1887

Abstract

SUMMARY

Treatment of purified Newcastle disease virus (NDV) with Triton N101 to activate the virus particle RNA polymerase in the assay removed the glycoproteins and most of the phospholipid from the virus. The subvirus particles produced by the action of Triton N101 on virus had RNA polymerase activity, whereas purified NDV nucleocapsids did not. Apparently, either nucleocapsids did not contain the proteins essential for RNA polymerase activity, or the enzyme was denatured during the preparation of nucleocapsids. Incubation of several purified strains of NDV at 4 °C led to loss of polymerase activity which was regained on incubation at 37 °C. It is suggested that internal configurational changes in the virus particle were the most likely causes of these changes in enzyme activity on incubation at 4 °C and 37 °C.

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1973-03-01
2021-10-17
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References

  1. Barry R. D., Bukrinskaya A. G. 1968; The nucleic acid of Sendai virus and ribonucleic acid synthesis in cells infected by Sendai virus. Journal of General Virology 2:71–79
    [Google Scholar]
  2. Bikel I., Duesberg P. H. 1969; Proteins of Newcastle disease virus and of the viral nucleocapsid. Journal of Virology 4:388–393
    [Google Scholar]
  3. Carraway K. L., Lam A., Kobylka D., Higgings J. 1972; Anomolous staining of membrane lipids on acrylamide gels. Analytical Biochemistry 45:325–331
    [Google Scholar]
  4. De The G., O’Connor T. E. 1966; Structure of a murine leukemia virus after disruption with Tween-ether and comparison with two myxoviruses. Virology 28:713–728
    [Google Scholar]
  5. Evans M. J., Kingsbury D. W. 1969; Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis. Virology 37:597–604
    [Google Scholar]
  6. Haslam E. A., Cheyne I. M., White D. O. 1969; The structural proteins of Newcastle disease virus. Virology 39:118–129
    [Google Scholar]
  7. Hoyle L., Almeida J. D. 1971; The chemical reactions of the haemagglutinins and neuraminidases of different strains of influenza viruses. III. Effects of proteolytic enzymes. Journal of Hygiene, Cambridge 69:461–469
    [Google Scholar]
  8. Huang A. S., Baltimore D., Bratt M. A. 1971; Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. Journal of Virology 7:389–394
    [Google Scholar]
  9. Iinuma M., Yoshida T., Nagai Y., Maeno K., Matsumoto T., Hoshino M. 1971; Subunits of NDV haemagglutinin and neuraminidase subunits of Newcastle disease virus. Virology 46:663–677
    [Google Scholar]
  10. Kobylka D., Khettry A., Shin B. C., Carraway K. L. 1972; Proteins and glycoproteins of the erythrocyte membrane. Archives of Biochemistry and Biophysics 148:475–487
    [Google Scholar]
  11. Kohno S., Kohase M., Shimizu Y. 1969; Studies on interferon induction by Newcastle disease virus (NDV). II. Interferon induction by heat-and acid-treated NDV. Archiv fur die gesamte Virusforschung 28:188–196
    [Google Scholar]
  12. Lomniczi B. 1970; Systemic induction of interferon in chicks with various NDV strains. I. Relationship between virulence of the virus and the mechanism of interferon formation. Archiv fur die gesamte Virusforschung 30:159–166
    [Google Scholar]
  13. Lomniczi B., Burke D. C. 1971; Interferon production by NDV strains of different virulence. Proceedings of the Microbiological Research Group of the Hungarian Academy of Sciences 4:9–14
    [Google Scholar]
  14. Lomniczi B., Meager A., Burke D. C. 1971; Virus RNA and protein synthesis in cells infected with ditferent strains of Newcastle disease virus. Journal of General Virology 13:111–120
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Maizel J. V. Jun., Summers D. F., Scharff M. D. 1970; SDS acrylamide gel electrophoresis and its application to the proteins of poliovirus - and adenovirus - infected human cells. Journal of Cellular Physiology 76:273–288
    [Google Scholar]
  17. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes; application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  18. Meager A., Burke D. C. 1972; Production of interferon by ultraviolet inactivated Newcastle disease virus. Nature, London 235:280–282
    [Google Scholar]
  19. Morser J., Kennedy S. I. T., Burke D. C. 1973; Virus specified proteins in cells infected with Semliki Forest virus. Journal of General Virology (submitted for publication)
    [Google Scholar]
  20. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai Virus. Journal of Virology 6:677–684
    [Google Scholar]
  21. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses; a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  22. Oyama V. I., Eagle H. 1956; Measurement of cell growth in tissue culture with a phenol reagent (Folin-Ciocalteau). Proceedings of the Society for Experimental Biology and Medicine 91:305–307
    [Google Scholar]
  23. Sheaff E. T., Meager A., Burke D. C. 1972; Factors involved in the production of interferon by inactivated Newcastle disease virus. Journal of General Virology 17:163–175
    [Google Scholar]
  24. Walters S., Burke D. C., Skehel J. J. 1967; Interferon production and RNA inhibitors. Journal of General Virology 1:349–362
    [Google Scholar]
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