1887

Abstract

SUMMARY

Two distinct immunogenic sites were present on the surface of foot-and-mouth disease virus. One site was concerned with adsorption of the virus to susceptible cells as well as the production of neutralizing antibody. Removal of this site with trypsin did not alter the gross morphology but the particles then had reduced infectivity, although the virus RNA was present in a fully infective form. The presence of a second immunogenic site was shown by the fact that trypsin-treated particles also produced a neutralizing antibody. This differed from the neutralizing antibody against the intact virus in that it was absorbed by trypsin-treated particles. Some of the neutralizing activity of antiserum produced by inoculation of intact virus particles was also absorbed by trypsin-treated particles. The relative activities of the two antibody-producing sites varied with different strains of virus.

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/content/journal/jgv/10.1099/0022-1317-13-1-85
1971-10-01
2022-01-16
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References

  1. Almeida J. D., Brown F., Waterson A. P. 1967; The morphological characteristics of 19s antibody. Journal of Immunology 98:186
    [Google Scholar]
  2. Basch R. S. 1968; An improved method for counting tritium and carbon-14 in acrylamide gels. Analytical Biochemistry 26:184
    [Google Scholar]
  3. Birnboim H. C. 1970; Optimal conditions for counting of precipitated 3H-RNA on glass-fiber filters. Analytical Biochemistry 37:178
    [Google Scholar]
  4. Brown F. 1960; A β-globulin antibody in the sera of guinea pigs and cattle infected with foot-and-mouth disease. Journal of Immunology 85:298
    [Google Scholar]
  5. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature, London 199:1168
    [Google Scholar]
  6. Brown F., Cartwright B., Stewart D. L. 1963; The effect of various inactivating agents on the viral and the ribonucleic acid infectivities of foot-and-mouth disease virus and on its attachment to susceptible cells. Journal of General Microbiology 31:179
    [Google Scholar]
  7. Brown F., Graves J. H. 1959; Changes in specificity and electrophoretic mobility of the precipitating antibodies present in the serum of cattle recovering from foot-and-mouth disease. Nature, London 183:1688
    [Google Scholar]
  8. Brown F., Smale C. J. 1970; Demonstration of three specific sites on the surface of foot-and-mouth disease virus. Journal of General Virology 7:115
    [Google Scholar]
  9. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:73
    [Google Scholar]
  10. Dulbecco R., Vogt M. 1954; One step growth curve of Western equine encephalomyelitis virus on chick embryo cells grown in vitro and analysis of virus yields from single cells. Journal of Experimental Medicine 99:183
    [Google Scholar]
  11. Graves J. H., Cowan K. M., Trautman R. 1964; Characterization of antibodies produced by guinea pigs inoculated with inactivated foot-and-mouth disease antigen. Journal of Immunology 92:501
    [Google Scholar]
  12. OuchterIony O. 1949; Antigen-antibody reactions in gels. Arkiv for Kemi, Mineralogi och Geologi 26B:1
    [Google Scholar]
  13. Pringle C. R. 1969; Electrophoretic properties of foot-and-mouth disease virus strains and the selection of intra-strain mutants. Journal of General Virology 4:541
    [Google Scholar]
  14. Thorne H. V. 1963; Electrophoretic study of the interaction of radioactive poliovirus with components of cultured cells. Journal of Bacteriology 85:217
    [Google Scholar]
  15. Wild T. F., Brown F. 1967; Nature of the inactivating action of trypsin on foot-and-mouth disease virus. Journal of General Virology 1:247
    [Google Scholar]
  16. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313
    [Google Scholar]
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