The proteins from bacteriophage φ29 have been dissociated with 1% SDS and 2% 2-mercaptoethanol and have been examined by SDS-acrylamide gel electrophoresis. Seven different polypeptides bands have been detected in the purified virus. One of the bands ( ) is frequently detected in different positions suggesting that it represents an aggregate rather than a very large polypeptide. The molecular weights of the other polypeptide bands (b, b, b, b, b and b) estimated by co-electrophoresis with marker proteins are 93,000; 75,000; 65,000; 53,000; 41,000 and 30,000. Protein bands b, b and b compose 80% of the bacteriophage. The six structural polypeptides described here could account for approximately 60% of the information contained in the virus DNA.


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