1887

Abstract

SUMMARY

BHK cells transformed by polyoma virus have been shown to release protein into the medium which is more glycosylated than that released by normal BHK cells. Correspondingly the residual protein in all membrane fractions (particularly plasma and smooth reticulum membranes) has a lower degree of glycosylation in transformed cells. The glycoproteins in these fractions and in saline washes of normal and transformed cells have been compared by electrophoresis in acrylamide gels. The washes from transformed cells contain a component of molecular weight about 135,000 which is more highly glycosylated than the corresponding component of normal cells. The corresponding membrane fractions of transformed cells are much less glycosylated. It is suggested that transformation by polyoma virus is accompanied by defects in the synthesis or assembly into the membranes of a large component with a high aminosugar to amino acid content.

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/content/journal/jgv/10.1099/0022-1317-14-2-133
1972-02-01
2024-10-09
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References

  1. Bosmann H. B., Hagopian H., Eylar E. H. 1968; Membrane glycoprotein biosynthesis: change in levels of glycosyl-transferases in fibroblasts transformed by oncogenic viruses. Journal of Cellular Physiology 72:81
    [Google Scholar]
  2. Buck C. A., Glick M. c., Warren L. 1970; A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry 9:4567
    [Google Scholar]
  3. Buck C. A., Glick M. C., Warren L. 1971; Glycopeptides from the surface of control and virus-transformed cells. Science, New York 172:169
    [Google Scholar]
  4. Burger M. M. 1969; A difference in the architecture of the surface of normal and virally transformed cells. Proceedings of the National Academy of Sciences of the United States of America 62:994
    [Google Scholar]
  5. Chauveau L., Moule Y., Rouiller C. H. 1956; Isolation of pure and unaltered liver nuclei: morphology and biochemical composition. Experimental Cell Research 11:317
    [Google Scholar]
  6. Dallner G. A., Bergstrand A., Nillson R. 1968; Heterogeneity of rough-surfaced live microsomal membranes of adult, phenobarbital-treated, and newborn rats. Journal of Cell Biology 38:257
    [Google Scholar]
  7. Dunker A. K., Ruekert R. R. 1969; Observations on molecular weight determination on polyacrylamide gel. The Journal of Biological Chemistry 244:5074
    [Google Scholar]
  8. Hakomori S., Murakami W. T. 1968; Glycolipids of hamster fibroblasts and derived malignant-trans-formed cell lines. Proceedings of the National Academy of Sciences of the United States of America 59:254
    [Google Scholar]
  9. Inbar M., Sachs L. 1969; Structural differences in sites on surface membrane of normal and transformed cells. Nature, London 223:710
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  11. Maizel J. V. Jun 1966; Acrylamide-gel electrophorograms by mechanical fractionation of radioactive adenovirus proteins. Science, New York 151:988
    [Google Scholar]
  12. Meezan E., WU H. C., Black P. H., Robbins P. W. 1969; Comparative studies on carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by Sephadex chromatography. Biochemistry 8:2518
    [Google Scholar]
  13. Mora P. T., Brady R. O., Bradley R. M., McFarland V. W. 1969; Gangliosides in DNA-virus transformed and spontaneously transformed tumorigenic mouse cell lines. Proceedings of the National Academy of Sciences of the United States of America 63:1290
    [Google Scholar]
  14. Reisfeld R. A., Lewis U. J., Williams D. E. 1962; Disk-electrophoresis of basic proteins and peptides on polyacrylamide gel. Nature, London 195:281
    [Google Scholar]
  15. Sheinin R., Onodera K. 1970; Studies on biochemical properties of surface components of normal and SV-40 transformed 3T3 mouse cells. Canadian Journal of Biochemistry 48:851
    [Google Scholar]
  16. Sheinin R., Onodera K., Yogeeswaran G., Murray R. K. 1971; Studies of components of the surface of normal and virus transformed mouse cells. In The Molecular Biology of Animal Tumour Viruses Tooze J. Ed Cold Spring Harbor; New York: 361971
    [Google Scholar]
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