1887

Abstract

SUMMARY

After heating at 37° in minimal Eagle’s medium, the alternating polyriboadenylic-polyribouridylic acid [poly r(A-U)] became much more active in producing cellular resistance to virus infection and interferon production in four different cell lines (human skin fibroblasts, mouse embryo fibroblasts, mouse L 929 cells and rabbit kidney (RK 13) cells). The thermal activating effect was neutralized by pre-treatment of the cells with DEAE-dextran, which increased the antiviral activity of unheated and decreased the activity of heated poly r(A-U) to the same level. Under all conditions tested, the degree of cellular resistance to virus infection closely paralleled the amounts of interferon produced suggesting that interferon production is responsible for the antiviral resistance produced by poly r(A-U).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-10-2-125
1971-02-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/10/2/JV0100020125.html?itemId=/content/journal/jgv/10.1099/0022-1317-10-2-125&mimeType=html&fmt=ahah

References

  1. Bausek G. H., Merigan T. C. 1969; Cell interaction with a synthetic polynucleotide and interferon production in vitro . Virology 39:491
    [Google Scholar]
  2. Billiau A., Buckler C. E., Dianzani F., Uhlendorf C., Baron S. 1969; Induction of the interferon mechanism by single-stranded RNA: potentiation by polybasic substances. Proceedings of the Society for Experimental Biology and Medicine 132:790
    [Google Scholar]
  3. Burke D. C., Isaacs A. 1960; Interferon: relation to heterologous interference and lack of antigenicity. Acta virologica 4:215
    [Google Scholar]
  4. Colby C., Chamberlin M. J. 1969; The specificity of interferon induction in chick embryo cells by helical RNA. Proceedings of the National Academy of Sciences of the United States of America 63:160
    [Google Scholar]
  5. De Clercq E., Merigan T. C. 1969; Requirement of a stable secondary structure for the antiviral activity of polynucleotides. Nature, London 222:1148
    [Google Scholar]
  6. De Clercq E., Merigan T. C. 1970; Current concepts of interferon and interferon induction. Annual Review of Medicine 21:17
    [Google Scholar]
  7. De Clercq E., Wells R. D., Merigan T. C. 1970a; Increase in antiviral activity of polynucleotides by thermal activation. Nature, London 226:364
    [Google Scholar]
  8. De Clercq E., Wells R. D., Grant R. C., Merigan T. C. 1970b; Thermal inactivation of the antiviral activity of synthetic double-stranded polyribonucleotides. Journal of Molecular Biology (In the Press)
    [Google Scholar]
  9. Dianzani F., Cantagalli P., Gagnoni S., Rita G. 1968; Effect of DEAE-dextran on production of interferon induced by synthetic double-stranded RNA in L cell cultures. Proceedings of the Society for Experimental Biology and Medicine 128:708
    [Google Scholar]
  10. Falcoff E., Perez-Bercoff R. 1969; Interferons humains induits in vitro par des polynucleotides synthétiques. Biochimica et biophysica acta 174:108
    [Google Scholar]
  11. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. 1968; Inducers of interferon and host resistance. V. In vitro studies. Proceedings of the National Academy of Sciences of the United States of America 61:340
    [Google Scholar]
  12. Finkelstein M. S., Bausek G. H., Merigan T. C. 1968; Interferon inducers in vitro: difference in sensitivity to inhibitors of RNA and protein synthesis. Science, New York 161:465
    [Google Scholar]
  13. Friedman R. M. 1964; Role of interferon in viral interference. Nature, London 201:848
    [Google Scholar]
  14. Ho M., Ke Y. H. 1970; The mechanism of stimulation of interferon production by a complexed polyribonucleotide. Virology 40:693
    [Google Scholar]
  15. Lampson G. P., Tytell A. A., Field A. K., Nemes M. M., Hilleman M. R. 1969; Influence of polyamines on induction of interferon and resistance to viruses by synthetic polynucleotides. Proceedings of the Society for Experimental Biology and Medicine 132:212
    [Google Scholar]
  16. Schäfer T. W., Lockart R. Z. Jun 1970; Interferon required for viral resistance induced by (poly rI). (poly rC). Nature, London 226:449
    [Google Scholar]
  17. Tilles I. G. 1970; Diethylaminoethyl-dextran enhancement of interferon induction by a complexed polyribonucleotide. Proceedings of the Society for Experimental Biology and Medicine 133:1334
    [Google Scholar]
  18. Vilček J., No M. H., Friedman-Kien A. E., Krawciw T. 1968; Induction of interferon synthesis by synthetic double-stranded polynucleotides. Journal of Virology 2:648
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-10-2-125
Loading
/content/journal/jgv/10.1099/0022-1317-10-2-125
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error