1887

Abstract

Summary

The passive haemagglutination and bentonite flocculation tests were compared for detecting plant viruses, using antibody-sensitized tanned red cells or bentonite particles. Parsnip yellow fleck, raspberry ringspot, turnip yellow mosaic, narcissus mosaic, potato X and tobacco rattle viruses were detected by both tests in purified preparations and, where studied, in crude plant extracts. The highest antigen titres were obtained only when the red cells or bentonite particles were sensitized with optimal amounts of antibody; these had to be found experimentally because they differed between antisera and between the two tests. In comparative experiments the bentonite flocculation and passive haemagglutination tests were respectively about 2 to 5 and ioo to 125 times more sensitive than tube-precipitin tests for detecting the elongated viruses and about 10 to 20 and 500 to 600 times more sensitive for detecting the isometric viruses. The minimum concentrations of virus detected were 1·8 to 2·6 g./ml. with the bentonite flocculation test and 0·04 to 0·24 g./ml. with the passive haemagglutination test. Red cells treated with formalin before they were tanned and sensitized with antibody could be preserved at − 14° for up to 8 weeks without loss of activity.

In experiments with turnip yellow mosaic virus, extracts from healthy plants of eight species tested had little or no effect on either test but concentrated extracts from and plants caused non-specific agglutination of unformalinized red cells, and dilute extracts from plants decreased eightfold the sensitivity of the passive haemagglutination test. These effects did not occur with for- malinized red cells.

Antibody-sensitized red cells were used in haemagglutination-inhibition tests to determine antiserum titres; antisera to turnip yellow mosaic and raspberry ringspot viruses gave titres 32 times higher than those obtained in tube- precipitin tests.

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1968-01-01
2022-01-21
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References

  1. Bercks R. 1967; Methodische Untersuchungen über den serologischen Nachweis pflanzenpathogener Viren mit dem Bentonit-Flockungstest, dem Latex-Test und dem Bariumsulfat-Test. Phytopath. Z 58:1
    [Google Scholar]
  2. Boyden S. V. 1951; The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J. exp. Med 93:107
    [Google Scholar]
  3. Bozicevich J., Scott H. A., Vincent M. M. 1963; The bentonite flocculation test for detection of plant viruses and titration of antibody. Proc. Soc. exp. Biol. Med 114:750
    [Google Scholar]
  4. Butler W. T. 1963; Hemagglutination studies with formalinized erythrocytes. J. Immun 90:663
    [Google Scholar]
  5. Cannon P. R., Marshall C. E. 1940; An improved serological method for the determination of precipitative titres of antisera. J. Immun 38:365
    [Google Scholar]
  6. Cavelti P. 1947; The technic of collodion particle agglutination. J. Immun 57:141
    [Google Scholar]
  7. Csizmas L. 1960; Preparation of formalinized erythrocytes. Proc. Soc. exp. Biol. Med 103:157
    [Google Scholar]
  8. Cunningham J. C., Tinsley T. W., Walker J. M. 1966; Haemagglutination with plant and insect viruses. J. gen. Microbiol 42:397
    [Google Scholar]
  9. Fleck L., Evenchik Z. 1962; Latex agglutination test with Brucella antigen and antiserum. Nature; Lond: 194548
    [Google Scholar]
  10. Fulthorpe A. J., Rorrr I. M., Doniach D., Couchman K. 1961; A stable sheep cell preparation for detecting thyroglobulin auto-antibodies and its clinical application. J. clin. Path 14:654
    [Google Scholar]
  11. Gilboa-Garber N., Nelken D. 1963; Passive agglutination tests. Nature, Land 197:157
    [Google Scholar]
  12. Ingraham J. S. 1958; The preparation and use of formalinized erythrocytes with attached antigens or haptens to titrate antibodies. Proc. Soc. exp. Biol. Med 99:452
    [Google Scholar]
  13. Jermoljev E., Albrechtova L. 1965; Aplikace suspenze latex V sérologické diagnóze virových chorob rostlin. Ochr. Rost 2:51
    [Google Scholar]
  14. Kahn R. P., Scott H. A., Bozicevich J., Vincent M. M. 1967; Detection of potato viruses X, M and S in dormant potato tubers by the bentonite flocculation test. Phytopathology 57:61
    [Google Scholar]
  15. Loring H. S., Wyckoff R. W. G. 1937; The ultracentrifugal isolation of latent mosaic virus protein. J. biol. Chem 121:225
    [Google Scholar]
  16. Markham R. 1959; The biochemistry of plant viruses. In The Viruses II: p 102 Burnet F. M., Stanley W. M. Ed. Academic Press; New York and London:
    [Google Scholar]
  17. Markham R., Smith K. M. 1949; Studies on the virus of turnip yellow mosaic. Parasitology 39:330
    [Google Scholar]
  18. Murant A. F., Goold R. A. 1966; Parsnip viruses. Rep. Scottish Hort. Res. Inst for 1964 and 1965 p 50
    [Google Scholar]
  19. Murant A. F., Goold R. A. 1967; Parsnip viruses. Rep. Scottish Hort. Res. Inst for 1966 p 65
    [Google Scholar]
  20. Reichmann M. E. 1959; Potato X virus. II. Preparation and properties of purified non-aggregated virus from tobacco. Can. J. Chem 37:4
    [Google Scholar]
  21. Saito Y., Iwata Y. 1964; Hemagglutination test for titration of plant virus. Virology 22:426
    [Google Scholar]
  22. Saito Y., Takanashi K., Iwata Y. 1964; Studies on the soil-borne virus diseases of wheat and barley. II. Detection of virus by hemagglutination test. Bull. natn. Inst, agric. Sci., Tokyo 17:23
    [Google Scholar]
  23. Scott H. A., Kahn R. P., Bozicevich J., Vincent M. M. 1964; Detection of potato virus X in tubers by the bentonite flocculation test. Phytopathology 54:1292
    [Google Scholar]
  24. Singer J. M., Plotz C. M. 1956; The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am. J. Med 21:888
    [Google Scholar]
  25. Stavitsky A. B. 1954; Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination-inhibition reactions with tannic acid and protein- treated red blood cells. J. Immun 72:360
    [Google Scholar]
  26. Stavitsky A. B. 1964; Haemagglutination and haemagglutination-inhibition reactions with tannic acid- and bis-diazotised benzidine-protein-conjugated erythrocytes. In Immunological Methods p 363 Ackroyd A. F. Ed Oxford: Blackwell;
    [Google Scholar]
  27. Steere R. L. 1956; Purification and properties of tobacco ringspot virus. Phytopathology 46:60
    [Google Scholar]
  28. Tomlinson J. A., Walkey D. G. A., Hughes D. E., Watson D. H. 1965; Multiple transverse breakage of the filamentous particles of turnip mosaic virus by ultrasonic vibration. Nature; Lond: 207495
    [Google Scholar]
  29. Wolff S. M., Ward S. B., Landy M. 1963; Serologic properties of bentonite particles coated with microbial polysaccharides. Proc. Soc. exp. Biol. Med 114:530
    [Google Scholar]
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