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Baby hamster kidney cells were infected with herpes simplex virus under one-step growth conditions. Virus-specific antigens were traced by ‘direct’ and ‘indirect’ immunofluorescence and their times of appearance estimated by agar-gel diffusion using an antiserum made by immunization of rabbits with herpes-virus-infected rabbit cells. Twelve distinct precipitin lines were detected by agar-gel diffusion and a succession of fluorescent patterns was distinguished. Quantitative estimations indicated progressive accumulation of antigen in various patterns within the nucleus, in the cytoplasm and at the cell membrane. These patterns were correlated with the cellular distortions seen with a conventional cytological technique.
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