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Abstract
The proteins from bacteriophage Ø29 have been dissociated with 1% SDS and 2% 2-mercaptoethanol and have been examined by SDS-acrylamide gel electrophoresis. Seven different polypeptides bands have been detected in the purified virus. One of the bands (b 1) is frequently detected in different positions suggesting that it represents an aggregate rather than a very large polypeptide. The molecular weights of the other polypeptide bands (b2, b3, b3–4, b4, b5 and b6) estimated by co-electrophoresis with marker proteins are 93,000; 75,000; 65,000; 53,000; 41,000 and 30,000. Protein bands b2, b4 and b6 compose 80% of the bacteriophage. The six structural polypeptides described here could account for approximately 60% of the information contained in the virus DNA.
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