1887

Abstract

SUMMARY

Upon treatment with nitrous acid, southern bean mosaic virus () was inactivated twice as fast as its isolated . Nitrous acid treatment of resulted in appreciable binding of the virus protein to the nucleic acid. The extent of deamination of aminoribonucleotides, of intraparticle and isolated by nitrous acid was identical. and were inactivated at the same rate following exposure to u.v. light. Furthermore, the extent of photoreactivation of the u.v.-irradiated and was similar. U.v. irradiation did not induce any detectable binding of virus protein to in .

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1973-01-01
2022-08-16
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References

  1. Bawden F. C., Kleczkowski A. 1955; Studies on the ability of light to counteract the inactivating action ultraviolet radiation on plant viruses. Journal of General Microbiology 13:370–382
    [Google Scholar]
  2. Boeye A. 1959; Induction of a mutation in poliovirus. Virology 9:691–700
    [Google Scholar]
  3. Brakke M. K., Van pelt N. 1970; Properties of infectious ribonucleic acid from wheat streak mosaic virus. Virology 42:699–706
    [Google Scholar]
  4. Diener T. O. 1965; Isolation of infectious ribonucleic acid from southern bean mosaic virus. Virology 27:425–428
    [Google Scholar]
  5. Fraenkel-conrat H., Singer B., Tsugita A. 1961; Purification of viral RNA by means of bentonite. Virology 14:54–58
    [Google Scholar]
  6. Furuse K., Watanabe I. 1971; Effects of ultraviolet (UV) irradiation on RNA phages in H2O and D2O. Virology 46:171–172
    [Google Scholar]
  7. Geiduschek E. P. 1961; ‘Reversible’ DNA. Proceedings of the National Academy of Sciences of the United States of America 47:950–955
    [Google Scholar]
  8. Ghabrial S. A., Shepherd R. J., Grogan R. G. 1967; Chemical properties of three strains of southern bean mosaic virus. Virology 33:17–25
    [Google Scholar]
  9. Goddard J., Streeter D., Weber C., Gordon M. P. 1966; Studies on the inactivation of tobacco mosaic virus by ultraviolet light. Photochemistry and Photobiology 5:213–222
    [Google Scholar]
  10. Kaper J. M., Geelen J. L. M. C. 1971; Studies on the stabilizing forces of simple RNA viruses. II. Stability, dissociation and reassembly of cucumber mosaic virus. Journal of Molecular Biology 56:277–294
    [Google Scholar]
  11. Kassanis B., Kleczkowski A. 1965; Inactivation of a strain of tobacco necrosis virus and of the RNA isolated from it, by ultraviolet radiation of different wave lengths. Photochemistry and Photobiology 4:209–214
    [Google Scholar]
  12. Lesnaw J. A., Reichmann M. E. 1969; The structure of tobacco necrosis virus. I. The protein subunit and the nature of nucleic acid. Virology 30:729–737
    [Google Scholar]
  13. Mundry K. W., Gierer A. 1958; Die Erzeugung von Mutationen des Tabakmosaikvirus durch chemische Behandlung seiner Nucleinsäure in vitro . Zeitschrift fur Vererbungslehre 89:614–630
    [Google Scholar]
  14. Price W. C. 1965; Inactivation of southern bean mosaic virus by ultraviolet light. Virology 25:1–8
    [Google Scholar]
  15. Schuster H., Wilhelm R. C. 1963; Reaction differences between tobacco mosaic virus and its free nucleic acid with nitrous acid.. Biochimica et biophysica acta 68:554–560
    [Google Scholar]
  16. Sehgal O. P., Krause G. F. 1968; Efficiency of nitrous acid as an inactivating and mutagenic agent for intact tobacco mosaic virus and its isolated nucleic acid. Journal of Virology 2:966–971
    [Google Scholar]
  17. Sehgal O. P., Soong M. M. 1972; Reaction of nitrous acid with viral nucleic acids in situ. Virology 47:239–243
    [Google Scholar]
  18. Shatkin A. J. 1969 Colorimetric reactions for DNA, RNA and protein determinations. In Fundamental Techniques in Virology231–237 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  19. Siegel A. 1965; Artificial production of mutants of tobacco mosaic virus. Advances in Virus Research 11:25–60
    [Google Scholar]
  20. Siegel A., Wildman S. G., Ginoza W. 1956; Sensitivity of ultraviolet light of infectious tobacco mosaic virus nucleic acid. Nature, London 178:1117–1118
    [Google Scholar]
  21. Streeter D. G., Gordon M. P. 1968; Studies on the role of the coat protein in the U.V. inactivation of U (1) and U (2) strains of TMV . Photochemistry and Photobiology 8:81–92
    [Google Scholar]
  22. Tikochonenko I. 1969; Conformation of viral nucleic acids in situ . Advances in Virus Research 15:201–290
    [Google Scholar]
  23. Weinbraub M., Ragetli H. W. J. 1970; Identification of the constituents of southern bean mosaic virus in crystals of infected cells, and their distribution within the virion. Virology 41:729–239
    [Google Scholar]
  24. Wells J. M., Sisler H. D. 1969; The effect of EDTA and Mg2+ on the infectivity and structure of southern bean mosaic virus. Virology 37:227–236
    [Google Scholar]
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