DNA was extracted from purified preparations of radioactive polyoma virus labelled with either P or [C]- or [H]thymidine. Two components only (∼ 20 and ∼ 16 ) were regularly isolated from all DNA preparations after velocity sedimentation. When linear gradients of increasing phosphate concentrations were applied to hydroxyapatite columns loaded with mixtures of the two components, component I (20 ) was always eluted before component II (16 ). When the two components were partially denatured under critical conditions, they were found to bind equally to the hydroxyapatite crystals; but they behaved very differently after being subjected to extensive denaturation, indicating the dependence of the chromatographic properties of the two components on their configuration.

Chromatography on hydroxyapatite was also shown to allow similarly the separation of virus and host cell DNA.


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