Nine 5-bromodeoxyuridine-induced temperature-sensitive () mutants were isolated from stocks of a syncytial plaque type mutant () itself derived from the non-syncytial wild-type ( ) of the strain 17 of herpes simplex virus type 1. The nine mutants have been assigned to eight cistrons on the basis of different complementation tests.

Non-syncytial revertants isolated from the original mutants were used in reciprocal three-factor crosses of the type: and × . With recombination data from these crosses a linkage map has been constructed. The provisional map which locates nine cistrons is linear and spans about 25 recombination units.

The regular occurrence of plaques with mixed morphology in the progeny from crosses is reported.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error