1887

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Volume 20, Issue 2

Effect of Interferon on Virus Production from Isolated Single Cells * Free

Abstract

SUMMARY

Microdrops were used to isolate interferon treated mouse L cells infected with mengovirus. Three parameters were measured, (1) the time of virus yield, (2) the yield/cell, and (3) the percentage of yielding cells. More than 97% of the control single cells yielded an average of 167 p.f.u./cell with most of them yielding between 11 and 14 h post-infection. Cells treated with interferon yielded later than controls (if at all), and all could be protected to some degree. In both treated and control cells, virus was released over a period of about 15 min. Increasing the multiplicity of infection of challenge virus reduced the effectiveness of a given interferon dose, while increasing the concentration of interferon increased the length of the latent period, decreased the size of the yield, and decreased the number of yielding cells. On the basis of these observations it is proposed that (1) the direct effect of interferon protection is to delay a critical step in virus synthesis, (2) the reduction in yield size and in the number of yielders is due to a progressive loss of the inherent ability of the cell to produce virus, and (3) the delay is the result of interferon, or a molecule induced by it, acting as a competitive inhibitor of some cellular or virus product, needed for the completion of virus synthesis.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1973
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1973-08-01
2024-04-26
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References

  1. Allison A. C., Sandeun K. 1963; Activation of lysosomal enzymes in virus-infected cells and its possible relationship to cytopathic effects. Journal of Experimental Medicine 117:879–887
     [Google Scholar]
  2. Baron S., Buckler C. E., Levy H. B., Friedman R. M. 1967; Some factors affecting the interferon-induced antiviral state. Proceedings of the Society for Experimental Biology and Medicine 125:1320–1326
     [Google Scholar]
  3. Bubel H. C. 1967; Protein leakage from mengovirus-infected cells. Proceedings of the Society for Experimental Biology and Medicine 125:783–786
     [Google Scholar]
  4. Catalano L. W. Jun, Baron S. 1970; Protection against herpes and encephalomyocarditis virus encephalitis with a double-stranded RNA inducer of interferon. Proceedings of the Society for Experimental Biology and Medicine 133:684–687
     [Google Scholar]
  5. Demaeyer E., Schonne E. 1964; Starch gel as an overlay for the plaque assay of animal viruses. Virology 24:13–18
     [Google Scholar]
  6. Dulbecco R. 1952; Production of plaques in monolayer tissue cultures by single particles of an animal virus. Proceedings of the National Academy of Sciences of the United States of America 38:747–752
     [Google Scholar]
  7. Eagle H., Piez K. 1962; The population-dependentrequirement by cultures mammalian cells for metabolites which they can synthesize. Journal of Experimental Medicine 116:29–43
     [Google Scholar]
  8. Fisher H. W., Puck T. T. 1956; On the functions of X-irradiated feeder cells in supporting growth of single mammalian cells. Proceedings of the National Academy of Sciences of the United States of America 42:900–906
     [Google Scholar]
  9. Flanagan J. F. 1965; Hydrolytic enzymes in KB cells infected with polio-virus and Herpes simplex virus. Journal of Bacteriology 91:789–797
     [Google Scholar]
  10. Franklin R. M. 1962; A cytochemical description of the multiplication of Mengo virus in L-929 cells. Journal of Cell Biology 12:1–16
     [Google Scholar]
  11. Gallagher I. G., Khoobyarian N. 1969; Adenovirus susceptibility to interferon: Sensitivity of types 2, 7, and 12 to human interferon. Proceedings of the Society for Experimental Biology and Medicine 130:137–142
     [Google Scholar]
  12. Gandhi S. S., Stewart R. B. 1969; The production and effect of interferon on influenza virus growth in chick embryo lung epithelial and fibroblast cell cultures. Canadian Journal of Microbiology 15:273–277
     [Google Scholar]
  13. Gifford G. E., Ghosh S. N. 1965; Effect of interferon on vaccinia virus yield and plaque formation. Proceedings of the Society for Experimental Biology and Medicine 120:308–310
     [Google Scholar]
  14. Hallum J. V., Thacore H. R., Youngner J. S. 1970; Factors affecting the sensitivity of different viruses to interferon. Journal of Virology 6:156–162
     [Google Scholar]
  15. Hallum J. V., Youngner J. S. 1970; Quantitative aspects of inhibition of virus replication by interferon in chick embryo cell cultures. Journal of Bacteriology 92:1047–1050
     [Google Scholar]
  16. Ho M. 1962; Kinetics considerations of the inhibitory action of an interferon produced in chick cultures infected with Sindbis virus. Virology 17:262–275
     [Google Scholar]
  17. Joklik W. K., Merigan T. C. 1966; Concerning the mechanism of action of interferon. Proceedings of the National Academy of Sciences of the United States of America 65:558–565
     [Google Scholar]
  18. Levine S. 1962; Some characteristics of an interferon derived from embryonated eggs infected with NDV. Virology 17:593–595
     [Google Scholar]
  19. Levine S., Magee W. E., Hamilton R. D., Miller O. V. 1967; Effect of interferon on early enzyme and viral DNA synthesis in Vaccinia virus infection. Virology 32:33–40
     [Google Scholar]
  20. Lockart R. Z. Jun, Horn B. 1963; Interaction of an interferon with L Cells. Journal of Bacteriology 85:996–1002
     [Google Scholar]
  21. Mayer V., Sokol F., Vilcek J. 1962; Infection of interferon treated cells with EEE virus and its RNA. Virology 16:359–362
     [Google Scholar]
  22. Rubin H. 1966; A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells. Experimental Cell Research 41:138–148
     [Google Scholar]
  23. Takahashi K., Okada T. S. 1970; An analysis of the effect of ‘Conditioned medium’ upon the cell culture at low density. Development, Growth, Differentiation 12:65–77
     [Google Scholar]
  24. Takemoto K. K., Baron S. 1966; Non-heritable interferon resistance in a fraction of virus populations. Proceedings of the Society for Experimental Biology and Medicine 121:670–675
     [Google Scholar]
  25. Taylor J. 1965; Studies on the mechanism of action of interferon. Virology 26:340–349
     [Google Scholar]
  26. Wagner R. R. 1961; Biological studies of interferon. I. Suppression of cellular infection with Eastern equine encephalomyelitis virus. Virology 13:323–337
     [Google Scholar]
  27. Wolff D. A., Bubel H. C. 1964; Disposition of lysosomal enzymes as related to specific viral cytopathic effects. Virology 24:502–504
     [Google Scholar]
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Effect of Interferon on Virus Production from Isolated Single Cells*
J. Gen. Virol. 20, 127 (1973); https://doi.org/10.1099/0022-1317-20-2-127
/content/journal/jgv/10.1099/0022-1317-20-2-127
/content/journal/jgv/10.1099/0022-1317-20-2-127
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