

In cultured cells at 28 °C, Semliki Forest virus (SFV, an alphavirus; Wildy, 1971) shows a latent period, growth rate and yield per cell similar to that obtained with the same virus in three vertebrate cell lines at 37 °C. SFV and the flavivirus Kunjin both grow more slowly and to lower titre in cells (Peleg, 1968) than in cells. Kunjin grows more slowly than SFV in both mosquito and vertebrate cell lines. No replication of either virus occurred in another cell line (Grace, 1966).

The effect of adsorption conditions on the percentage of cells infected by SFV is examined. In both mosquito and Vero cells the temperature giving the shortest latent period of SFV growth is close to the optimal temperature for cell growth. No c.p.e. were visible in infected mosquito cells; this contrasts with the invariant destruction of vertebrate cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-2-225
1973-08-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/2/JV0200020225.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-2-225&mimeType=html&fmt=ahah

References

  1. Buckley S. M. 1969; Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses (33940). Proceedings of the Society for Experimental Biology and Medicine 131:25–30
    [Google Scholar]
  2. Dalgarno L., Davey M. W. 1973; Virus Replication. In Viruses and Invertebrates chapter 14 Edited by Gibbs A. J. Amsterdam: North Holland: (in the press.)
    [Google Scholar]
  3. Doi R., Shirasaka A., Sasa M. 1967; The mode of development of Japanese encephalitis virus in the mosquito Culex tritaeniorhynchus summorosus as observed by the fluorescent antibody technique. Japanese Journal of Experimental Medicine 37:227–238
    [Google Scholar]
  4. Grace T. D. C. 1962; Establishment of four strains of cells from insect tissues grown in vitro. Nature, London 195:788–789
    [Google Scholar]
  5. Grace T. D. C. 1966; Establishment of a line of mosquito (Aedes aegypti L.) cells grown in vitro. Nature, London 211:366–367
    [Google Scholar]
  6. Hsu S. H. 1971; Preliminary observations on growth of arboviruses in a newly-established line of mosquito cells (Culex quinquefasciatus SAY). In Current Topics in Microbiology and Immunology vol. 55 pp. 140–148 Edited by Weiss E. New York: Springer-Verlag;
    [Google Scholar]
  7. Janzen H. G., Rhodes A. J., Doane F. W. 1970; Chikungunya virus in salivary glands of Aedes aegypti (L): an electron microscope study. Canadian Journal of Microbiology 16581–586
    [Google Scholar]
  8. Lam K. S. K., Marshall I. D. 1968; Dual infections of Aedes aegypti with arboviruses, II. Salivary gland damage by Semliki Forest Virus in relation to dual infections. American Journal of Tropical Medicine and Hygiene 17:637–644
    [Google Scholar]
  9. La Motte L. C. JUN 1960; Japanese B encephalitis virus in the organs of infected mosquitoes. American Journal of Hygiene 72:73–87
    [Google Scholar]
  10. Mims C. A., Day M. F., Marshall I. D. 1966; Cytopathic effect of Semliki Forest Virus in the mosquito Aedes aegypti. American Journal of Tropical Medicine and Hygiene is:775–784
    [Google Scholar]
  11. Mussgay M. 1964; Growth cycle of arboviruses in vertebrate and arthropod cells. Progress in Medical Virology 6:193–267
    [Google Scholar]
  12. Peleg J. 1968; Growth of arboviruses in monolayers from subcultured mosquito embryo cells. Virology 35:617–619
    [Google Scholar]
  13. Peleg J. 1969; Inapparent persistent virus infection in continuously grown Aedes aegypti mosquito cells. Journal of General Virology 5:463–471
    [Google Scholar]
  14. Porterfield J. S. 1960; A simple plaque inhibition test for the study of arthropod-borne viruses. Bulletin of the World Health Organization 23:373–380
    [Google Scholar]
  15. Rehacek J. 1968; The growth of arboviruses in mosquito cells in vitro. Acta virologica 12:241–246
    [Google Scholar]
  16. Schneider L. 1969; Establishment of three diploid cell lines of Anopheles stephensi (Diptera: Culicidae). Journal of Cell Biology 42:603–606
    [Google Scholar]
  17. Singh K. R. P. 1967; Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Current Science 36:506–508
    [Google Scholar]
  18. Stevens T. M. 1970; Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture (34793). Proceedings of the Society for Experimental Biology and Medicine 134:356–361
    [Google Scholar]
  19. Thomas L. A. 1963; Distribution of the virus of Western equine encephalomyelitis in the mosquito vector, Culex tarsalis. American Journal ‘Hygiene 78:150–165
    [Google Scholar]
  20. Tikasingh E. S., Spence L., Downs W. G. 1966; The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. American Journal of Tropical Medicine & Hygiene 15:219–226
    [Google Scholar]
  21. Whitfield S. G., Murphy F. A., Sudia W. D. 1971; Eastern equine encephalomyelitis virus: an electron microscopic study of Aedes triseriatus (SAY) salivary gland infection. Virology 43:110–122
    [Google Scholar]
  22. Wildy P. 1971; Classification and nomenclature of viruses. In Monographs in Virology vol 5 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-20-2-225
Loading
/content/journal/jgv/10.1099/0022-1317-20-2-225
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error