1887

Abstract

SUMMARY

A microelectrophoresis technique has been used to study purified suspensions of buffalopox, vaccinia, variola and alastrim viruses. The electrophoretic behaviour of purified, but otherwise untreated buffalopox and vaccinia viruses was indistinguishable. Treatment with trypsin, lipase or -toluene sulphonyl chloride altered the electrophoretic behaviour indicating that the virus surface is lipoprotein, but again, the viruses were indistinguishable. After treatment with 2-mercaptoethanol differences were found in the electrophoretic behaviour of buffalopox and vaccinia viruses. Preliminary experiments showed that cores, extracted from the viruses, also differed in electrophoretic behaviour.

The electrophoretic behaviour of variola virus was indistinguishable from that of alastrim virus even after treatment with 2-mercaptoethanol. However, further experiments indicated that the cores of these two viruses also differed in surface chemical composition.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-16-1-39
1972-07-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/16/1/JV0160010039.html?itemId=/content/journal/jgv/10.1099/0022-1317-16-1-39&mimeType=html&fmt=ahah

References

  1. BAXBY D., HILL B. J. 1971; Characteristics of a new poxvirus isolated from Indian buffaloes. Archiv fur die Gesamte Virusforschung 35:70
    [Google Scholar]
  2. BEDSON H. S., DUMBELL K. R. 1961; The effect of temperature on the growth of pox viruses in the chick embryo. Journal of Hygiene, Cambridge 59:457
    [Google Scholar]
  3. COHEN G. H., WILCOX W. C. 1968; Soluble antigens of vaccinia-infected mammalian cells. III. Relation of ‘early’ and ‘late’ proteins to virus structure. Journal of Virology 2:449
    [Google Scholar]
  4. COHEN S. S. 1945; The chemical alteration of a bacterial surface with special reference to the agglutination of B. proteus OX-19. Journal of Experimental Medicine 82:133
    [Google Scholar]
  5. DOUGLAS H. W. 1957; Electrophoretic studies on spores and vegetative cells of certain strains of B. mega-terium, B. subtilis and. B. cereus. Journal of Applied Bacteriology 20:390
    [Google Scholar]
  6. DOUGLAS H. W. 1959; Electrophoretic studies on bacteria. Part 5. Interpretation of the effects of pH and ionic strength on the surface charge borne by B. subtilis spores with some observations on other organisms. Transactions of the Faraday Society 55:850
    [Google Scholar]
  7. DOUGLAS H. W., RONDLE C J. M., WILLIAMS B. L. 1966; Microelectrophoresis of cowpox and vaccinia viruses in molar sucrose. Journal of General Microbiology 42:107
    [Google Scholar]
  8. DOUGLAS H. W., WILLIAMS B. L., RONDLE C. J. M. 1969; Micro-electrophoresis of poxviruses in molar sucrose. Journal of General Virology 5:391
    [Google Scholar]
  9. EASTERBROOK K. B. 1966; Controlled degradation of vaccinia virus in vitro. Journal of Ultrastructural Research 14:484
    [Google Scholar]
  10. FENNER F., SAMBROOK J. F. 1966; Conditional lethal mutants of rabbitpox virus. II. Mutants (P) which fail to multiply in PK-2a cells. Virology 28:600
    [Google Scholar]
  11. HARRIS W. J., WESTWOOD J. C N. 1964; Phosphotungstate staining of vaccinia virus. Journal of General Microbiology 34:491
    [Google Scholar]
  12. HILL B. J. 1971; Biological, serological and electrophoretic characteristics of poxviruses isolated from Indian buffaloes. Ph.D. Thesis, Liverpool University
    [Google Scholar]
  13. JOKLIK W. K. 1966; The poxviruses. Bacteriological Reviews 30:33
    [Google Scholar]
  14. RATES J. R., MCAUSLAN B. R. 1967; Poxvirus DNA-dependent RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 58:134
    [Google Scholar]
  15. MITCHENER M. B. 1969; The envelope of vaccinia and orf viruses: an electron-cytochemical investigation. Journal of General Virology 5:211
    [Google Scholar]
  16. WESTWOOD J. C. N., HARRIS W. I., ZWARTOUW H. T., TITMUSS D. H., APPLEYARD O. 1964; Studies on the structure of vaccinia virus. Journal of General Microbiology 34:67
    [Google Scholar]
  17. WOODSON B. 1968; Recent progress in poxvirus research. Bacteriological Reviews 32:127
    [Google Scholar]
  18. ZWARTOUW H. T., WESTWOOD J. C N., HARRIS W. J. 1965; Antigens from vaccinia virus particles. Journal of General Microbiology 38:39
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-16-1-39
Loading
/content/journal/jgv/10.1099/0022-1317-16-1-39
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error