1887

Abstract

SUMMARY

The small-plaque effect induced by exposure of herpes simplex virus to u.v. light was investigated. New results are presented showing that the effect was due to a delay of multiplication of virus in the cells initiating plaques. The delay occurred after entry of virus into the cell and before the replication of virus. The ultimate cause of the delay was probably the formation of thymine dimers, since the small-plaque effect was reversed by photoreactivation in avian cells. Evidence was obtained for co-operative reactivation and host-cell reactivation in infected BHK cells but neither of these phenomena could be related to the small-plaque effect.

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1972-09-01
2021-10-23
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References

  1. ARLETT C. F. 1969; The influence of metabolic inhibitors on the response of Chinese hamster cells to ultraviolet light. Annali deWIstituto Superiore di Sanità 5:367
    [Google Scholar]
  2. CLEAVER J. E. 1968; Defective repair replication of DNA in Xeroderma pigmentosum. Nature, London 218:652
    [Google Scholar]
  3. CLEAVER J. E. 1969; Repair of mammalian cell DNA: effects of drugs and mutations. Annali deWIstituto Superiore di Sanità 5:360
    [Google Scholar]
  4. DULBECCO R., VOGT M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167
    [Google Scholar]
  5. HARM W. 1965; Host-cell reactivation of non-lethal ultraviolet effects. Photochemistry and Photobiology 4:575
    [Google Scholar]
  6. HARM W. 1966; The role of host-cell repair in liquid-holding recovery of u.v.-irradiated. Escherichia coli. Photochemistry and Photobiology 5:747
    [Google Scholar]
  7. HATCHARD C. G., PARKER C. A. 1956; A new sensitive chemical actinometer. II. Potassium ferrioxalate as a standard chemical actinometer. Proceedings of the Royal Society of London, Series A 235:518
    [Google Scholar]
  8. UNDENMANN J., GIFFORD G. E. 1963; Studies on vaccinia virus plaque formation and its inhibition by interferon. I. Dynamics of plaque formation by vaccinia virus. Virology 19:283
    [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  10. LYTLE C. D. 1971; Host-cell reactivation in mammalian cells. I. Survival of ultra-violet-irradiated herpes virus in different cell-lines. Internationaljournal of Radiation Biology 19:329
    [Google Scholar]
  11. MacPherson I., STOKER M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  12. PAUL J. 1961 Cell and Tissue Culture197, 2nd edition. Edinburgh: E. & S. Livingstone, Ltd;
    [Google Scholar]
  13. PFEFFERKORN E. R., BURGE B. W., COADY H. M. 1966; Characteristics of the photoreactivation of pseudo-rabies virus. Journal of Bacteriology 92:856
    [Google Scholar]
  14. PFEFFERKORN E. R., COADY H. M. 1968; Mechanism of photoreactivation of Pseudorabies virus. Journal of Virology 2:474
    [Google Scholar]
  15. PFEFFERKORN E. R., RUTSTEIN C., BURGE B. W. 1965; Photoreactivation of Pseudorabies virus. Virology 27:457
    [Google Scholar]
  16. RABSON A. S., TYRRELL S. A., LEGALLIS F. Y. 1969; Growth of ultra violet-damaged herpesvirus in Xeroderma pigmentosum. Proceedings of the Society for Experimental Biology and Medicine 132:802
    [Google Scholar]
  17. REGAN J. D. 1969; On the two mechanisms of repair of ultraviolet-damaged DNA in vertebrate cells. Annali deiristituto Superiore di Sanità 5:355
    [Google Scholar]
  18. REGAN J. D., SETLOW R. B., LEY R. D. 1971; Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine. Proceedings of the National Academy of Sciences of the United States of America 68:708
    [Google Scholar]
  19. ROSS L. J. N. 1969; Herpes simplex virus: host cell interactions. Ph.D. thesis. University of Birmingham
    [Google Scholar]
  20. ROSS L. J. N., WILDY P., CAMERON K. R. 1971; Formation of small plaques by herpes viruses irradiated with ultraviolet light. Virology 45:808
    [Google Scholar]
  21. RUSSELL W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028
    [Google Scholar]
  22. SAUERBIER W. 1961; The influence of 5-bromodeoxyuridine substitution on u.v. sensitivity, host-cell reactivation, and photoreactivation in T1 and P22 H5. Virology 15:465
    [Google Scholar]
  23. SCHNEWEIS K. E. 1969; Die UV-Inaktivierung von Herpes-simplex-Virus aus 5-Bromdesoxyuridin- und 5-Joddesoxyuridin-behandelten Zellkulturen. Archiv fur die gesamte Virusforschung 28:203
    [Google Scholar]
  24. SETLOW R. 1961; The action spectrum for the reversal of the dimerization of thymine induced by ultraviolet light. Biochimica et biophysica acta 49:237
    [Google Scholar]
  25. SETLOW R., BOYCE R. 1963; The action spectra for ultraviolet-light inactivation of systems containing 5-bromouracil-substituted deoxyribonucleic acid. II. Bacteriophage T4. Biochimica et biophysica acta 68:455
    [Google Scholar]
  26. SETLOW R. B., REGAN J. D., GERMAN J., CARRIER W. L. 1969; Evidence that Xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. Proceedings of the National Academy of Sciences of the United States of’America 64:1035
    [Google Scholar]
  27. SZYBALSKI W. 1961; Properties and applications of halogenated deoxyribonucleic acids. Symposium on Fundamental Cancer Research 15:147
    [Google Scholar]
  28. VANTSIS J. T., WILDY P. 1962; Interaction of herpes virus and HeLa cells: comparison of cell killing and infective center formation. Virology 17:225
    [Google Scholar]
  29. WACKER A., REINHARDT D., CRAMER J. 1965; Unterschiedliche Wirkung von Bromuracildesoxyribosid und Joduracildesoxyribosid bei der Bestrahlung von Herpes Simplex-Viren mit UV-Licht. Die Naturwissenschaften 52:502
    [Google Scholar]
  30. WATSON D. H., SHEDDEN W. I. H., ELLIOTT A., TETSUKA T., WILDY P., BOURGAUX-RAMOISY D., GOLD E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399
    [Google Scholar]
  31. ZÁVADOVÁ Z., ZÅVADA J. 1968; Host-cell repair of ultraviolet-irradiated Pseudorabies virus in chick embryo cells. Acta virologica 12:507
    [Google Scholar]
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