1887

Abstract

Summary

DNA was extracted from purified preparations of radioactive polyoma virus labelled with either P or [C]-or [H]thymidine. Two components only (~ 20, and ~ 16 ) were regularly isolated from all DNA preparations after velocity sedimentation. When linear gradients of increasing phosphate concentrations were applied to hydroxyapatite columns loaded with mixtures of the two components, component I (20 ) was always eluted before component II (16 ). When the two components were partially denatured under critical conditions, they were found to bind equally to the hydroxyapatite crystals; but they behaved very differently after being subjected to extensive denaturation, indicating the dependence of the chromatographic properties of the two components on their configuration.

Chromatography on hydroxyapatite was also shown to allow similarly the separation of virus and host cell DNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-3-323
1967-07-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/3/JV0010030323.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-3-323&mimeType=html&fmt=ahah

References

  1. Bernardi G. 1965; Chromatography of nucleic acids on hydroxyapatite. Nature; Lond: 206779
    [Google Scholar]
  2. Bollum F. J. 1959; Thermal conversion of non-priming deoxyribonucleic acid to primer. J. biol. Chem 234:2733
    [Google Scholar]
  3. Bourgaux P. 1964; The fate of polyoma virus in hamster, mouse and human cells. Virology 23:46
    [Google Scholar]
  4. Bourgaux P., Bourgaux-Ramoisy D., Stoker M. 1965; Further studies on transformation by DNA from polyoma virus. Virology 25:364
    [Google Scholar]
  5. Crawford L. V. 1962; The adsorption of polyoma virus. Virology 18:177
    [Google Scholar]
  6. Crawford L. V. 1963; The physical characteristics of polyoma virus. II. The nucleic acid. Virology 19:279
    [Google Scholar]
  7. Crawford L. V. 1965; A study of human papilloma virus DNA. J. molec. Biol 13:362
    [Google Scholar]
  8. Crawford L. V., Black P. H. 1964; The nucleic acid of simian virus 40. Virology 24:388
    [Google Scholar]
  9. Dulbecco R., Vogt M. 1963; Evidence for a ring structure of polyoma virus DNA. Proc. natrt. Acad. Sci., U.S.A 50:236
    [Google Scholar]
  10. Dulbecco R., Hartwell L. H., Vogt M. 1965; Induction of cellular DNA synthesis by polyoma virus. Proc. natn. Acad. Sci. U.S.A 53:403
    [Google Scholar]
  11. Freifelder D., Davison P. F. 1963; Physicochemical studies on the reaction between formaldehyde and DNA. Biophys. J 3:49
    [Google Scholar]
  12. Nomoto M., Narahashi Y., Murakami M. 1960; Proteolytic enzyme of Streptomyces griseus. V. Protective effect of calcium ion on the stability of protease. J. Biochem., Tokyo 48:453
    [Google Scholar]
  13. Sheinin R. 1966; Deoxyribonucleic acid synthesis in cells replicating polyoma virus. Virology 28:621
    [Google Scholar]
  14. Tiselius A., Hjerten S., Levin O. 1956; Protein chromatography on calcium phosphate columns. Archs Biochem. Biophys 65:132
    [Google Scholar]
  15. Vinograd J., Bruner R., Kent R., Weigle J. 1963; Band-centrifugation of macromolecules and viruses in self-generating density gradients. Proc. natn. Acad. Sci. U.S.A 49:902
    [Google Scholar]
  16. Vinograd J., Lebowitz J. 1966; Physical and topological properties of circular DNA. J. gen. Physiol 49:103
    [Google Scholar]
  17. Vinograd J., Lebowitz J., Radloff R., Watson R., Laipis P. 1965; The twisted circular form of polyoma viral DNA. Proc. natn. Acad. Sci. U.S.A 53:1104
    [Google Scholar]
  18. Weil R. 1961; A quantitative assay for a subviral infective agent related to polyoma virus. Virology 14:46
    [Google Scholar]
  19. Weil R. 1963; The denaturation and the renaturation of the DNA of polyoma virus. Proc. natn. Acad. Sci. U.S.A 49:480
    [Google Scholar]
  20. Weil R., Vinograd J. 1963; The cyclic helix and cyclic coil forms of polyoma viral DNA. Proc. natn. Acad. Sci. U.S.A 50:730
    [Google Scholar]
  21. Weil R., Michel M. R., Ruschmann G. K. 1965; Induction of cellular DNA synthesis by polyoma virus. Proc. natn. Acad. Sci. U.S.A 53:1468
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-3-323
Loading
/content/journal/jgv/10.1099/0022-1317-1-3-323
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error