We recently reported that the two components from polyoma virus DNA which can be separated after preparative ultracentrifugation (1) show different chromatographic properties on hydroxyapatite (2). Analytical ultracentrifugation and electron microscopy established that the fast component (component I, 20 ) consists of twisted circular molecules only, while the slow component consists of a mixture of untwisted circular molecules (component II, 16 ) and linear molecules (component III, 14.5 ) in variable proportion depending on the DNA preparation examined (3). Since components II and III cannot be satisfactorily separated by preparative ultracentrifugation, the nature of the slow component we isolated by this method was still a matter of uncertainty and the basis for its chromatographic separation from the fast component had therefore to be questioned. We report here the results of experiments made to solve this problem.


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