1887

Abstract

SUMMARY

In cells infected with paramyxovirus progeny 50 S RNA seems to be incorporated immediately into the nucleocapsid (Blair & Robinson, 1970). There has been no report of any attempt to estimate the time interval between the synthesis of a molecule of nucleocapsid protein and its incorporation into the structure of the nucleocapsid. In the experiments described in this paper the problem has been approached with the use of a pulse-chase, using protein precursors. The increase of label in the 200 S peak under chase conditions was used as a measure of the rate of nucleocapsid formation.

Stocks of the strain of Newcastle disease virus (NDV) were grown in eggs. Primary chick embryo cell cultures were inoculated as described by Kingsbury (1966), at a multiplicity of 10 to 20 p.f.u./cell and incubated at 37 °C. Lactalbumin hydrolysate (0.5%) in Hanks’s balanced salt solution (BSS) was used as maintenance medium.

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1972-12-01
2022-09-25
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References

  1. Baltimore D., Huang A. S. 1968; Isopicnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science, New York 162:572–574
    [Google Scholar]
  2. Blair C. D., Robinson W. S. 1970; Replication of Sendai virus. II. Steps in virus assembly. Journal of Virology 5:639–650
    [Google Scholar]
  3. Compans R. W., Choppin P. W. 1967; Isolation and properties of the helical nucleocapsid of the parainfluenza virus SV 5. Proceedings of the National Academy of Sciences of the United States of America 57:949–956
    [Google Scholar]
  4. Hosaka I. 1968; Isolation and structure of nucleocapsid of HVJ. Virology 35:445–457
    [Google Scholar]
  5. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. Journal of Physical Chemistry 65:1138–1145
    [Google Scholar]
  6. Iinuma M., Nagai I., Maeno K., Yoshida T., Matsumoto T. 1971; Studies on the assembly of Newcastle disease virus. Incorporation of structural components into virus particles. Journal of General Virology 12:239–247
    [Google Scholar]
  7. Kaverin N. V., Varich N. L. 1971; Virus-specific RNA formed in Newcastle disease virus-infected cells after suppression of protein synthesis by cycloheximide. Archie fitr die gesamte Virusforschung 35:378–384
    [Google Scholar]
  8. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. Journal of Molecular Biology 18:204–214
    [Google Scholar]
  9. Robinson W. S. 1971; Sendai virus RNA synthesis and nucleocapsid formation in the presence of cycloheximide. Virology 44:494–502
    [Google Scholar]
  10. Spirin A. S., Belitsina N. V., Lerman M. I. 1965; Use of formaldehyde fixation for studies of ribonucleo-protein particles by caesium chloride density gradient centrifugation. Journal of Molecular Biology 14:611–615
    [Google Scholar]
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