1887

Abstract

Summary

This investigation has been focused on the significance of residual growth in the latent period after ultraviolet irradiation of a lysogenic strain of (111). It was shown that the organisms divide once or twice before the development of free phage and the number of infective centres are increased by about 100 % in 30 min. after irradiation.

When celbenin (sodium 6-(2,6 dimethoxy benzamido) penicillinate) was added to the culture after irradiation, the number of infective centres decreased progressively, and no free phage developed. A delay of 10, 20 or 30 min. in introduction of the drug allowed an increasing fraction of the infective centres to develop and to yield free phage.

These observations lead to the suggestion that ultraviolet irradiation inhibits the synthesis of new repressor of vegetative phage development, but has no influence on repressor molecules present beforehand. Residual growth of the irradiated bacteria serves to dilute the intracellular repressor concentration below an effective level.

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1968-05-01
2022-01-28
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References

  1. Adams M. H. 1959 Bacteriophages p 450 New York: Interscience Publishers, Inc;
    [Google Scholar]
  2. Benzer S. 1955; Fine structure of a genetic region in bacteriophage. Proc. natn. Acad. Sci., U.S.A 41:344
    [Google Scholar]
  3. Bertani G. 1958; Lysogeny. In Advances in Virus Research 5 p 151 New York: Academic Press, Inc;
    [Google Scholar]
  4. Braun W. 1965 Bacterial Genetics, 2nd. edition p 265 Philadelphia: W. B. Saunders Company;
    [Google Scholar]
  5. Burnet F. M. 1929; A method for the study of bacteriophage multiplication in broth. Br. J. exp. Path 10:109
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  7. Gardner A. D. 1940; Morphological effects of penicillin on bacteria. Nature; Lond: 146837
    [Google Scholar]
  8. Gradwohl R. B. H. 1956 Clinical laboratory methods and diagnosis, 5th. ed p 1479 St Louis: The Mosby Co;
    [Google Scholar]
  9. Hayes W. 1964 The Genetics of Bacteria and Their Viruses pp 409414 Blackwell Scientific Publications; Oxford:
    [Google Scholar]
  10. Hertman I., Luria S. E. 1967; Transduction studies on the role of rec+ gene in the ultraviolet induction of prophage lambda. J. molec. Biol 23:117
    [Google Scholar]
  11. Jacob F. 1954; Les bactéries lysogènes et la notion des provirus. Masson et Cie; Paris:
    [Google Scholar]
  12. Jacob F., Fuerst C. R. 1958; The mechanism of lysis studied with defective lysogenic bacteria. J. gen. Microbiol 18:518
    [Google Scholar]
  13. Jacob F., Monod J. 1961; Genetic regulatory mechanisms in the synthesis of proteins. J. molec. Biol 3:318
    [Google Scholar]
  14. Jacob F., Wollman E. L. 1953; Induction of phage development in lysogenic bacteria. Cold Spring Harb. Symp. quant. Biol 18:101
    [Google Scholar]
  15. Jacob F., Wollman E. L. 1959; Lysogeny. In The Viruses Burnet F. M., Stanley W. M. Ed. by 2 p 319 New York: Academic Press;
    [Google Scholar]
  16. Lwoff A. 1953; Lysogeny. Bact. Rev 17:269
    [Google Scholar]
  17. Lwoff A., Siminowttch L., Kjeldgaard N. 1950; Induction de la production de bactériophages chez une bactérie lysogne. Annls Inst. Pasteur, Paris 79:815
    [Google Scholar]
  18. Murray R. G. E., Francombe W. H., Mayall B. H. 1959; The effect of penicillin on the structure of staphyloccoccal cell walls. Can. J. Microbiol 5:641
    [Google Scholar]
  19. Price W. H. 1948; Bacteriophage formation without bacterial growth. Formation of Staphylococcus phage in the presence of bacteria inhibited by penicillin. J. gen. Physiol 31:119
    [Google Scholar]
  20. Sadler J. R., Novick A. 1965; The properties of repressor and the kinetics of its action. J. molec. Biol 12:305
    [Google Scholar]
  21. Sompoitnsky D. 1963; Bacteriophage typing of Staphylococcus aureus in Israel. A new 'epidemic' type in hospital infections. Am. J. Hyg 77:54
    [Google Scholar]
  22. Stent G. S. 1963 Molecular Biology of Bacterial Viruses p 324 San Francisco: W. H. Freeman and Co;
    [Google Scholar]
  23. Sussman R., Jacob F. 1962; Sur un système de répression thermosensible chez le bactériophage A d’ Escherichia coli . C. r. hebd. Séanc. Acad. Sci. Paris 254:1517
    [Google Scholar]
  24. Tomizawa J. -I., Ogawa T. 1967a; Effect of ultraviolet irradiation on bacteriophage lambda immunity. J. molec. Biol 23:247
    [Google Scholar]
  25. Tomizawa J. -I., Ogawa T. 1967b; Inhibition of growth of rlI mutants of bacteriophage T4 by immunity substance of bacteriophage lambda. J. molec. Biol 23:277
    [Google Scholar]
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