1887

Abstract

SUMMARY

The behaviour and properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus are described. The mutant multiplied well at 21° but, unlike the wild-type, only slightly at 32° although considerable amounts of uncoated RNA accumulated in inoculated leaves at this temperature. The specific infectivity of the mutant was much lower than that of the wild-type virus because the largest species of encapsidated mutant RNA was almost completely degraded even in virus from plants grown at 21°. The temperature sensitivity and low specific infectivity of the mutant were related to properties of its coat protein, which was much less heat stable than that of the wild-type virus. Glutamic acid and alanine replaced lysine and valine respectively in the mutant coat protein and these replacements, in addition to affecting thermal stability, influenced the polymerization of isolated protein at pH 6.7.

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/content/journal/jgv/10.1099/0022-1317-16-1-69
1972-07-01
2024-10-12
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References

  1. BANCROFT J.B. 1971; The significance of the multicomponent nature of cowpea chlorotic mottle virus RNA. Virology 45:830
    [Google Scholar]
  2. BANCROFT J. B., FLACK I. H. 1972; The behaviour of cowpea chlorotic mottle virus in CsCl. Journal of General Virology 15:247
    [Google Scholar]
  3. BANCROFT J. B., HIEBERT E. 1967; Formation of an infectious nucleoprotein from protein and nucleic acid isolated from a small spherical virus. Virology 32:354
    [Google Scholar]
  4. BANCROFT J. B., HIEBERT E., REES M. W., MARKHAM R. 1968; Properties of cowpea chlorotic mottle virus, its protein and nucleic acid. Virology 34:224
    [Google Scholar]
  5. BANCROFT J. B., HILLS G. J., MARKHAM R. 1967; A study of the self-assembly process in a small spherical virus. Formation of organised structures from protein subunits in vitro. Virology 31:354
    [Google Scholar]
  6. BANCROFT J. B., MCLEAN G. D., REES M. W., SHORT MARGARET N. 1971; The effect of an arginyl to a cysteinyl replacement on the uncoating behaviour of a spherical plant virus. Virology 45:707
    [Google Scholar]
  7. BANCROFT J. B., WAGNER G. W., BRACKER C. E. 1968; The self-assembly of a nucleic-acid free pseudo-top component for a small spherical virus. Virology 36:146
    [Google Scholar]
  8. HARIHARASUBRAMANIAN V., ZAITLIN M., SIEGEL A. 1970; A temperature-sensitive mutant of TMV with unstable coat protein. Virology 40:579
    [Google Scholar]
  9. HEBERT T. T. 1963; Precipitation of plant viruses by polyethylene glycol. Phytopathology 53:362
    [Google Scholar]
  10. JOCKUSCH H. 1964; In vivo-und-in vitro-Verhalten temperatursensitive Mutanten des Tabakmosaikvirus. Zeitschrift fur Verebungslehre 95:379
    [Google Scholar]
  11. JOCKUSCH H. 1966a; Temperatursensitive Mutanten des Tabakmosaikvirus. I. In vivo Verhalten. Zeitschrift fur Verebungslehre 98:320
    [Google Scholar]
  12. JOCKUSCH H. 1966b; Temperatursensitive Mutanten des Tabakmosaikvirus. IL In vitro-Verhalten. Zeitschrift fur Verebungslehre 98:344
    [Google Scholar]
  13. JOCKUSCH H. 1966c; Relations between temperature sensitivity amino acid replacements, and quarternary structure of mutant proteins. Biochemical and Biophysical Research Communications 24:577
    [Google Scholar]
  14. JOCKUSCH H. 1968; Two mutants of tobacco mosaic virus temperature-sensitive in two different functions. Virology 35:94
    [Google Scholar]
  15. JOCKUSCH H., KOBERSTEIN R., JAENICKE R. 1969; Thermal denaturation equilibria of TMV coat proteins with chemically defined differences in their primary structure. Zeitschrift fur Naturforschung 24b:613
    [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  17. LOENING U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  18. REINDEL F., HOPPE W. 1954; A colour method for staining amino acids, peptides and proteins on paper-chromatograms and paper electrophoretograms. Chemische Berichte 87:1103
    [Google Scholar]
  19. SIEGEL A. 1960; Studies on the induction of tobacco mosaic virus mutants with nitrous acid. Virology 11:156
    [Google Scholar]
  20. SMYTH D. G. 1967 Techniques in enzymic hydrolyses. Methods in Enzymology 11114 Ed. by Hirs C. H. W. New York: Academic Press;
    [Google Scholar]
  21. SPIES J. R., CHAMBERS D. C. 1949; Chemical determination of tryptophan in proteins. Analytical Chemistry 21:1249
    [Google Scholar]
  22. WITTMANN H. G., WITTMANN-LIEBOLD B. 1966; Protein chemical studies of two RNA viruses and their mutants. Cold Spring Harbor Symposia on Quantitative Biology 31:163
    [Google Scholar]
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