1887

Abstract

SUMMARY

Antisera produced in rabbits against extracts of cells infected with type 1 or type 2 herpes simplex virus neutralized thymidine kinase activities of six type 1 and six type 2 herpes virus strains. There was intertypic neutralizing activity, suggesting some common antigenic determinants, but much greater intratypic activity, suggesting that the enzyme bears type-specific determinants.

The effects of some anti-type 1 sera were complicated since they also stabilized the type 2 enzyme activity.

When anti-type 1 and anti-type 2 sera were absorbed with extracts of cells infected with thymidine-kinase deficient mutants of homologous type they retained their power to neutralize thymidine kinase activity but completely lost their ability to neutralize infectivity. Each absorbed serum gave a single precipitin line against the parent strain and none against the parent of the other type. It was surmised that these precipitin lines were associated with type-specific thymidine kinase antigens.

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1972-12-01
2021-10-25
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References

  1. Beale A. J., Christofinis O. C., Furminger I. G. S. 1963; Rabbit cells susceptible to rubella virus. Lancet ii:640–641
    [Google Scholar]
  2. Buchan A., Luff S., Wallis C. 1970; Failure to demonstrate the interaction of subunits of thymidine kinase in cells simultaneously infected with herpes virus and a kinaseiess mutant. Journal of General Virology 9:239–242
    [Google Scholar]
  3. Buchan A., Watson D. H. 1969; The immunological specificity of thymidine kinases in cells infected by viruses of the herpes group. Journal of General Virology 4:461–463
    [Google Scholar]
  4. Buchan A., Watson D. H., Dubbs D. R., Kit S. 1970; Serological study of a mutant of herpes virus unable to stimulate thymidine kinase. Journal of Virology 5:817–818
    [Google Scholar]
  5. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex deficient in thymidine kinase-inducing ability. Virology 22:493–502
    [Google Scholar]
  6. Figueroa M. E., Rawls W. E. 1969; Biological markers for differentiation of herpes virus strains of oral and genital origin. Journal of General Virology 4:259–267
    [Google Scholar]
  7. Goodheart C. R., Plummer G., Waner J. L. 1968; Density differences of DNA of human herpes simplex viruses, types 1 and 2. Virology 35:473–475
    [Google Scholar]
  8. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus-specific thymidine kinase in BHK 21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  9. Lowry S. P., Bresnick E., Rawls W. E. 1971; Differences in thymidine kinase-inducing ability of herpes virus types 1 and 2. Virology 46:958–961
    [Google Scholar]
  10. Nahmias A. J., Dowdle W. R. 1968; Antigenic and biologic differences in herpesvirus hominis. Progress in Medical Virology 10:110–159
    [Google Scholar]
  11. Ogino T., Rapp F. 1971; Differences in thermal stability of deoxythymidine kinase activity in extracts from cells infected with herpes simplex virus type 1 or 2. Virology 46:953–955
    [Google Scholar]
  12. Parker J. D. J., Banatvala J. E. 1967; Herpes genitalis - clinical and virological studies. British Journal of Venereal Diseases 43:212–216
    [Google Scholar]
  13. Plummer G. 1964; Serological comparison of the herpes viruses. British Journal of Experimental Pathology 45:135–141
    [Google Scholar]
  14. Ratcliffe H. 1971; The differentiation of herpes simplex virus type 1 and type 2 by temperature markers. Journal of General Virology 13:181–183
    [Google Scholar]
  15. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  16. Thouless M. E., Skinner G. R. B. 1971; Differences in the properties of thymidine kinase produced in cells infected with type I and type 2 herpes virus. Journal of General Virology 12:195–197
    [Google Scholar]
  17. Vantsis J. T., Wildy P. 1962; Interaction of herpes virus and HeLa cells: comparison of cell killing and infective center formation. Virology 17:225–232
    [Google Scholar]
  18. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology ii:399–408
    [Google Scholar]
  19. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
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