Laboratory-based evolution has become a widely used method to explore fundamental questions about evolution as a process, and is also a powerful tool to study the link between genotype and phenotype. We have evolved six populations of E. coli MG1655 by iterative growth and dilution at pH 4.5 over five months, keeping a frozen fossil record of intermediate populations during this process. Whole genome sequencing of the evolved strains revealed many striking similarities in the evolutionary trajectories in the evolved strains; for example, mutations in arcA are common and may cause loss or alteration of function. We are interested in exploring the impact of different parameters on evolutionary trajectories, but as evolution experiments take a long time, we are currently investigating the potential of traDIS to replicate evolution experiments in a relatively short time frame. Since TraDIS provides a measure of relative contributions to fitness of each gene (by comparison of read counts after growth in two conditions), in principle it should be possible to use TraDIS to identify genes whose loss of function provides a fitness benefit. Here we compare the outcome of these two techniques and present our latest results.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error