1887

Abstract

Summary

The molecular weights of the protein subunits of ringspot virus, belladonna mottle virus and brome mosaic virus were estimated by the equilibrium centrifugation method of Yphantis to be 17,300, 20,700 and 17,200, respectively.

Solutions of the virus protein subunit monomers were prepared by suspending the virus particles in neutral buffers containing 6-urea and Cleland’s reagent; preliminary tests with well-characterized proteins showed that the urea solution decreased the partial specific volume of the proteins by about 0.02 to 0.03 g./ml.

In solvents without urea brome mosaic virus dimer subunits had a molecular weight of about 40,000.

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1971-04-01
2022-09-25
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References

  1. Galen W.L. 1966; The significance of the ‘partial specific volume’ obtained from sedimentation data. Biochemistry 5:2553
    [Google Scholar]
  2. Gibbs A.J., Mcintyre G.A. 1970; A method for assessing the size of a protein from its composition: its use in evaluating data on the size of the protein subunits of plant virus particles. Journal of General Virology 9:51
    [Google Scholar]
  3. Hiebert E., Bancroft J.B., Bracker C.E. 1968; The assembly in vitro of some small spherical viruses, hybrid viruses, and other nucleoproteins. Virology 34:492
    [Google Scholar]
  4. Jankulowa M., Huyh W., Wittmann H.G., Paul H.L. 1968; Untersuchungen über ein neues isometrisches Virus aus Atropa belladonna L. II. Phytopathologische Zeitschrift 63:177
    [Google Scholar]
  5. Kaper J.W., Litjens E.C. 1966; The ribonucleic acid content of turnip yellow mosaic virus. Biochemistry 5:1612
    [Google Scholar]
  6. Kawahara K., Tanford C. 1966; Viscosity and density of aqueous solutions of urea and guanidine hydrochloride. Journal of Biological Chemistry 241:3228
    [Google Scholar]
  7. Kielley W.W., Harrington W.F. 1960; A model for the myosin molecule. Biochimica et biophysica acta 41:401
    [Google Scholar]
  8. Koenig R., Stegemann H., Francksen H., Paul H.L. 1970; Protein subunits in the potato virus X group. Determination of the molecular weights by polyacrylamide electrophoresis. Biochimica et biophysica acta 207:184
    [Google Scholar]
  9. Marler E., Nelson C.A., Tanford C. 1964; The polypeptide chains of rabbit γ-globulin and its papain-cleaved fragments. Biochemistry 3:279
    [Google Scholar]
  10. Paul H.L., Bode O., Jankulowa M., Brandes J. 1968; Untersuchungen fiber ein neues isometrisches Virus aus Atropa belladonna L. I.. Phytopathologisehe Zeitschrift 6I:342
    [Google Scholar]
  11. Paul H.L., Huth W. 1970; Untersuchungen fiber das cocksfoot mild mosaic virus. II. Phytopathologisehe Zeitschrift 69:1
    [Google Scholar]
  12. Paul H.L., Wetter C., Wittmann H.G., Brandes J. 1965; Untersuchungen am Odontoglossum ringspot virus, einem Verwandten des Tabakmosaik-Virus. I. Zeitschrift füt Vererbungslehre 97:186
    [Google Scholar]
  13. Reisler E., Eisenberg H. 1969; Interpretation of equilibrium sedimentation measurements of proteins in guanidine hydrochloride solutions. Partial volumes, density increments, and the molecular weight of the subunits of rabbit muscle aldolase. Biochemistry 8:4572
    [Google Scholar]
  14. Stubbs J.D., Kaesberg P. 1964; A protein subunit of brome-grass mosaic virus. Journal of Molecular Biology 8:314
    [Google Scholar]
  15. Teller D.C., Horbett T.A., Richards E.G., Schachman H.K. 1969; Ultracentrifuge studies with Rayleigh interference optics. III. Annals of the New York Academy of Sciences 164:66
    [Google Scholar]
  16. Ullmann A., Goldberg M.E., Perrin D., Monod J. 1968; On the determination of molecular weight of proteins and protein subunits in the presence of 6 M-guanidine hydrochloride. Biochemistry 7:261
    [Google Scholar]
  17. Weber F.N.JUN., Elton R.M., Kim H.G., Rose R.D., Steere R.L., Kupke D.W. 1963; Equilibrium sedimentation of uniform rods of tobacco mosaic virus. Science, New York 140:1090
    [Google Scholar]
  18. Yphantis D.A. 1964; Equilibrium ultracentrifugation of dilute solutions. Biochemistry 3:297
    [Google Scholar]
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