1887

Abstract

SUMMARY

Nine 5-bromodeoxyuridine-induced temperature-sensitive () mutants were isolated from stocks of a syncytial plaque type mutant () itself derived from the non-syncytial wild-type ( ) of the strain 17 of herpes simplex virus type 1. The nine mutants have been assigned to eight cistrons on the basis of different complementation tests.

Non-syncytial revertants isolated from the original mutants were used in reciprocal three-factor crosses of the type: and × . With recombination data from these crosses a linkage map has been constructed. The provisional map which locates nine cistrons is linear and spans about 25 recombination units.

The regular occurrence of plaques with mixed morphology in the progeny from crosses is reported.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-18-3-329
1973-03-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/18/3/JV0180030329.html?itemId=/content/journal/jgv/10.1099/0022-1317-18-3-329&mimeType=html&fmt=ahah

References

  1. Burge B. W., Pfefferkorn E. R. 1966; Complementation between temperature-sensitive mutants of Sindbis virus. Virology 30:214–223
    [Google Scholar]
  2. Calendar R. 1970; The regulation of phage development. Annual Review of Microbiology 24:241–296
    [Google Scholar]
  3. Campbell A. 1961; Sensitive mutants of bacteriophage A. Virology 14:22–32
    [Google Scholar]
  4. Cooper P. D. 1965; Rescue of one phenotype in mixed infections with heat-defective mutants of type 1 poliovirus. Virology 25:431–438
    [Google Scholar]
  5. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584–596
    [Google Scholar]
  6. Cooper P. D., Geissler E., Scotti P. D., Tannock G. A. 1971 Strategy of the Viral Genome pp. 75100 Edited by Wolstenholme G. E. W., O’Connor M. London: Churchill;
    [Google Scholar]
  7. Dahlberg J. E., Simon E. H. 1968; Complementation in Newcastle disease virus. Bacteriological Proceedings p. 162
    [Google Scholar]
  8. Edgar R. S., Lielausis I. 1964; Temperature-sensitive mutants of bacteriophage T4D: their isolation and genetic characterisation. Genetics 49:649–662
    [Google Scholar]
  9. Epstein W., Beckwith J. R. 1968; Regulation of gene expression. Annual Review of Biochemistry 37:411–436
    [Google Scholar]
  10. Epstein R. H., Bolle A., Steinberg C. M., Kellenberger E., Boy De La Tour E., Chevalley R., Edgar R. S., Susman M., Denhardt G. H., Leilausis A. 1963; Physiological studies of conditional lethal mutations of bacteriophage T4D. Cold Spring Harbor Symposia on Quantitative Biology 28:375–394
    [Google Scholar]
  11. Fenner F. 1970; The genetics of animal viruses. Annual Review of Microbiology 24:297–334
    [Google Scholar]
  12. Fenner F., Sambrook J. F. 1966; Conditional lethal mutants of rabbitpox virus. II. Mutants (p) which fail to multiply in Pk-2a cells. Virology 28:600–609
    [Google Scholar]
  13. Fields B. N., Joklik W. K. 1969; Isolation and preliminary genetic and biochemical characterisation of temperature-sensitive mutants of reovirus. Virology 37:335–342
    [Google Scholar]
  14. Garen A. 1968; Sense and nonsense in the genetic code. Science, New York 160:149–159
    [Google Scholar]
  15. Hay I., Perera P. A. J., Morrison J. M., Gentry G. A., Subak-Sharpe J. H. 1971 Strategy of the Viral Genome355–376 Edited by Wolstenholme G. E. W., O’Connor M. London: Churchill;
    [Google Scholar]
  16. Hershey A. D., Chase M. 1951; Genetic recombination and heterozyzosis in bacteriophage. Cold Spring Harbor Symposia on Quantitative Biology 16:471–479
    [Google Scholar]
  17. House W., Wildy P. 1965; Large scale production of tissue cells and viruses. Laboratory Practice 14:594–595
    [Google Scholar]
  18. Mackenzie J. S. 1970; Isolation of temperature-sensitive mutants and the construction of a preliminary genetic map for influenza virus. Journal of General Virology 6:63–75
    [Google Scholar]
  19. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones. An investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  20. Padgett B. L., Tomkins J. K. N. 1968; Conditional lethal mutants of rabbitpox virus. III. Temperature-sensitive (ts) mutants physiological properties, complementation and recombination. Virology 36:161–167
    [Google Scholar]
  21. Pringle C. R. 1968; Recombination between conditional mutants within a strain of foot-and-mouth disease virus. Journal of General Virology 2:199–202
    [Google Scholar]
  22. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine and ethyl methane sulfonate. Journal of Virology 5:559–567
    [Google Scholar]
  23. Pringle C. R., Duncan I. B., Stevenson M. 1971; Isolation and characterisation of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. Journal of Virology 8:836–841
    [Google Scholar]
  24. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  25. Simpson R. W., Hirst G. K. 1968; Temperature-sensitive mutants of influenza A virus. Isolation of mutants and preliminary observations on genetic recombination and complementation. Virology 35:41–49
    [Google Scholar]
  26. Smith J. D., Barnett L., Brenner S., Russell R. L. 1970; More mutant tyrosine transfer ribonucleic acids. Journal of Molecular Biology 54:1–14
    [Google Scholar]
  27. Subak-Sharpe J. H. 1969 Proceedings of the First International Congress for Virology, Helsinki, 1968 p. 252 Edited by Melnick J. L. Basel: Karger;
    [Google Scholar]
  28. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
  29. Visconti N., Delbruck M. 1953; The mechanism of genetic recombination in phage. Genetics 38:5–33
    [Google Scholar]
  30. Wildy P. 1955; Recombination with herpes simplex virus. Journal of General Microbiology 13:34–36
    [Google Scholar]
  31. Williams J. F., Ustacelebi S. 1971; Complementation and recombination with temperature-sensitive mutants of adenovirus type 5. Journal of General Virology 13:345–348
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-18-3-329
Loading
/content/journal/jgv/10.1099/0022-1317-18-3-329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error