The Bunyamwera supergroup is the largest serogroup of arboviruses. Its members are distributed all over the world and some are medically important. Little is known of the structure and biochemical properties of these viruses. By electron microscopy, partly disintegrated virus particles of two members of this supergroup (Batai and Inkoo) have been reported to liberate coiled structures interpreted as being helical nucleoprotein (von Bonsdorff, Saikku & Oker-Blom, 1969). In this study the nucleoprotein has been isolated from purified virus particles of the Inkoo virus (cryptogram, ), a Finnish subtype of the California encephalitis virus (J. Casals, personal communication).

The 4th to 7th tissue culture passages of the Inkoo virus were grown in BHK 21/WI-2 cells (Vaheri 1965). Cell monolayers in Roux bottles were infected with a multiplicity of 0.3 to 6 p.f.u./cell. The virus was grown in Eagle's minimum essential medium containing 0.2% bovine albumin in the presence of 1 µc/ml. of [H]-uridine or [H]-leucine added at the time of infection (Radiochemical Centre, Amersham, Buckinghamshire, specific activities 25 c/m-mole and 1 c/m-mole, respectively).


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