1887

Abstract

Summary

Purified preparations of adenovirus were readily disrupted by heating them for a short time in the presence of sodium deoxycholate. The inner complex of proteins and virus DNA (cores) was partially purified by velocity gradient centrifugation in sucrose or glycerol. Further purification was achieved by equilibrium centrifugation on a preformed gradient of ‘Urografin’. Acrylamide gel electrophoresis showed that the cores contained principally two protein components one of which could be removed at the last stage of purification. The core preparations were infective at an extremely low efficiency, the infectivity being sensitive to DNase, trypsin and to various antivirus sera.

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1971-04-01
2022-01-20
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