1887

Abstract

SUMMARY

Partially purified preparations of the strain of cocoa mottle leaf virus were inactivated after 10 min. at 6o°, but not after 10 min. at 55°, All undiluted preparations were infective, most were still infective when diluted 1/10 and some at 1/100; they were still infective after storing for 96 hr at 0 to 4°, or 25°,. The virus retained infectivity after freezing but lost some infectivity when frozen in leaves. The virus was precipitated from clarified extracts by adding 150 to 200 g./l. ammonium sulphate. Infective preparations contained straight rod−shaped particles with rounded ends having a mean length of 1430 Å and apparent width 256 to 260 Å.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-4-465
1967-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/4/JV0010040465.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-4-465&mimeType=html&fmt=ahah

References

  1. Attafuah A., Tinsley T. W. 1958; Virus diseases of Adansonia digitata L. (Bombacaceae) and their relation to cocoa in Ghana. Ann. appl. Biol 46:20
    [Google Scholar]
  2. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  3. Brunt A. A., Kenten R. H. 1962; Mechanical transmission of cocoa mottle leaf virus and three distinct isolates of the cocoa swollen shoot virus. Virology 16:199
    [Google Scholar]
  4. Brunt A. A., Kenten R. H. 1963; The use of protein in the extraction of cocoa swollen shoot virus from cocoa leaves. Virology 19:388
    [Google Scholar]
  5. Brunt A. A., Kenten R. H., Nixon H. L. 1964; Some properties of cocoa swollen shoot virus. J. gen. Microbiol 36:303
    [Google Scholar]
  6. Brunt A. A., Kenten R. H., Gibbs A. J., Nixon H. L. 1965; Further studies on cocoa yellow mosaic virus. J. gen. Microbiol 38:81
    [Google Scholar]
  7. Cadman C. H. 1959; Some properties of an inhibitor of virus infection from leaves of raspberry. J. gen. Microbiol 20:113
    [Google Scholar]
  8. Crowdy S. H., Posnette A. F. 1947; Virus diseases of cacao in West Africa. II. Cross-immunity experiments with viruses 1A, 1B and 1C. Ann. appl. Biol 34:403
    [Google Scholar]
  9. Gibbs A. J., Nixon H. L., Woods R. D. 1963; Properties of purified preparations of lucerne mosaic virus. Virology 19:441
    [Google Scholar]
  10. Glendinning D. R., Lego J. T., Lovi N. K., Martinson V. A. 1966; A field experiment in Ghana on the tolerance of cocoa seedlings to cocoa swollen shoot and cocoa mottle leaf viruses. Ann. appl. Biol 57:389
    [Google Scholar]
  11. Hollings M., Stone O. M., Last F. T. 1965; Detection and identification of viruses in mushroom sporophores and mycelium disrupted with ultrasound. Rep. Glasshouse Crops Res. Inst1964 p. 151
    [Google Scholar]
  12. Kenten R. H., Legg J. T. 1965; Observations on the purification and properties of cocoa swollen shoot virus. Ghana J. Sci 5:221
    [Google Scholar]
  13. Legg J. T., Bonney J. K. 1967; Virus research: insect transmission studies. Ann. Rep. Cocoa Res. Inst. Ghana1965-66 in the Press
    [Google Scholar]
  14. Posnette A. F. 1947; Virus diseases of cacao in West Africa. I. Cacao virus 1 A, 1B, 1C, and 1D. Ann. appl. Biol 34:388
    [Google Scholar]
  15. Posnette A. F. 1950; Virus diseases of Cacao in West Africa. VII. Virus transmission by different vector species. Ann. appl. Biol 37:378
    [Google Scholar]
  16. Thresh J. M., Tinsley T. W. 1959; The viruses of cocoa. Tech. Bull. W. Afr. Cocoa Res. Inst7
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-4-465
Loading
/content/journal/jgv/10.1099/0022-1317-1-4-465
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error