1887

Abstract

[

Wounds from various sources such as burns and ulcers which have been in a prolonged state of inflammation and rubor, both exudative and non-exudative, are decidedly prone to becoming infected by various opportunistic and commensal microorganisms, both bacterial and fungal due to the warm moist environment at the wound site (Que ,2019). The typical approach in treating these wounds is to reduce the level of infection and inflammation, thus encouraging an appropriate environment for successful re-epithelisation resulting in faster healing of the wound.

Bacterial cellulose (BC) is an ideal material to produce wound dressings due to the intrinsic properties such as; high chemical stability, high tensile strength and flexibility, large water holding capacity, permeable to gases and liquids, and excellent biocompatibility (Gupta , 2016; Gupta ,2017, Wahid , 2019).

In this study, BC produced by was used as a hydrogel matrix and loaded with a well-known antimicrobial agent- curcumin encapsulated in solubility enhancing carriers, hydroxypropyl- β-cyclodextrin (CUR:HPβCD) (Abbas , 2019, Gupta , 2019). BC was loaded with 2% (w/v) aqueous CUR:HPβCD inclusion complex under constant agitation at 170 rpm at 20°C (room temperature) for 24 hours.

Antimicrobial activity was tested against two representative organisms:

and by disc diffusion method. We have previously reported the antibacterial activity of CUR:HPβCD-loaded BC (Gupta , 2019). The current study is an extension of our ongoing research where we made an attempt of testing the anti-fungal activity of CUR:HPβCD-loaded BC and compared it with anti-bacterial activity. 8mm discs were aseptically cut and placed on TSA plates seeded with overnight cultures of one of the microorganisms under investigation and incubated at 37°C for 24 hours.

The results indicated that and were both susceptible to CUR:HPβCD inclusion complex with an average zone of inhibition of 12.25±2.2mm and 11±1.2mm respectively (n=4, ±=SD, p <0.05). The results against are in accordance with our previously reported findings (Gupta , 2019). Current findings support the wound management applications of CUR:HPβCd-loaded BC hydrogels for chronic wounds.

References

Abbas, M., Hussain, T., Arshad, M., Ansari, A., Irshad, A., Nisar, J., Hussain, F., Masood, N., Nazir, A. and Iqbal, M. (2019). Wound healing potential of curcumin cross-linked chitosan/polyvinyl alcohol. , 140, pp.871-876.

Gupta, A., Keddie, D., Kannappan, V., Gibson, H., Khalil, I., Kowalczuk, M., Martin, C., Shuai, X. and Radecka, I. (2019). Production and characterisation of bacterial cellulose hydrogels loaded with curcumin encapsulated in cyclodextrins as wound dressings. , 118, pp.437-450.

Gupta, A., Low, W., Britland, S., Radecka, I. and Martin, C. (2017). Physicochemical characterisation of biosynthetic bacterial cellulose as a potential wound dressing material. , 2(2), pp.S37-S38.

Gupta, A., Low, W., Radecka, I., Britland, S., Mohd Amin, M. and Martin, C. (2016). Characterisation and in vitro antimicrobial activity of biosynthetic silver-loaded bacterial cellulose hydrogels. , 33(8), pp.725-734.

Que, A., Nguyen, N., Do, N., Nguyen, N., Tran, N. and Le, T. (2019). Infection of burn wound by Aspergillus fumigatus with gross appearance of fungal colonies. , 24, pp.30-32.

Wahid, F., Hu, X., Chu, L., Jia, S., Xie, Y. and Zhong, C. (2019). Development of bacterial cellulose/chitosan based semi-interpenetrating hydrogels with improved mechanical and antibacterial properties. , 122, pp.380-387.

,

Wounds from various sources such as burns and ulcers which have been in a prolonged state of inflammation and rubor, both exudative and non-exudative, are decidedly prone to becoming infected by various opportunistic and commensal microorganisms, both bacterial and fungal due to the warm moist environment at the wound site (Que ,2019). The typical approach in treating these wounds is to reduce the level of infection and inflammation, thus encouraging an appropriate environment for successful re-epithelisation resulting in faster healing of the wound.

Bacterial cellulose (BC) is an ideal material to produce wound dressings due to the intrinsic properties such as; high chemical stability, high tensile strength and flexibility, large water holding capacity, permeable to gases and liquids, and excellent biocompatibility (Gupta , 2016; Gupta ,2017, Wahid , 2019).

In this study, BC produced by was used as a hydrogel matrix and loaded with a well-known antimicrobial agent- curcumin encapsulated in solubility enhancing carriers, hydroxypropyl- β-cyclodextrin (CUR:HPβCD) (Abbas , 2019, Gupta , 2019). BC was loaded with 2% (w/v) aqueous CUR:HPβCD inclusion complex under constant agitation at 170 rpm at 20°C (room temperature) for 24 hours.

Antimicrobial activity was tested against two representative organisms:

and by disc diffusion method. We have previously reported the antibacterial activity of CUR:HPβCD-loaded BC (Gupta , 2019). The current study is an extension of our ongoing research where we made an attempt of testing the anti-fungal activity of CUR:HPβCD-loaded BC and compared it with anti-bacterial activity. 8mm discs were aseptically cut and placed on TSA plates seeded with overnight cultures of one of the microorganisms under investigation and incubated at 37°C for 24 hours.

The results indicated that and were both susceptible to CUR:HPβCD inclusion complex with an average zone of inhibition of 12.25±2.2mm and 11±1.2mm respectively (n=4, ±=SD, p <0.05). The results against are in accordance with our previously reported findings (Gupta , 2019). Current findings support the wound management applications of CUR:HPβCd-loaded BC hydrogels for chronic wounds.

References

Abbas, M., Hussain, T., Arshad, M., Ansari, A., Irshad, A., Nisar, J., Hussain, F., Masood, N., Nazir, A. and Iqbal, M. (2019). Wound healing potential of curcumin cross-linked chitosan/polyvinyl alcohol. , 140, pp.871-876.

Gupta, A., Keddie, D., Kannappan, V., Gibson, H., Khalil, I., Kowalczuk, M., Martin, C., Shuai, X. and Radecka, I. (2019). Production and characterisation of bacterial cellulose hydrogels loaded with curcumin encapsulated in cyclodextrins as wound dressings. , 118, pp.437-450.

Gupta, A., Low, W., Britland, S., Radecka, I. and Martin, C. (2017). Physicochemical characterisation of biosynthetic bacterial cellulose as a potential wound dressing material. , 2(2), pp.S37-S38.

Gupta, A., Low, W., Radecka, I., Britland, S., Mohd Amin, M. and Martin, C. (2016). Characterisation and in vitro antimicrobial activity of biosynthetic silver-loaded bacterial cellulose hydrogels. , 33(8), pp.725-734.

Que, A., Nguyen, N., Do, N., Nguyen, N., Tran, N. and Le, T. (2019). Infection of burn wound by Aspergillus fumigatus with gross appearance of fungal colonies. , 24, pp.30-32.

Wahid, F., Hu, X., Chu, L., Jia, S., Xie, Y. and Zhong, C. (2019). Development of bacterial cellulose/chitosan based semi-interpenetrating hydrogels with improved mechanical and antibacterial properties. , 122, pp.380-387.

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/content/journal/acmi/10.1099/acmi.amrmeds2019.po0012
2019-12-01
2020-01-24
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