1887

Abstract

SUMMARY

The kinetics of the liberation of protein components from purified poliovirus was examined under varying alkaline pH conditions at 40°. The proteins of the liberated components and of the virus capsid were analysed by sucrose gradient centrifugation and polyacrylamide gel electrophoresis.

When the virus particle was treated at pH 10.0, a minor component enriched in the capsid protein VP 4 was liberated from the virus capsid and a remaining capsid structure had the same H antigenicity as intact empty capsids free of virus RNA. A second component consisting mainly of VP 2 was released from the capsid at pH 11.0 and the residual capsid contained VP 1 and VP 3. This altered capsid still possessed H antigenicity and was stable at pH 11.0, but was degraded to smaller components at pH 12.0. This smaller component did not show H antigenicity. The results suggested that the basic matrix of the particle structure is composed of VP 1 and VP 3 and it exhibits H antigenicity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-13-1-101
1971-10-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/13/1/JV0130010101.html?itemId=/content/journal/jgv/10.1099/0022-1317-13-1-101&mimeType=html&fmt=ahah

References

  1. Boeyé A., Van Elsen A. 1967; Alkaline disruption of poliovirus: Kinetics and purification of RNA-free particles. Virology 33:335
    [Google Scholar]
  2. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279
    [Google Scholar]
  3. Britton H. T. S., Robinson R. A. 1931; The use of the antimony-antimonous oxide electrode in the determination of the concentration of hydrogen ions and in potentiometric titrations. The prideaux-ward universal buffer mixture. Journal of the Chemical Society,London458
    [Google Scholar]
  4. Hinuma Y., Katagiri S., Fukuda M., Fukushi K., Watanabe Y. 1965; Kinetic studies on the thermal degradation of purified poliovirus. Biken Journal 8:143
    [Google Scholar]
  5. Hinuma Y., Katagiri S., Aikawa S. 1970; Immune responses of H particles of poliovirus. Virology 40:773
    [Google Scholar]
  6. Katagiri S., Hinuma Y., Ishida N. 1967; Biophysical properties of poliovirus particles irradiated with ultraviolet light. Virology 32:337
    [Google Scholar]
  7. Katagiri S., Hinuma Y., Ishida N. 1968; Relation between the adsorption to cells and antigenic properties in poliovirus particles. Virology 34:797
    [Google Scholar]
  8. Maizel J. V. 1963; Evidence for multiple components in the structural protein of type 1 poliovirus. Biochemical and Biophysical Research Communications 13:483
    [Google Scholar]
  9. Maizel J. V., Phillips B. A., Summers D. F. 1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type 1. Virology 32:692
    [Google Scholar]
  10. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proceedings of the National Academy of Science of the United States of America 45:505
    [Google Scholar]
  11. Van Elsen A., Boeye A. 1966; Disruption of type i poliovirus under alkaline conditions: Role of pH, temperature, and sodium dodecyl sulfate (SDS). Virology 28:481
    [Google Scholar]
  12. Watanabe Y., Watanabe K., Katagiri S., Hinuma Y. 1965; Virus-specific proteins produced in HeLa cells infected with poliovirus: Characterization of subunit-like protein. Journal of Biochemistry 57:733
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-13-1-101
Loading
/content/journal/jgv/10.1099/0022-1317-13-1-101
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error