1887

Journal of General Virology

Volume 16, Issue 3

Some Properties of the Particles of Three Tobravirus Isolates Free

Abstract

SUMMARY

When centrifuged in caesium chloride solutions, purified preparations of uncloned cultures of the and strains of tobacco rattle virus contained more than one component but single lesion isolates of each strain contained only one component with buoyant densities of 1.306 and 1.324 g./ml. respectively. The buoyant density of the 5 strain of pea early-browning virus was 1.310. As determined by electron microscopy the diameters of the particle and the core were respectively 23 and 5 nm. for and about 21.5 and 4 nm. for both and 5. The mol. wt of the single virus protein, obtained from each freshly purified virus and estimated by polyacrylamide gel electrophoresis, were 28,500 ( and ) and 24,000 (5). Each virus produced nucleoprotein particles of two predominant lengths and these yielded two RNA species whose mol. wt were proportional to those lengths. The mol. wt (× 10) estimated by polyacrylamide gel electrophoresis were 0.7 and 2.5 (), 1.0 and 2.5 () and 1.3 and 2.5 (5).

At pH 8.6 the electrophoretic mobility of the long particle of was 16% greater than that of the short particles. However, the long particles of have few if any antigenic determinants not possessed by short particles.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology, 1972
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1972-09-01
2021-10-23
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References

