1887

Abstract

SUMMARY

Primary cell cultures and a line consisting of fibroblast-type cells were obtained from tadpoles of the common frog, . One cell line was taken through 21 subcultures during 5 months, when it was abandoned due to contamination by an anonymous which was completely resistant to antibiotics. Cells from the primary cultures supported the growth of three tick-borne arboviruses without any cytopathic effect; Quaranfil, louping ill (two strains), and Langat. One of the virus strains, louping ill 3692, was serially passaged in the cell cultures 11 times in 11 weeks, during which time there was considerable multiplication of virus.

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/content/journal/jgv/10.1099/0022-1317-10-2-131
1971-02-01
2024-04-26
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References

  1. Auclair W. 1961; Cultivation of monolayer cultures of frog renal cells. Nature, London 192:467
    [Google Scholar]
  2. Clark H. F., Karzon D. T. 1967; Terrapene heart (TH-i), a continuous cell line from the heart of the box turtle Terrapene Carolina. Experimental Cell Research 48:263
    [Google Scholar]
  3. Falcoff E., Fauconnier B. 1965; In vitro production of an interferon-like inhibitor of viral multiplication by a poikilothermic animal cell, the tortoise (Testudo graeca). Proceedings of the Society for Experimental Biology and Medicine 118:609
    [Google Scholar]
  4. Freed J. J., Freed L. 1970; Stable haploid cultured cell lines from frog embryos. Proceedings of the National Academy of Sciences of the United States of America 65:337
    [Google Scholar]
  5. Kroeker H. M., Bird T. J. 1965; Use of Carassius auratus cell lines for virus studies. Bacteriological Proceedings 137:120
    [Google Scholar]
  6. Officer J. E. 1964; Ability of a fish cell line to support the growth of mammalian viruses. Proceedings of the Society for Experimental Biology and Medicine 116:190
    [Google Scholar]
  7. Rafferty K. A. Jun 1965; The cultivation of inclusion-associated viruses from Lucke tumour frogs. Annals of the New York Academy of Sciences 126:3
    [Google Scholar]
  8. Shindarov L. 1962a; Propagation of parainfluenza i virus in tortoise kidney cell cultures. Acta virologica 6:540
    [Google Scholar]
  9. Shindarov L. 1962b; Vermehrung des Vakzinevirus in Gewebekultur vom Kaltblüter. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung I. Originale 187:285
    [Google Scholar]
  10. Shindarov L., Sawow Z. 1964; Züchtung des Virus der Vesikulären Stomatitis auf Gewebekultur von Kaltblütern ( Testudo graeca). Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung I. Originale 192:420
    [Google Scholar]
  11. Shindarov L., Todorov S. V. 1962; Kultivierung von Newcastle-Virus in Nierengewebekultur der Schild-kröte (Testudo graeca). Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung I. Originale 186:495
    [Google Scholar]
  12. Somogyiova J., Rehacek J. 1965; Cultivation of tick-borne encephalitis virus in reptile tissue cultures. Acta virologica 9:286
    [Google Scholar]
  13. Soret M. O., Sanders M. 1954; In vitro method for cultivating Eastern equine encephalomyelitis virus in teleost embryos. Proceedings of the Society for Experimental Biology and Medicine 87:526
    [Google Scholar]
  14. Thompson W. R. 1947; Use of moving averages and interpolation to estimate median-effective dose. Bacteriological Reviews 11:115
    [Google Scholar]
  15. Varma M. G. R., Pudney M. 1967; The culture of embryonic cells from the bug Triatoma maculata (Erich-son) (Hemiptera: Reduviidae). Experimental Cell Research 45:671
    [Google Scholar]
  16. Wolf K., Quimby M. C. 1964; Amphibian cell culture. Permanent cell line from the bullfrog (liana catesbeiana). Science, New York 144:1578
    [Google Scholar]
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