1887

Abstract

SUMMARY

The development and constitution of the inclusion bodies induced by Italian and Californian isolates of cauliflower mosaic virus was studied by light and electron microscopy. Cytochemical and enzyme-digestion tests revealed that the inclusion bodies are essentially proteinaceous, and contain RNA and some DNA. These inclusions have a matrix composed of densely packed, finely granular or fibrillar material and vacuole-like areas not bounded by a membrane. Virus particles are interspersed at random with the matrix and are not aggregated in regular crystalline arrays. The developmental sequence of the inclusions suggests that they are not merely structures for virus accumulation but, rather, that they are sites of virus synthesis and/or assembly.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-13-1-133
1971-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/13/1/JV0130010133.html?itemId=/content/journal/jgv/10.1099/0022-1317-13-1-133&mimeType=html&fmt=ahah

References

  1. Bos L. 1969; Inclusion bodies of bean yellow mosaic virus, some less known closely related viruses and beet mosaic virus. Netherlands Journal of Plant Pathology 75:137
    [Google Scholar]
  2. Brunt A. A. 1966; Partial purification, morphology and serology of dahlia mosaic virus. Virology 28:778
    [Google Scholar]
  3. Pujisawa I., Rubio-Huertos M., Matsui C., Yamaguchi A. 1967; Intracellular appearance of cauliflower mosaic virus particles. Phytopathology 57:1130
    [Google Scholar]
  4. Hollings M., Stone O. M. 1969; Carnation viruses. Annual Report Glasshouse Crops Research Institute 1968, Littlehampton p 102
    [Google Scholar]
  5. Jordan B. M., Baker J. R. 1955; A simple pyronin methyl-green technique. Quarterly Journal of Microscopical Science, New York 96:177
    [Google Scholar]
  6. Kamei T., Goto T., Matsui C. 1969; Turnip mosaic virus multiplication in leaves infected with cauliflower mosaic virus. Phytopathology 59:1795
    [Google Scholar]
  7. Kamei T., Rubio-Huertos M., Matsui C. 1969; Thymidine-3H uptake by X-bodies associated with cauliflower mosaic virus infection. Virology 37:506
    [Google Scholar]
  8. Karnovsky M. J. 1965; A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. Journal of Cell Biology 27:137 A
    [Google Scholar]
  9. Kitajima E. W., Oliveira A. R., Costa A. S. 1965; Occorência de virus esférico causando faixa amarela da couve em São Paulo. Bragantia 24:219
    [Google Scholar]
  10. Kitajima E. W., Lauritis J. A., Swift H. 1969; Fine structure of zinnia leaf tissues infected with dahlia mosaic virus. Virology 39:240
    [Google Scholar]
  11. Mamula D., Milicic D. 1968; Über. die Eigenschaften von zwei jugoslawischen Isolaten des Blumen-kohlmosaikvirus. Phytopathologische Zeitschrift 61:232
    [Google Scholar]
  12. Mazia D., Brewer P. A., Alfert M. 1953; The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biological Bulletin 35: C 17
    [Google Scholar]
  13. Mollenhauer H. H. 1964; Plastic embedding mixtures for use in electron microscopy. Stain Technology 39:111
    [Google Scholar]
  14. Moreno R., Castro S., Rubio-Huertos M. 1969; Le virus de l’ œillet en Espagne. Annales de Phyto-pathologie 1 hors sèrie 307
    [Google Scholar]
  15. Petzold H. 1968; Elektronenmikroskopische Untersuchungen an von Dahlienmosaikvirus infizierten Pflanzen. Phytopathologische Zeitschrift 63:201
    [Google Scholar]
  16. Robb S. M. 1964; Location, structure and cytochemical staining reactions of the inclusion bodies found in Dahlia variabilis infected with dahlia mosaic virus. Virology 23:141
    [Google Scholar]
  17. Rubio-Huertos M. 1950; Estudios sobre inclusiones intracellulares producidas por virus en las plantas. Microbiologia Espahola 3:207
    [Google Scholar]
  18. Rubio-Huertos M., Matsui C., Yamaguchi A., Kamei T. 1968; Electron microscopy of X-body formation in cells of cabbage infected with Brassica virus 3. Phytopathology 58:548
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-13-1-133
Loading
/content/journal/jgv/10.1099/0022-1317-13-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error