  1. BARNETT O. W., MURANT A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435
     [Google Scholar]
  2. BISHOP D. H. L., CLAYBROOK J. R., SPIEGELMAN S. 1967; Electrophoretic separation of viral nucleic acids on Polyacrylamide gels. Journal of Molecular Biology 26:373
     [Google Scholar]
  3. BRAKKE M. K. 1960; Density gradient centrifugation and its application to plant viruses. Advances in Virus Research 7:193
     [Google Scholar]
  4. CARPENTER J. M., COOK S. M., GIBBS A. J. 1971; Molecular weights of plant virus proteins. Report Rotham-sted Experimental Station 1970:122
     [Google Scholar]
  5. CRAWFORD L. V. 1960; A study of the Rous sarcoma virus by density gradient centrifugation. Virology 12:143
     [Google Scholar]
  6. FINCH J. T. 1965; Preliminary X-ray diffraction studies on tobacco rattle and barley stripe mosaic viruses. Journal of Molecular Biology 12:612
     [Google Scholar]
  7. GIBBS A. J., HARRISON B. D. 1964; A form of pea early-browning virus found in Britain. Annals of Applied Biology 54:1
     [Google Scholar]
  8. GIBBS A. J., McINTYRE G. A. 1970; A method for assessing the size of a protein from its composition: its use in evaluating data on the size of the protein subunits of plant virus particles. Journal of General Virology 9:51
     [Google Scholar]
  9. GINOZA W., ATKINSON D. E. 1955; Comparison of some physical and chemical properties of eight strains of tobacco mosaic virus. Virology 1:253
     [Google Scholar]
  10. HARRISON B. D., FINCH J. T., GIBBS A. J., HOLLINGS M., SHEPHERD R. J., VALENTA V., WETTER C. 1971; Sixteen groups of plant viruses. Virology 45:356
     [Google Scholar]
  11. HARRISON B.D., NIXON H. L. 1959a; Separation and properties of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569
     [Google Scholar]
  12. HARRISON B. D., NIXON H. L. 1959b; Some properties of infective preparations made by disrupting tobacco rattle virus with phenol. Journal of General Microbiology 21:591
     [Google Scholar]
  13. HARRISON B. D., NIXON H. L., WOODS R. D. 1965; Lengths and structure of particles of barley stripe mosaic virus. Virology 26:284
     [Google Scholar]
  14. HARRISON B. D., ROBERTS I. M. 1968; Association of tobacco rattle virus with mitochondria. Journal of General Virology 3:121
     [Google Scholar]
  15. HARRISON B. D., WOODS R. D. 1966; Serotypes and particle dimensions of tobacco rattle virus from Europe and North America. Virology 28:610
     [Google Scholar]
  16. KITAJIMA E. W., COSTA A. S. 1969; Association of pepper ringspot virus (Brazilian tobacco rattle virus) and host cell mitochondria. Journal of General Virology 4:177
     [Google Scholar]
  17. LESNAW J. A., REICHMANN M. E. 1970; Determination of molecular weights of plant viral protein subunits by Polyacrylamide gel electrophoresis. Virology 42:724
     [Google Scholar]
  18. LOENING U. E. 1967; The fractionation of high molecular weight ribonucleic acid by Polyacrylamide gel electrophoresis. BiochemicalJournal 103:251
     [Google Scholar]
  19. LOENING U. E. 1968; Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355
     [Google Scholar]
  20. LWOFF A., HORNE R. W., TOURNIER P. 1962; A system of viruses. Cold Spring Harbour Symposium 27:51
     [Google Scholar]
  21. MAAT D. Z. 1963; Pea early-browning virus and tobacco rattle virus - two different, but serologically related viruses. Netherlands Journal of Plant Pathology 69:287
     [Google Scholar]
  22. MAYO M. A., MURANT A. F., HARRISON B. D. 1971; New evidence on the structure of nepoviruses. Journal of General Virology 12:175
     [Google Scholar]
  23. MIKI T., KNIGHT C. A. 1968; The protein subunit of potato virus X. Virology 36:168
     [Google Scholar]
  24. MIKI T., OKADA Y. 1970; Comparative studies on some strains of tobacco rattle virus. Virology 42:993
     [Google Scholar]
  25. NARAYAN K. A., VOGEL M., LAWRENCE J. M. 1965; Disc electrophoresis of wheat flour proteins with a modified apparatus utilizing gels of rectangular cross section. Analytical Biochemistry 12:526
     [Google Scholar]
  26. NIXON H. L., HARRISON B. D. 1959; Electron microscopic evidence on the structure of the particles of tobacco rattle virus. Journal of General Microbiology 21:582
     [Google Scholar]
  27. OFFORD R. E. 1966; Electron microscopic observations on the structure of tobacco rattle virus. Journal of Molecular Biology 17:370
     [Google Scholar]
  28. OFFORD R. E., HARRIS J.I. 1965; The protein subunit of tobacco rattle virus. Federation of European Biochemical Societies. Abstracts 2nd Meeting, Vienna216
     [Google Scholar]
  29. POLSON A., CRAMER R. 1958; Zone electrophoresis of type I poliomyelitis virus. Biochimica et biophysica acta 29:187
     [Google Scholar]
  30. REGENMORTEL M. H. V. VAN. 1968; Zone electrophoresis R0 values of plant viruses. South African Medical Journal 42:118
     [Google Scholar]
  31. RINGBORG U., DANEHOLT B., EDSTROM J. E., EGYHAZI E., LAMBERT B. 1970; Electrophoreticcharacterization of nucleolar RNA from Chironomus tentans salivary gland cells. Journal of Molecular Biology 51:327
     [Google Scholar]
  32. SEMANCIK J. S. 1966; Purification and properties of two isolates of tobacco rattle virus from pepper in California. Phytopathology 56:1190
     [Google Scholar]
  33. SEMANCIK J. S. 1970; Identity of structural protein from two isolates of TRV with different length of associated short particles. Virology 40:618
     [Google Scholar]
  34. SIEGEL A., HUDSON W. 1959; Equilibrium centrifugation of two strains of tobacco mosaic virus in density gradients. Biochimica et biophysica acta 34:254
     [Google Scholar]
  35. SZYBALSKI W. 1968 Equilibrium sedimentation of viruses, nucleic acids and other macro molecules in density gradients. Fractions 11 Published by Spinco division of Beckman Instruments Inc.; Palo Alto, California:
     [Google Scholar]
  36. TOLLIN P., WILSON H. R. 1971; Some observations on the structure of the Campinas strain of tobacco rattle virus. Journal of General Virology 13:433
     [Google Scholar]
  37. WEBER K., OSBORN M. 1969; The reliability of molecular weight determinations by dodecyl sulphate -Polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406
     [Google Scholar]
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Some Properties of the Particles of Three Tobravirus Isolates
J. Gen. Virol. 16, 285 (1972); https://doi.org/10.1099/0022-1317-16-3-285
/content/journal/jgv/10.1099/0022-1317-16-3-285
/content/journal/jgv/10.1099/0022-1317-16-3-285
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