1887

Abstract

SUMMARY

Dithiothreitol (DTT), a reagent which cleaves disulphide bonds, destroyed the haemagglutinin, neuraminidase and ‘haemolysin’ of Sendai virus. The treated virus particles appeared morphologically intact. They failed to bind haemagglutination-inhibiting (HI) and neuraminidase-inhibiting (NI) antibodies. Antisera against DTT-treated virus inhibited haemolysis induced by intact virus, although they did not contain detectable HI, NI or virus-neutralizing antibodies. Treated and untreated virus particles were labelled with [C]-iodoacetamide or with [C]--ethylmaleimide, disrupted with SDS and subsequently electrophoresed in polyacrylamide gels. Thus, polypeptide species with accessible cysteinyl residues, either originally present in intact virus or resulting from reduction of disulphide bonds, were distinguished. The significance of these findings is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-19-1-9
1973-04-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/19/1/JV0190010009.html?itemId=/content/journal/jgv/10.1099/0022-1317-19-1-9&mimeType=html&fmt=ahah

References

  1. Cleland W. W. 1964; Dithiothreitol, a new protective reagent for SH groups. Biochemistry 3:480–482
    [Google Scholar]
  2. Content J., Duesberg P. H. 1970; Electrophoretic distribution of the proteins and glycoproteins of influenza and Sendai virus. Journal of Virology 6:707–716
    [Google Scholar]
  3. Duesberg P. H., Robinson W. S. 1965; Isolation of the nucleic acid of Newcastle Disease Virus (NDV). Proceedings of the National Academy of Sciences of the United States of America 54:794–800
    [Google Scholar]
  4. Heitmann P. 1968; A model for sulfhydryl groups in proteins. Hydrophobic interactions of the cysteine side chain in micelles. European Journal of Biochemistry 3:346–350
    [Google Scholar]
  5. Hosaka Y., Kitano H., Ikeguchi S. 1966; Studies on the pleomorphism of HVJ virions. Virology 29:205–221
    [Google Scholar]
  6. Kato N. 1967; Nonhemagglutinating complement-fixing V antigen of parainfluenza I virus (HVJ). II.The properties of nonhemagglutinating V antigen. Virology 31:37–45
    [Google Scholar]
  7. Kosower N. S., Song K.R., Kosower E. M. 1969; Glutathion I. The methyl phenyldiazenecarboxylate (azoester) procedure for intracellular oxidation. Biochimica et biophysica acta 192:1–7
    [Google Scholar]
  8. Laver W. G. 1971; Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  9. Lazdins I., Haslam E. A., White D. O. 1972; The polypeptides of influenza virus. VI. Composition of the neuraminidase. Virology 49:758–765
    [Google Scholar]
  10. Litman G. W., Good R. A., Frommel D., Rosenber G A. 1970; Conformational significance of the intrachain disulfide linkages in immunoglobulins. Proceedings of the National Academy of Sciences of the United States of America 67:1085–1092
    [Google Scholar]
  11. Moore P. B. 1971; Reaction of N-ethylmaleimide with the ribosomes of Escherichia coli. Journal of Molecular Biology 60:169–184
    [Google Scholar]
  12. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of Simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 6:677–684
    [Google Scholar]
  13. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of Simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  14. Neurath A. R. 1963; Effect of trypsin treatment on the haemolytic, haemagglutinating, neuraminidase and adenosine diphosphatase activities of Sendai virus. Acta virologica 7:490–497
    [Google Scholar]
  15. Neurath A. R., Ruben F. L., Rubin B. A., Hartzell R. W., Wong S. 1971; Antibody response in humans to neuraminidase of A2/aichi/68 and B/mass/66 influenza viruses. Proceedings of the Society for Experimental Biology and Medicine 138:47–51
    [Google Scholar]
  16. Neurath A. R., Vernon S. K., Dobkin M. B., Rubin B. A. 1972a; Characterization of subviral components resulting from treatment of rabies virus with tri(n-butyl)phosphate. Journal of General Virology 14:33–48
    [Google Scholar]
  17. Neurath A. R., Vernon S. K., Hartzell R. W., Rubin B. A. 1972b; Haemolysis by Sendai virus: lack of requirement for neuraminidase. Journal of General Virology 16:245–249
    [Google Scholar]
  18. Perper R. J., Okimoto J. T., Cochrum K. C., Ramsey N., Najarian J. S. 1967; A rapid method for purification of large quantities of anti-lymphocytic serum. Proceedings of the Society for Experimental Biology and Medicine 125:575–580
    [Google Scholar]
  19. Rafelson M. E. Jun, Schneir M., Wilson V. W. Jun 1966; Neuraminidases. In The Amino Sugars: The Chemistry and Biology of Compounds Containing Amino Sugars vol 2B chapter 43 pp. 171–179 Edited by Jeanlosz R. W., Balazs E. A. Academic Press: New York;
    [Google Scholar]
  20. Shimizu K., Hosaka Y., Shimizu Y. K. 1972; Solubilization of envelopes of HVJ (Sendai virus) with alkali-Emasol treatment and reassembly of envelope particles with removal of the detergent. Journal of Virology 9:842–850
    [Google Scholar]
  21. Stanley P., Haslam E. A. 1971; The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques. Virology 46:764–773
    [Google Scholar]
  22. Steck T. L. 1972; Cross-linking the major proteins of the isolated erythrocyte membrane. Journal of Molecular Biology 66:295–305
    [Google Scholar]
  23. Thompson K., Harris M., Benjamini E., Mitchell G., Noble M. 1972; Cellular and humoral immunity: a distinction in antigenic recognition. Nature New Biology 238:20–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-19-1-9
Loading
/content/journal/jgv/10.1099/0022-1317-19-1-9
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error