Human influenza A2 viruses have been shown to be capable of reactivating ultraviolet-inactivated strains of influenza A viruses of animal origin (1). Reactivation of classical fowl plague virus by strain A2/Singapore/1/57 gives rise to recombinants sharing properties from each of the parents. Subsequent work with this system revealed that when monolayers of chick embryo fibroblasts inoculated with a given amount of ultraviolet-inactivated fowl plague virus are superinfected with varying doses of living influenza A2, the number of reactivated plaques varies directly with the dose of A2 virus. This provides a simple technique for the quantitative assay of the reactivating capacity of influenza A2 and for the study of neutralization of this capacity by antibody. This constitutes the subject of the present report.

Reactivation experiments were conducted essentially as described by Tumova & Pereira (1).


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